Cytotoxic effect of Rosa canina extract on human colon cancer cells through repression of telomerase expression

Abstract

Rosa canina is a member of the genus Rosa that has long been used for medical objectives. Several studies have reported cytotoxic effects of different Rosa species, but there has been only limited investigation of the cytotoxic effect of R. canina. The purpose of the current study was to examine the potential effect of R. canina extract on cell viability, the cell cycle, apoptosis, and the expression of telomerase in human colon cancer (WiDr) cells. The cytotoxic effect of the extract was determined using MTT assay. The mechanism involved in the cytotoxic effect of the extract was then evaluated in terms of apoptosis and the cell cycle using flow cytometry. Mitochondrial membrane potential (MMP) was investigated using the fluorometric method, and expression levels of telomerase were studied using RT-PCR. R. canina extract exhibited a selective cytotoxic effect on WiDr cells compared with normal colon cells. The extract induced cell cycle arrest at the S phase and apoptosis via reduced MMP in WiDr cells. R. canina extract significantly repressed telomerase expressions at treatment times of 48 and 72 h in WiDr cells. Our results suggest that R. canina may have considerable potential for development as a novel natural product-based anticancer agent.

Synthesis and characterization of Rosa canina-mediated biogenic silver nanoparticles for anti-oxidant, antibacterial, antifungal, and DNA cleavage activities

“…Although it is difficult to combine all these properties, the selection of a material containing the majority is crucial to increase the success of the synthesis [16]. The extracts of the Rosa canina species of the Rosaceae family are potential phytochemicals for the reduction reaction due to the OH groups they contain [17, 18]. Saware et al.…”

Oxidative Stress Mediated Cytotoxicity, Cell Cycle Arrest, and Apoptosis Induced by Rosa damascena in Human Cervical Cancer HeLa Cells

“…The cytotoxicity and apoptosis responses through disruption of MMP in other cancer cells have also been investigated [ 59 ]. Numerous studies also revealed the apoptotic possessions of Rosa species, such Rosa canina extract in human colon cancer [ 60 ] and human lung and prostate cancer cells [ 61 ] through depolarization in mitochondrial membrane potential.…”

“…Our results are also supported by Khatib et al [ 63 ], who have shown that R. damascena oil induced cytotoxicity in gastric cancer cell line (MKN45) through apoptosis mechanism. Turan et al [ 60 ] have also demonstrated that Rosa canina extract exhibits concentration-dependent cytotoxic effects against human colon cancer cells by inducing apoptosis. Induction in cancer cell apoptosis has been extensively studied, making it an important indicator for the development of anticancer agents [ 55 ].…”

Role of Pentacyclic Triterpenoids in Chemoprevention and Anticancer Treatment: An Overview on Targets and Underling Mechanisms

“…Thus, during each round of cell division a portion of telomeres gets eroded and eventually it becomes non-functional. These telomeres are an attractive therapeutic target for cancer treatment [ 4 , 50 , 64 ]. Table 2 describes targets and mechanisms contributing anticancer activity of some pentacyclic triterpenoids.…”