Cytotoxic Effect of Bee (<i>A. mellifera</i>) Venom on Cancer Cell Lines

Borojeni, Sima Khalilifard; Zolfagharian, Hossein; Babaie, Mahdi; Javadi, Iraj

doi:10.3831/kpi.2020.23.4.212

Cited by 15 publications

(17 citation statements)

References 29 publications

(33 reference statements)

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“…The amount of 50 µg of M. altirostris , M. corallinus , M. frontalis and M. carvalhoi crude venoms did not cause any cytotoxic effect on oocytes ( Figure 3 A), while 4 µg of Apis mellifera crude venom, used as a positive control, was enough to cause a prominent cytotoxic effect ( Figure 3 B). A. mellifera venom is already known in the literature to cause cytotoxic/cytolytic effects in different cells [ 41 , 42 , 43 , 44 , 45 , 46 ]. Interestingly, for the M. frontalis venom, we found an outward current around five times bigger when compared to the control ( Figure 3 A,C).…”

Section: Resultsmentioning

confidence: 99%

“…All the four venoms tested were not cytotoxic after the exposure of the oocytes using a saturating amount of venom, as shown in Figure 3 A. In contrast, we used, as a positive control, the venom from the Apis mellifera bee species, well known as having the activity of causing non-ion-selective pores in cell membranes, a cytotoxic/cytolytic effect [ 41 , 42 , 43 , 44 , 45 , 46 ]. In the electrophysiology experiments using the voltage ramp protocol, when a given venom is forming pores that are non-selective for ions and their conduction, typically two phenomena are observed: (1) a shift of the reversal potential to (wards) 0 mV, and (2) a significant increase in the chord conductance.…”

Section: Discussionmentioning

confidence: 99%

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Pharmacological Screening of Venoms from Five Brazilian Micrurus Species on Different Ion Channels

Kleiz-Ferreira

Bernaerts

Pinheiro-Júnior

et al. 2022

IJMS

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Coral snake venoms from the Micrurus genus are a natural library of components with multiple targets, yet are poorly explored. In Brazil, 34 Micrurus species are currently described, and just a few have been investigated for their venom activities. Micrurus venoms are composed mainly of phospholipases A2 and three-finger toxins, which are responsible for neuromuscular blockade—the main envenomation outcome in humans. Beyond these two major toxin families, minor components are also important for the global venom activity, including Kunitz-peptides, serine proteases, 5′ nucleotidases, among others. In the present study, we used the two-microelectrode voltage clamp technique to explore the crude venom activities of five different Micrurus species from the south and southeast of Brazil: M. altirostris, M. corallinus, M. frontalis, M. carvalhoi and M. decoratus. All five venoms induced full inhibition of the muscle-type α1β1δε nAChR with different levels of reversibility. We found M. altirostris and M. frontalis venoms acting as partial inhibitors of the neuronal-type α7 nAChR with an interesting subsequent potentiation after one washout. We discovered that M. altirostris and M. corallinus venoms modulate the α1β2 GABAAR. Interestingly, the screening on KV1.3 showed that all five Micrurus venoms act as inhibitors, being totally reversible after the washout. Since this activity seems to be conserved among different species, we hypothesized that the Micrurus venoms may rely on potassium channel inhibitory activity as an important feature of their envenomation strategy. Finally, tests on NaV1.2 and NaV1.4 showed that these channels do not seem to be targeted by Micrurus venoms. In summary, the venoms tested are multifunctional, each of them acting on at least two different types of targets.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Pharmacological Screening of Venoms from Five Brazilian Micrurus Species on Different Ion Channels

Kleiz-Ferreira

Bernaerts

Pinheiro-Júnior

et al. 2022

IJMS

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“…On the contrary, proteins such as p53, p21, and retinoblastoma (Rb) were upregulated in expression with the utility of BV, and the number of dead and apoptotic cells was significantly promoted ( Kim D. H. et al, 2020 ). It is worth noting that the main mode of death of HeLa cells after BV treatment is apoptosis, which causes severe cell membrane damage and cell shrinkage ( Borojeni et al, 2020 ), while MEL can show the effect of inhibiting HeLa cell proliferation and inducing apoptosis such as cell shrinkage and structural disorganization ( Zarrinnahad et al, 2018 ).…”

Section: Anticancer Effectsmentioning

confidence: 99%

Pharmacological effects and mechanisms of bee venom and its main components: Recent progress and perspective

Shi

Xie²,

Yang³

et al. 2022

Front. Pharmacol.

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Bee venom (BV), a type of defensive venom, has been confirmed to have favorable activities, such as anti-tumor, neuroprotective, anti-inflammatory, analgesic, anti-infectivity effects, etc. This study reviewed the recent progress on the pharmacological effects and mechanisms of BV and its main components against cancer, neurological disorders, inflammatory diseases, pain, microbial diseases, liver, kidney, lung and muscle injury, and other diseases in literature during the years 2018–2021. The related target proteins of BV and its main components against the diseases include Akt, mTOR, JNK, Wnt-5α, HIF-1α, NF-κB, JAK2, Nrf2, BDNF, Smad2/3, AMPK, and so on, which are referring to PI3K/Akt/mTOR, MAPK, Wnt/β-catenin, HIF-1α, NF-κB, JAK/STAT, Nrf2/HO-1, TrkB/CREB/BDNF, TGF-β/Smad2/3, and AMPK signaling pathways, etc. Further, with the reported targets, the potential effects and mechanisms on diseases were bioinformatically predicted via Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, disease ontology semantic and enrichment (DOSE) and protein-protein interaction (PPI) analyses. This review provides new insights into the therapeutic effects and mechanisms of BV and its main components on diseases.

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“…To assess the level of ROS generation, CLL the discovery of new compounds with anti-cancer effects is essential (Aufschnaiter et al, 2020;Borojeni et al, 2020). Today, venoms isolated from animal sources are of great importance for the treatment of cancer.…”

Section: Fraction B Increased Ros Generation In Cll Blymphocytesmentioning

confidence: 99%

Cytotoxicity Studies of the Crude venom and Fractions of Persian Gulf Snail (Conus Textile) on Chronic Lymphocytic Leukemia and Normal Lymphocytes

Salimi

Salehian

Aboutorabi³

et al. 2021

Asian Pac J Cancer Prev

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Background: Marine animals have been considered by many researchers due to their various pharmacological effects. One group of marine animals that have been studied is cone snails. The conotoxin obtained from these marine animals has various therapeutic effects. Methods: This study was designed to investigate the apoptotic effects of crude venom of Conus textile and its fractions (A and B) on chronic lymphocytic leukemia (CLL) cells. Accordingly, parameters such as cell viability, reactive oxygen species (ROS) level, collapse in mitochondrial membrane potential (MMP), lysosomal membrane damage and caspase-3 activation were evaluated. Results: The results showed that the crude venom (50, 100 and 200 µg/ml) from Conus textile and its fraction B (50, 100 and 200 µg/ml) significantly reduced viability in CLL B-lymphocyte. In addition, exposure of CLL B-lymphocyte to fraction B (50, 100 and 200 µg/ml) was associated with an increase in the level of ROS, the collapse of the MMP, damage to the lysosomal membrane, and activation of caspase-3. Conclusion: According to results, it was concluded that fraction B from crude venom of Conus textile causes selective toxicity on CLL B-lymphocyte with almost no effect on a normal lymphocyte. Furthermore, this venom fraction could be a promising candidate for induction of apoptosis in patients with CLL through the mitochondrial pathway.

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Cytotoxic Effect of Bee (A. mellifera) Venom on Cancer Cell Lines

Cited by 15 publications

References 29 publications

Pharmacological Screening of Venoms from Five Brazilian Micrurus Species on Different Ion Channels

Pharmacological Screening of Venoms from Five Brazilian Micrurus Species on Different Ion Channels

Pharmacological effects and mechanisms of bee venom and its main components: Recent progress and perspective

Cytotoxicity Studies of the Crude venom and Fractions of Persian Gulf Snail (Conus Textile) on Chronic Lymphocytic Leukemia and Normal Lymphocytes

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