Cytotoxic effect of curcumin on Giardia lamblia trophozoites

Pérez-Arriaga, L.; Mendoza-Magaña, María Luisa; Cortés-Zárate, Rafael; Corona‐Rivera, Alfredo; Bobadilla‐Morales, Lucina; Troyo-Sanromán, Rogelio; Ramírez-Herrera, Mario Alberto

doi:10.1016/j.actatropica.2006.03.005

Cited by 109 publications

(62 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As curcumin displays a very similar low micromolar activity against a number of protozoa in vitro, including Leishmania spp (Saleheen et al, 2002;Changtam et al, 2010a), Giardia lamblia (Pérez-Arriaga et al, 2006), Plasmodium falciparum (Mishra et al, 2008), and Trypanosoma brucei (Nose et al, 1998;Changtam et al, 2010a), the argument of limited specificity is compelling. In this respect, it is of interest to note that curcumin is known to alter membrane fluidity in an apoptosis-independent way (Jaruga et al, 1998;Atsumi et al, 2005;Hung et al, 2008;Ingolfsson et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Potent Trypanocidal Curcumin Analogs Bearing a Monoenone Linker Motif Act on Trypanosoma brucei by Forming an Adduct with Trypanothione

et al. 2014

View full text Add to dashboard Cite

We have previously reported that curcumin analogs with a C 7 linker bearing a C 4 -C 5 olefinic linker with a single keto group at C3 (enone linker) display midnanomolar activity against the bloodstream form of Trypanosoma brucei. However, no clear indication of their mechanism of action or superior antiparasitic activity relative to analogs with the original di-ketone curcumin linker was apparent. To further investigate their utility as antiparasitic agents, we compare the cellular effects of curcumin and the enone linker lead compound 1,7-bis(4-hydroxy-3-methoxyphenyl)hept-4-en-3-one (AS-HK014) here. An AS-HK014-resitant line, trypanosomes adapted to AS-HK014 (TA014), was developed by in vitro exposure to the drug. Metabolomic analysis revealed that exposure to AS-HK014, but not curcumin, rapidly depleted glutathione and trypanothione in the wild-type line, although almost all other metabolites were unchanged relative to control. In TA014 cells, thiol levels were similar to untreated wild-type cells and not significantly depleted by AS-HK014. Adducts of AS-HK014 with both glutathione and trypanothione were identified in AS-HK014-exposed wild-type cells and reproduced by chemical reaction. However, adduct accumulation in sensitive cells was much lower than in resistant cells. TA014 cells did not exhibit any changes in sequence or protein levels of glutathione synthetase and g-glutamylcysteine synthetase relative to wild-type cells. We conclude that monoenone curcuminoids have a different mode of action than curcumin, rapidly and specifically depleting thiol levels in trypanosomes by forming an adduct. This adduct may ultimately be responsible for the highly potent trypanocidal and antiparasitic activity of the monoenone curcuminoids.

show abstract

Section: Discussionmentioning

confidence: 99%

Potent Trypanocidal Curcumin Analogs Bearing a Monoenone Linker Motif Act on Trypanosoma brucei by Forming an Adduct with Trypanothione

et al. 2014

View full text Add to dashboard Cite

show abstract

“…In addition, curcumin possesses activities against bacteria, fungi, and protozoa. Cytotoxic and parasiticidal effects of curcumin on protozoan parasites have been demonstrated in cultures against Leishmania, Trypanosoma, Giardia, and Plasmodium falciparum (23,33,34,37,40). In vivo, curcumin also displayed potent activity against Plasmodium berghei, and it was synergistic with artemisinin (32,38).…”

mentioning

confidence: 90%

Cytotoxic Effect of Curcumin on Malaria Parasite Plasmodium falciparum : Inhibition of Histone Acetylation and Generation of Reactive Oxygen Species

Cui

Miao

Cui

2007

Antimicrob Agents Chemother

220

168

View full text Add to dashboard Cite

The emergence of multidrug-resistant parasites is a major concern for malaria control, and development of novel drugs is a high priority. Curcumin, a natural polyphenolic compound, possesses diverse pharmacological properties. Among its antiprotozoan activities, curcumin was potent against both chloroquine-sensitive and -resistant Plasmodium falciparum strains. Consistent with findings in mammalian cell lines, curcumin's prooxidant activity promoted the production in P. falciparum of reactive oxygen species (ROS), whose cytotoxic effect could be antagonized by coincubation with antioxidants and ROS scavengers. Curcumin treatment also resulted in damage of both mitochondrial and nuclear DNA, probably due to the elevation of intracellular ROS. Furthermore, we have demonstrated that curcumin inhibited the histone acetyltransferase (HAT) activity of the recombinant P. falciparum general control nonderepressed 5 (PfGCN5) in vitro and reduced nuclear HAT activity of the parasite in culture. Curcumin-induced hypoacetylation of histone H3 at K9 and K14, but not H4 at K5, K8, K12, and K16, suggested that curcumin caused specific inhibition of the PfGCN5 HAT. Taken together, these results indicated that at least the generation of ROS and down-regulation of PfGCN5 HAT activity accounted for curcumin's cytotoxicity for malaria parasites.

show abstract

“…Hence curcumin, used so copiously in many countries, could be a strong heavy metal chelator with little or no side effects. Curcumin, like other anti-oxidants, has a great ability to bind elements such as lead and other toxic elements and help to minimize the damage that can cause the survival of the element in the body [35,[49][50].…”

Section: Curcumin Attenuates Lead Toxicitymentioning

confidence: 99%

CURCUMIN ATTENUATES LEAD (Pb)–INDUCED NEUROBEHAVIORL AND NEUROBIOCHEMICAL DYSFUNCTION: A REVIEW

Abu-Taweel

2018

Int J Pharm Pharm Sci

View full text Add to dashboard Cite

Lead is one of the common chemical elements that is assigned the symbol Pb which came from the Latin Plumbum. Pb is widely used in the field of coating, refine and glaze ceramics and pottery. It is still used in the production of products like water pipes, cooking utensils and cooking utensils. In addition it is also used in insulation of building ceilings, cable coverage and military industries. Lead enter the environment from those uses and from the environment it enter into the living organisms. Lead accumulates in many humanorgans, but the brain is the target organ of lead accumulation. Neurotoxicity of lead is, one of lead toxicity, caused many symptoms. There are many behavioral and biochemical modifications induced by lead toxicity like learning and memory deficits, anxiety disorders, social and sexual behavior modifications and neurotransmitter system deficits. Curcumin is a bioactive natural phytochemical phenolic compound (diferuloylmethane) extracted from the rhizome of Curcuma longa. Most studies indicated the role of curcumin in reducing the damage of lead toxicity. In the current review, emphasis was based on the toxicity of lead and its effect on behavior and some neurotransmitters related to behavior. The effect of curcumin is improving the neurotoxicity and behavioral toxicity of lead.

show abstract

Cytotoxic effect of curcumin on Giardia lamblia trophozoites

Cited by 109 publications

References 37 publications

Potent Trypanocidal Curcumin Analogs Bearing a Monoenone Linker Motif Act on Trypanosoma brucei by Forming an Adduct with Trypanothione

Potent Trypanocidal Curcumin Analogs Bearing a Monoenone Linker Motif Act on Trypanosoma brucei by Forming an Adduct with Trypanothione

Cytotoxic Effect of Curcumin on Malaria Parasite Plasmodium falciparum : Inhibition of Histone Acetylation and Generation of Reactive Oxygen Species

CURCUMIN ATTENUATES LEAD (Pb)–INDUCED NEUROBEHAVIORL AND NEUROBIOCHEMICAL DYSFUNCTION: A REVIEW

Contact Info

Product

Resources

About