Cytotoxic Evaluation of Elastomeric Dental Impression Materials on a Permanent Mouse Cell Line and on a Primary Human Gingival Fibroblast Culture

Abstract

The need for clinically relevant in vitro tests of dental materials is widely recognized. Nearly all dental impression materials are introduced into the mouth just after mixing and allowed to set in contact with the oral tissues. Under these conditions, the materials may be toxic to cells or may sensitize the tissues. The aim of the present study is to evaluate the potential cytotoxicity of new preparations of elastomeric dental impression materials: A) four vinylpolysiloxanes: Elite H-D Putty and Elite H-D Light Body (Zhermack, Badia Polesine, Rovigo, Italy); Express Putty and Express Light Body (3M ESPE AG Seefeld, Germany) and B) two polyethers: Impregum Penta and Permadyne Penta L (3M ESPE AG Seefeld, Germany). The cytotoxicity of these impression materials were examined using two different cell lines: Balb/c 3T3 (permanent cell line) and human gingival fibroblasts (primary cell line) and their effects were studied by indirect and direct tests. The direct tests are performed by placing one sample of the impression materials in the centre of the Petri dishes at the time of the seeding of cells. The cell growth was evaluated at the 12th and 24th hours by cell number. The indirect tests were performed by incubating a square of 1 cm diameter impression material in 5 mL of medium at 37 °C for 24 hours (“eluates”). Subconfluent cultures are incubated with “eluates” for 24 hours. The MTT-formazan production is the method used for measuring the cell viability. The results indicate that: a) polyether materials are cytotoxic under both experimental conditions; b) among vinylpolysiloxanes, only Express Light Body (3M ESPE AG Seefeld, Germany) induces clear inhibition of cellular viability of Balb/c 3T3 evaluated by direct and indirect tests and c) the primary cell line is less sensitive to the toxic effect than the permanent cell line.

New dressing materials derived from transgenic flax products to treat long-standing venous ulcers-a pilot study

“…The cytotoxicity of the flax fibers, oil emulsion, and seedcake extract was determined in a culture of Balb/3T3 mouse fibroblasts of the permanent cell line, widely used for acute toxicity in vitro screening of various chemicals and biomaterials 20–22 . The cells were grown in Dulbecco's modified Eagle's (DMEM) medium enriched with 10% calf serum, penicillin, and streptomycin.…”

“…[20][21][22] The cells were grown in Dulbecco's modified Eagle's (DMEM) medium enriched with 10% calf serum, penicillin, and streptomycin. The test material and cells were incubated together in six-well plates at 37 1C in an air atmosphere containing 5% CO 2 .…”

Evaluation of cytotoxicity levels of poly vinyl ether silicone, polyether, and poly vinyl siloxane impression materials: An in vitro study

“…Indirect cytotoxic testing methods were carried out in this study[ 2 12 ] [ Figure 3 ]. NIH/3T3 cells were incubated with polymerized impression materials in 60-mm Petri dishes in 5 ml of culture medium without serum for 15 min, 30 min, 60 min, and 24 th h at 37°C under sterile conditions [ Figure 3 ].…”

“…The elastomers can tear and can be trapped in the gingival sulcus under implants during impression making and cause adverse and toxic reactions when remained in contact for longer periods of time. [ 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 ] There are many reports on cytotoxicity, tissue reactions, and hypersensitivity of various other dental materials. [ 26 27 28 ] The potential cytotoxicity of these materials can be tested either by introducing the cells into the material (direct test) or to the eluted extracts of the impression materials (indirect tests).…”

Heparan sulfates facilitate harmless amyloidogenic fibril formation interacting with elastin-like peptides

“…Balb/c 3T3 embryonic mouse fibroblasts were grown in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco, Grand Island, NY, U.S.A.), supplemented with 10% foetal calf serum (FCS) (Gibco) 59 .…”