Cytotoxic effects of procyanidins from Castanea mollissima Bl. shell on human hepatoma G2 cells in vitro

Zhang, H; Ke, Jiajia; Shao, Tingyu; Li, J; Duan, Yuqing; He, Yuanqing; Zhang, C; Chen, Guangwei; Sun, Guibo; Sun, Xiaobo

doi:10.1016/j.fct.2013.11.026

Cited by 23 publications

(22 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Accumulating studies have revealed that NP toxicity induces oxidative stress through the generation of ROS and stimulates apoptosis in a variety of cell types, including human embryonic stem cells, TM4 SCs and testicular SCs (Choi et al 2014;Gong et al 2009;Kim et al 2006;Kuo et al 2012;Zhang et al 2014). NP activates endoplasmic reticulum stress response, Fas/FasL, MAPKs, Akt and JNK signaling pathways, resulting in changes in cell survival, cell cycle progression and apoptosis (Choi et al 2014;Duan et al 2016c;Lepretti et al 2015;Liu et al 2015;Yao et al 2005).…”

Section: Introductionmentioning

confidence: 99%

4-Nonylphenol induces autophagy and attenuates mTOR-p70S6K/4EBP1 signaling by modulating AMPK activation in Sertoli cells

Duan

Quan

et al. 2017

Toxicology Letters

View full text Add to dashboard Cite

The estrogenic chemical 4-nonylphenol (NP) is known to impair testicular devolopment and spermatogenesis in rodents. The objective of this study was to explore the effects of NP on autophagy induction and AMPK-mTOR signaling pathway in Sertoli cells (SCs), which are the "nursemaid cells" for meiosis of spermatocytes. In this study we exposed 7-week-old male rats to NP by intra-peritoneal injection at 0, 20, 50 or 100 mg/kg body weight/2 days for 20 consecutive days. Our results showed that exposure to NP dose-dependently induces the formation of autophagosomes in SCs, increases the expression of Beclin-1, the conversion of LC3-I to LC3-II and the mRNA expression of Atg3, Atg5, Atg7 and Atg12 in testis, and these effects are concomitant with the activation of AMPK, and the suppression of TSC2-mTOR-p70S6K/4EBP1 signaling cascade in testis. Furthermore, 10 µM Compound C or AMPKα1 siRNA pre-treatment effectively attenuated autophagy and reversed AMPK-mTOR-p70S6K/4EBP1 signaling in NP-treated SCs. Co-treatment with 1 mM AICAR remarkably strengthened NP-induced autophagy and mTOR inhibition in SCs. Together, these data suggest that NP stimulates Sertoli cell autophagy and inhibits mTOR-p70S6K/4EBP1 activity through AMPK activation, which is the potential mechanism responsible for the regulation of testis function and differentiation following NP exposure.

show abstract

Section: Introductionmentioning

confidence: 99%

4-Nonylphenol induces autophagy and attenuates mTOR-p70S6K/4EBP1 signaling by modulating AMPK activation in Sertoli cells

Duan

Quan

et al. 2017

Toxicology Letters

View full text Add to dashboard Cite

show abstract

“…When cells receive intracellular or extracellular signals leading to apoptosis, prominent mitochondrial alterations, including increased membrane permeability, decreased membrane potential, swelling and disruption, can be observed and eventually result in DNA fragmentation and cell death [16]. Thus, currently mitochondrion has been considered as an ideal target to evaluate the anti-apoptotic efficacy of procyanidins [33,34].…”

Section: Discussionmentioning

confidence: 99%

“…The damaged primary hippocampal neurons induced by ELF-EMF may be involved in diverse mechanisms, including cell cycle arrest, induction of apoptosis and/or necrosis cells. Apoptosis is a form of cell death, a programmed sequence of events leads to the elimination of cells without releasing harmful substances into the surrounding area [16]. It is a physiological mode of cell death, which is characterized by morphological changes and extensive DNA fragmentation.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Neuroprotective effects of lotus seedpod procyanidins on extremely low frequency electromagnetic field-induced neurotoxicity in primary cultured hippocampal neurons

Yin

Duan

et al. 2016

Biomedicine & Pharmacotherapy

View full text Add to dashboard Cite

“…23,24,35 T. indica seed extract caused dose dependent inhibition of phaspholipase A and other enzyme activity of snake venom. 25 Zhanq et al 26 reported the in vitro cytotoxic effect of procynadine on human hepatoma G2 cell. Arjunolic acid of T. arjuna caused maximum inhibition of ACP and ALP enzyme activity in the cerebral ganglion of L. acuminata.…”

Section: In Vitro Enzyme Inhibitionmentioning

confidence: 99%

Inhibition Kinetics of Acetylcholinesterase and Phosphatases by the Active Constituents of Terminalia arjuna and Tamarindus indica in the Cerebral Ganglion of Lymnaea acuminata

Soni¹,

Singh²,

Singh³

2017

View full text Add to dashboard Cite

Introduction: Paper demonstrates effect of the active molluscicidal components arjunolic acid (Terminalia arjuna bark) and procynadine (Tamarindus indica seed) on the activity of acetylcholinesterase (AChE) and phosphatases (ACP/ALP) in the cerebral ganglion of snail Lymnaea acuminata. Materials and Methods: Kinetics of AChE/ACP/ ALP inhibition in the cerebral ganglion of snail Lymnaea acuminata was studied via in vivo (40% and 80% of 96 h LC 50 ) and in vitro treatments (0.3 µg to 7.0 µg) of the column purified fractions, arjunolic acid and procynadine. Results: In vivo exposure of procynadine and arjunolic acid significantly inhibit acetylcholinesterase (AChE), acid phosphatase (ACP) and alkaline phosphatase (ALP) activities in the cerebral ganglion of L. acuminata exposed to 80% of 96h LC 50 . In in vitro treatment maximum inhibition in AChE/ACP/ALP activities in the cerebral ganglion of snail were noted when exposed to 7.0 µg of arjunolic acid and 0.9 µg of procynadine. Column purified fraction of T. arjuna bark and T. indica seed caused non-competitive and uncompetitive inhibition of AChE activity, respectively. Column purified fraction and arjunolic acid of T. arjuna bark caused uncompetitive inhibition of ACP while column purified fraction and procynadine of T. indica seed caused competitive inhibition. Competitivenon-competitive inhibition of ALP activity in the cerebral ganglion of L. acuminata was observed after treatment of column purified active components of both plants. Conclusions: The molluscicidal activity of T. arjuna bark (arjunolic acid) and T. indica seed (procynadine) against snail L. acuminata is due to the inhibition of AChE/ACP/ ALP. Their inhibition kinetics against AChE/ACP/ALP, were different in cerebral ganglion of snail.

show abstract

Cytotoxic effects of procyanidins from Castanea mollissima Bl. shell on human hepatoma G2 cells in vitro

Cited by 23 publications

References 63 publications

4-Nonylphenol induces autophagy and attenuates mTOR-p70S6K/4EBP1 signaling by modulating AMPK activation in Sertoli cells

4-Nonylphenol induces autophagy and attenuates mTOR-p70S6K/4EBP1 signaling by modulating AMPK activation in Sertoli cells

Neuroprotective effects of lotus seedpod procyanidins on extremely low frequency electromagnetic field-induced neurotoxicity in primary cultured hippocampal neurons

Inhibition Kinetics of Acetylcholinesterase and Phosphatases by the Active Constituents of Terminalia arjuna and Tamarindus indica in the Cerebral Ganglion of Lymnaea acuminata

Contact Info

Product

Resources

About