Biosynthesis of silver nanoparticles from Aloe vera leaf extract and antifungal activity against Rhizopus sp. and Aspergillus sp.

Abstract

Silver nanoparticles are receiving increasing attention in the field of agriculture. This study aims at evaluating the antifungal properties of green synthesised silver nanoparticles (AgNPs) from Aloe vera leaf extract against two pathogenic fungus Rhizopus sp. and Aspergillus sp. Results revealed that synthesised nanoparticles showed strong absorption maximum at 400 nm corresponding to the surface plasmon resonance. The prepared nanoparticles were characterized by SEM, FT-IR and UVVis spectroscopy. From the scanning photograph it is clear that particles are heterogeneous in shape such as rectangular, triangular and spherical with uniform distribution. FT-IR study showed sharp absorption peaks at 1,631 and 3,433 cm -1 for amide and alcoholic hydroxide groups, respectively. On the other hand, synthesised silver nanoparticles showed highest antifungal activity against Aspergillus sp. than Rhizopus sp. by application of 100 lL of 1 M silver nanoparticles with maximum inhibition of the growth of fungal hyphae. However, microscopic observation revealed that synthesised nanoparticles caused detrimental effects on conidial germination along with other deformations such as structure of cell membrane and inhibited normal budding process of both the tested species. Therefore, it has been concluded that Aloe vera leaf extract origin silver nanoparticles have tremendous potentiality towards controlling pathogenic fungus. However, further research is needed to check the efficacy of sizedependent AgNPs on different species of fungus.

Green synthesis of Mangifera indica silver nanoparticles and its analysis using Fourier transform infrared and scanning electron microscopy

“…The hydrogen bond and electrostatic bonds were responsible for AgNPs formation. [19] The results exhibit the same phenomenon as described by Ponarulselvam et al [20] CONCLUSION Nanotechnology finds extensive applications in nanomedicine, an emerging new field. The present study is an eco-friendly procedure for synthesizing AgNPs using M. indica leaves extract.…”

Characterization of silver nanoparticles by green synthesis method using Pedalium murex leaf extract and their antibacterial activity

“…Ag nanoparticles have been synthesised by different physical and chemical approaches viz., chemical reduction, microemulsion/reverse micelles, electrochemical reduction and photochemical reduction. Many biological approaches of green synthesis have been reported till date using plant leaf extracts from Alternanthera sessilis (Niraimathi et al 2013), Morinda citrifolia (Sathishkumar et al 2012), Mukia scabrella (Prabakar et al 2013), Iresine herbstii (Dipankar and Murugan 2012), Tribulus terrestris (Gopinath et al 2012), Azadirachta indica (Khan et al 2012), Cycas circinalis, Ficus amplissima, Commelina benghalensis, Lippia nodiflora (Johnson and Prabu 2015), Ocimum sanctum (Garima Singhal et al 2011) and Aloe vera (Medda et al 2014). Pedalium murex (P. murex) is a member of the sesame family, Pedaliaceae.…”

Electrospun PCL membranes incorporated with biosynthesized silver nanoparticles as antibacterial wound dressings

“…Biosynthesized silver nanoparticles were reported for their excellent antibacterial activity against wide range of microorganisms (Gnanadesigan et al 2012;Kathiraven et al 2014;Netala et al 2014;Sinha et al 2014;Kumar et al 2015;Kokila et al 2015;Roy et al 2014). Antifungal activity of biosynthesized silver nanoparticles is also well studied (Kumar et al 2013;Medda et al 2014).…”