Cytotoxic Effects of Resin Components on Cultured Mammalian Fibroblasts

Hanks, C. T.; Strawn, S.E.; Wataha, John C.; Craig, Robert G.

doi:10.1177/00220345910700111201

Cited by 351 publications

(279 citation statements)

References 22 publications

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“…Viability decrease caused by MTA Fillapex exceeded 75% when pure and ½ diluted. The addition of components in MTA Fillapex, such as resins (8), bismuth oxide (9) and other pigments, may have increased the toxic effect of this sealer compared to its precursor, MTA. In a recent study using osteoblast culture, Scelza et al (15) also observed significant cytotoxic levels for MTA Fillapex extracts, when prepared 1 or 7 days after the sealer manipulation.…”

Section: Discussionmentioning

confidence: 99%

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In Vitro Cytotoxicity of White MTA, MTA Fillapex® and Portland Cement on Human Periodontal Ligament Fibroblasts

et al. 2013

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The aim of this study was to compare the in vitro cytotoxicity of white mineral trioxide aggregate (MTA), MTA Fillapex ® and Portland cement (PC) on human cultured periodontal ligament fibroblasts. Periodontal ligament fibroblast culture was established and the cells were used for cytotoxic tests after the fourth passage. Cell density was set at 1.25 x10 4 cells/well in 96-well plates. Endodontic material extracts were prepared by placing sealer/ cement specimens (5x3mm) in 1mL of culture medium for 72 h. The extracts were then serially two-fold diluted and inserted into the cell-seeded wells for 24, 48 and 72 h. MTT assay was employed for analysis of cell viability. Cell supernatants were tested for nitric oxide using the Griess reagent system. MTA presented cytotoxic effect in undiluted extracts at 24 and 72 h. MTA Fillapex ® presented the highest cytotoxic levels with important cell viability reduction for pure extracts and at ½ and ¼ dilutions. In this study, PC did not induce alterations in fibroblast viability. Nitric oxide was detected in extract-treated cell supernatants and also in the extracts only, suggesting presence of nitrite in the soluble content of the tested materials. In the present study, MTA Fillapex displayed the highest cytotoxic effect on periodontal ligament fibroblasts followed by white MTA and PC.

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Section: Discussionmentioning

confidence: 99%

“…Unlike regular MTA, MTA Fillapex was developed as a paste/paste sealer in a formulation that allows its appropriate insertion into the root canal as a conventional endodontic sealer (8,9).…”

Section: Introductionmentioning

confidence: 99%

In Vitro Cytotoxicity of White MTA, MTA Fillapex® and Portland Cement on Human Periodontal Ligament Fibroblasts

et al. 2013

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“…It has been largely reported that HEMA induces apoptosis in vitro after 24 h (Table 3) (Hanks et al 1991, Krifka et al 2012. Moreover, HEMA induces morphological changes in cultured cells, such as human pulp fibroblasts (HPFs) and human gingival epithelial cells.…”

Section: Hema-induced Apoptosis In Vitromentioning

confidence: 99%

HEMA‐induced cytotoxicity: oxidative stress, genotoxicity and apoptosis

2014

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Gallorini M, Cataldi A, di Giacomo V. HEMA-induced cytotoxicity: oxidative stress, genotoxicity and apoptosis.International Endodontic Journal, 47, 813-818, 2014. Dental resin composites consist of organic polymers with inorganic fillers used as bonding resins and direct filling materials in dentine adhesives and as sealing agents for inlays, crowns and orthodontic brackets. Despite various modifications in the formulation, the chemical composition of composite resins includes inorganic filler particles and additives, which are incorporated into a mixture of an organic resin matrix. Among them, 2-hydroxyethylmethacrylate (HEMA) is one of the most frequently used. Several studies have attempted to clarify the mechanisms underlying HEMA cytotoxicity. Most of them support the hypothesis that this compound, once released in the oral environment, increases reactive oxygen species (ROS) production and oxidative DNA damage through double-strand breaks evidenced by in vitro presence of micronuclei. As a consequence, the glutathione detoxifying intracellular pool forms adducts with HEMA through its cysteine motif and inflammation begins to occur: transcription of early genes of inflammation such as tumour necrosis factor a or inducible cyclooxygenase up to the secretion of prostaglandins 2. These phenomena are counteracted by N-acetylcysteine (NAC), a nonenzymatic antioxidant, but not by vitamin E or other antioxidant. Consequently, NAC prevents HEMA-induced apoptosis acting as a direct ROS scavenger. This minireview collects the most significant papers on HEMA and tries to make an overview of its cytotoxicity on different cell types and experimental models.

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“…This view has been supported also by the fact that various components of dentine-bonding agents and restorative resin materials are directly toxic to cells [12,14,15]. Dentine conditioning agents can also be harmful when the pH value is lower than 5.5 and when they approach or come in contact with pulp [16].…”

Section: Introductionmentioning

confidence: 99%

Composite resin restoration and postoperative sensitivity: clinical follow-up in an undergraduate program

Unemori

Matsuya

Akashi

et al. 2001

Journal of Dentistry

100

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Objectives: To analyze the relationship between the cavity depth and liners with postoperative sensitivity of resin composite restorations. Methods: A clinical follow-up was conducted on 319 resin composite restorations made in the ®nal year of an undergraduate program over a 3-year period. Along with the analyses of cavity type, cavity depth, type of pulpal protection and the materials used, the postoperative sensitivity was also examined on each restoration.Results: Thirty-nine percent of the restorations had no protective layer (Group 1). As the depth of the prepared cavities increased, the restorations received one of the three pulpal protection methods; a calcium hydroxide base (Group 2), glass ionomer cement (Group 3), or protection with a calcium hydroxide base in combination with glass ionomer cement (Group 4). The incidence of postoperative sensitivity showed no signi®cant difference among Groups 1, 2 and 3, but was signi®cantly lower in Group 1 than in Group 4. The restorations made in shallow and medium depth cavities demonstrated signi®cantly less-postoperative sensitivity than those made in deep cavities. The newer generation dentine-bonding agents showed a signi®cantly lower incidence of postoperative sensitivity than the early generation group.Conclusions: Postoperative sensitivity in resin composite restorations was not related to the absence of protective layers but increased with the depth of cavities restored with the resin composite. The type of dentine-bonding agents could also be responsible for postoperative sensitivity. q

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Cytotoxic Effects of Resin Components on Cultured Mammalian Fibroblasts

Cited by 351 publications

References 22 publications

In Vitro Cytotoxicity of White MTA, MTA Fillapex® and Portland Cement on Human Periodontal Ligament Fibroblasts

In Vitro Cytotoxicity of White MTA, MTA Fillapex® and Portland Cement on Human Periodontal Ligament Fibroblasts

HEMA‐induced cytotoxicity: oxidative stress, genotoxicity and apoptosis

Composite resin restoration and postoperative sensitivity: clinical follow-up in an undergraduate program

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