Cytotoxic evaluation of two chlorine-releasing irrigating solutions on cultured human periodontal ligament fibroblasts

Bolla, Nagesh; Nalli, Sivavenkata Malati; Sujana,; Kumar, Kunal; Ranganathan,; Raj, Sarath

doi:10.4103/2277-8632.108512

Cited by 4 publications

(2 citation statements)

References 4 publications

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“…Also, after a month, its primary disinfection power and reactivity is reduced to one-third. The other disadvantages of this compound are high corrosivity, skin irritation, and storage problems [1,4,8]. Sodium dichloroisocyanurate (NaDCC) is one of the chlorine compounds that can be used as substitute for NaOCl [9].…”

Section: Introductionmentioning

confidence: 99%

NaDCC) and sodium hypochlorite (NaOCL): Modeling, optimization and comparative analysis

Sharafi¹,

Fazlzadeh²,

Pirsaheb³

et al. 2017

Desalination and Water Treatment

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The aim of this study was to evaluate the ability of sodium dichloroisocyanate (NaDCC) and sodium hypochlorite (NaOCL) for wastewater disinfection in a batch system with considering the contact time (5, 10, 15 min) and disinfectant concentration (0.01, 0.02, 0.04, 0.06, 0.08, 0.1, 0.2, 0.4 mg/L). Distilled water was mixed with wastewater to produce a given concentration of bacteria. Pure plate count (PPC) method was used to determine the initial and final concentration of bacteria. Three regression models including first order (FO), first order plus two way interaction (FO + TWI), and second order (SO) were used to fit the experimental data. The differences among group means were analyzed using Kruskal-Wallis and ANOVA analysis. The removal efficiency of NaDCC and NaOCl were 88.7 and 52.38% at an initial concentration of 0.01 mg/L and contact time of 10 min. The results of Kruskal-Wallis test showed that the mean of disinfection efficiencies were statistically different at different concentration of disinfectant (p-value < 0.05). However, there was no statistically significant difference between removal efficiency at different contact time based on one way ANOVA analysis (p-value > 0.05). These results were the same for both disinfectants. The modeling data showed that the best regression model to describe the disinfection mechanism was SO model with R 2 value of about 0.84 for both NaDCC and NaOCl. According to the present study, it can be concluded that the NaDCC is more suitable for water and wastewater disinfection because of higher efficiency at lower contact time and concentration.

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Section: Introductionmentioning

confidence: 99%

NaDCC) and sodium hypochlorite (NaOCL): Modeling, optimization and comparative analysis

Sharafi¹,

Fazlzadeh²,

Pirsaheb³

et al. 2017

Desalination and Water Treatment

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“…Thus, the use of other irrigant solutions, such as chlorhexidine gluconate has been justified (OKINO et al, 2004;FERRAZ et al, 2007;AKGUN et al, 2013;BOLLA et al, 2013), due to its antimicrobial action, substantivity, less odor and cytotoxicity than NaOCl (DAMETTO et al, 2005). However, chlorhexidine in gel and also in an aqueous solution show no potential to dissolve organic matter (OKINO et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Effect of different combinations of papain-based gels on dissolving pulp tissue

Pithon¹,

Santos²,

Oliveira³

et al. 2017

Biosci. J.

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ABSTRACT:The focus of this study was to evaluate the capacity to dissolve pulp tissue of various combinations of papain-based gels and other antimicrobial agents. 105 bovine pulps were used, of standardized sizes, fragmented into 15mm-sized portions and weighed on an analytical balance, divided into 7 groups (n=15): 1 -0.9% Saline Solution (negative control); 2-8% Papain gel; 3-8% Papain gel + 0.5% Chloramine; 4-0.5% Chloramine gel; 5-8% Papain gel + 2% Chlorhexidine; 6-2% Chlorhexidine gel; and 7-5.25% Sodium Hypochlorite solution (positive control). After initial weighing, the pulp fragments were inserted in test tubes for dissolution for time intervals of 30, 60, 90 and 120 minutes, and then weighed again. The data were analyzed using the Kruskal-Wallis and Mann-Whitney tests (p<0.05). In the time interval of 120 minutes the 0.5% chloramine gel demonstrated 64.9% ability to pulp dissolve, followed by 8% papain gel with 61.3%; papain associated with 0.5% chloramine, 58%; and papain associated with 2% chlorhexidine, 55.4%; which showed statistically significant difference with 5.25% Sodium Hypochlorite (p<0.05). All the gels that contained papain and the 0.5% chloramine gel promoted pulp tissue dissolution, however on a significantly lower scale than 5.25% sodium hypochlorite. The 2% chlorhexidine demonstrated no capacity to dissolve pulp, as did the control.

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Cytotoxicity evaluation of root repair materials in human-cultured periodontal ligament fibroblasts

et al. 2014

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Aim:To evaluate the cytotoxicity of three root repair materials, mineral trioxide aggregate (MTA), Endosequence Root Repair Material and Biodentine in human periodontal ligament fibroblasts.Materials and Methods:Periodontal ligament fibroblasts were cultured from healthy premolar extracted for orthodontic purpose. Cells in the third passage were used in the study. The cultured fibroblast cells were placed in contact with root repair materials: (a) Biodentine, (b) MTA, (c) Endosequence, (d) control. The effects of these three materials on the viability of Periodontal ligament (PDL) fibroblasts were determined by trypan blue dye assay after 24 hours and 48-hour time period. Cell viability was determined using inverted phase contrast microscope.Statistical Analysis:Cell viability was compared for all the experimental groups with Wilcoxons matched pair test.Results:At the 24-hour examination period, all the materials showed increased cell viability. At 48-hour time period, there is slight decrease in cell viability. Mineral trioxide aggregate showed statistically significant increase in the cell viability when compared to other root repair materials.Conclusion:Mineral trioxide aggregate was shown to be less toxic to periodontal ligament fibroblasts than Endosequence Root Repair Material and Biodentine.

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Cytotoxic evaluation of two chlorine-releasing irrigating solutions on cultured human periodontal ligament fibroblasts

Cited by 4 publications

References 4 publications

NaDCC) and sodium hypochlorite (NaOCL): Modeling, optimization and comparative analysis

NaDCC) and sodium hypochlorite (NaOCL): Modeling, optimization and comparative analysis

Effect of different combinations of papain-based gels on dissolving pulp tissue

Cytotoxicity evaluation of root repair materials in human-cultured periodontal ligament fibroblasts

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