Cytotoxic effect of Green synthesized silver nanoparticles using Melia azedarach against in vitro HeLa cell lines and lymphoma mice model

Sukirtha, Raman; Priyanka, Kandula Manasa; Antony, Jacob Joe; Kamalakkannan, Soundararajan; Thangam, Ramar; Gunasekaran, Palani; Krishnan, Muthukalingan; Achiraman, Shanmugam

doi:10.1016/j.procbio.2011.11.003

Cited by 314 publications

(141 citation statements)

References 30 publications

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“…DLS analysis showed the size distribution of the particles with an average hydrodynamic size of 73.55 nm and 78.87 nm for the Au-Ag BNPs leaf and flower, respectively, similar to the to the nanoparticles synthesized from the leaf extract of Melia azedarach [30]. As expected, the particle size obtained from TEM, SEM-EDX and DLS is marginally different due to the varying principles used for measurement.…”

Section: Dls and Zeta Potential Analysissupporting

confidence: 63%

Biosynthesis Of Bimetallic Au-Ag Nanoparticles Using Ocimum Basilicum (L.) With Antidiabetic And Antimicrobial Properties

Ramdas¹,

Mbatha²,

Gengan³

et al. 2015

Adv. Mater. Lett.

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This study was aimed at developing a simple, eco-friendly and cost effective green chemistry method for the synthesis of bimetallic Au-Ag nanoparticles using Ocimum basilicum aqueous leaf and flower extracts, respectively as the natural reducing agents. The successive reduction of chloroauric acid and silver nitrate led to the formation of Au-Ag nanoparticles within 10 min at room temperature, suggesting a higher reaction rate than chemical methods involved in the synthesis. Stable, spherical nanoparticles with well-defined dimensions of average size of 3-25 nm was confirmed by UV-Visible spectroscopy, TEM, SEM-EDX, DLS, and zeta potential, whilst, FTIR in combination with GC-MS analyzed the functional groups adhered to the surface of the nanoparticles. The colloidal suspension displayed enhanced antihyperglycemic activity at 69.97 ± 3.42% (leaf) against α-amylase (from porcine) and at 85.77 ± 5.82% (flower) against Bacillus stearothermophilus α-glucosidase than that of acarbose and their respective crude extracts. Furthermore, revealed good antibacterial activity against bacterial species Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Pseudomonas aeruginosa.

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Section: Dls and Zeta Potential Analysissupporting

confidence: 63%

Biosynthesis Of Bimetallic Au-Ag Nanoparticles Using Ocimum Basilicum (L.) With Antidiabetic And Antimicrobial Properties

Ramdas¹,

Mbatha²,

Gengan³

et al. 2015

Adv. Mater. Lett.

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“…Nanosilvers either chemically or biologically synthesized have unequalled properties, and have gained prominence because of their anticancer, antimicrobial, antibacterial, larvicidal, antioxidant antiparasitic, anticoagulant, thrombolytic and antifungal properties Jayaseelan et al 2011;Sukirtha et al 2012;Zahir and Rahuman 2012;Raliya et al 2015;Azeez et al 2016;Lateef et al 2016a, b, c, d;Ojo et al 2016). Due to these properties nanosilvers have been used in therapeutics, and applied in manufacturing of plastics, textile, healthcare products and electrical appliances (Gengan et al 2013;Schlich and Hund-Rinke 2015;Lateef et al 2016e).…”

Section: Introductionmentioning

confidence: 99%

Silver nanoparticles (AgNPs) biosynthesized using pod extract of Cola nitida enhances antioxidant activity and phytochemical composition of Amaranthus caudatus Linn

2017

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This study investigates the influence of different concentrations of AgNPs biologically synthesized using pod extract of Cola nitida on antioxidant activity, phenolic contents, flavonoid contents and compositions of Amaranthus caudatus L. AgNPs of 25, 50, 75, 100 and 150 ppm were utilized in growing A. caudatus while water was used as control. Delayed germination for two days was observed for A. caudatus grown with 150 ppm of AgNPs, while others showed no difference. There were 43.3, 38.7, 26.7 and 6.48% improvements in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) antioxidant activity of A. caudatus grown with 25, 50, 75 and 100 ppm of AgNPs, respectively, compared to control. Antioxidant activity of A. caudatus grown with AgNPs reduced with increase in the concentrations of AgNPs. A. caudatus grown with 50 ppm of AgNPs was the most potent with the least IC 50 of 0.67 mg/ml. Significant improvements obtained for phenolic and flavonoid contents grown with AgNPs were concentration dependent. Enhancements of 21.9, 68.19, and 1.98% in phenolic contents were achieved in treatments with 25, 50 and 75 ppm AgNPs, respectively, while 32.58, 35.80, and 7.20% improvement in flavonoids were obtained for 25, 50 and 100 ppm treatments, respectively. Kaempferol and quercetin were the most abundant flavonoids in A. caudatus treated with 50 ppm of AgNPs, showing the highest flavonoid composition. This further confirms A. caudatus grown with 50 ppm of AgNPs as the most potent. This study has shown that concentration-dependent AgNPs can be used to boost antioxidant activity and phytochemical contents of vegetables.

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“…According to Bansal et al (2011) biological synthesis of silver nanoparticles is the novel approach. Many previous researchers highlighted the green synthesis of silver nanoparticles (Vahabi et al 2011;Mondal et al 2014;Sukirtha et al 2012;Huang et al 2007). Green synthesis of silver nanoparticle has some advantages towards the reduction of metal ions and their stability (Narayanan and Sakthivel 2010).…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis of silver nanoparticles from Aloe vera leaf extract and antifungal activity against Rhizopus sp. and Aspergillus sp.

et al. 2014

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Silver nanoparticles are receiving increasing attention in the field of agriculture. This study aims at evaluating the antifungal properties of green synthesised silver nanoparticles (AgNPs) from Aloe vera leaf extract against two pathogenic fungus Rhizopus sp. and Aspergillus sp. Results revealed that synthesised nanoparticles showed strong absorption maximum at 400 nm corresponding to the surface plasmon resonance. The prepared nanoparticles were characterized by SEM, FT-IR and UVVis spectroscopy. From the scanning photograph it is clear that particles are heterogeneous in shape such as rectangular, triangular and spherical with uniform distribution. FT-IR study showed sharp absorption peaks at 1,631 and 3,433 cm -1 for amide and alcoholic hydroxide groups, respectively. On the other hand, synthesised silver nanoparticles showed highest antifungal activity against Aspergillus sp. than Rhizopus sp. by application of 100 lL of 1 M silver nanoparticles with maximum inhibition of the growth of fungal hyphae. However, microscopic observation revealed that synthesised nanoparticles caused detrimental effects on conidial germination along with other deformations such as structure of cell membrane and inhibited normal budding process of both the tested species. Therefore, it has been concluded that Aloe vera leaf extract origin silver nanoparticles have tremendous potentiality towards controlling pathogenic fungus. However, further research is needed to check the efficacy of sizedependent AgNPs on different species of fungus.

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Cytotoxic effect of Green synthesized silver nanoparticles using Melia azedarach against in vitro HeLa cell lines and lymphoma mice model

Cited by 314 publications

References 30 publications

Biosynthesis Of Bimetallic Au-Ag Nanoparticles Using Ocimum Basilicum (L.) With Antidiabetic And Antimicrobial Properties

Biosynthesis Of Bimetallic Au-Ag Nanoparticles Using Ocimum Basilicum (L.) With Antidiabetic And Antimicrobial Properties

Silver nanoparticles (AgNPs) biosynthesized using pod extract of Cola nitida enhances antioxidant activity and phytochemical composition of Amaranthus caudatus Linn

Biosynthesis of silver nanoparticles from Aloe vera leaf extract and antifungal activity against Rhizopus sp. and Aspergillus sp.

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