Cytotoxic coumarins from the root ofAngelica dahurica

Abstract

Ten coumarins were isolated from the root of Angelica dahurica by repeated silica gel column chromatography. Their chemical structures were elucidated on the basic of physicochemical and spectroscopic data. Among them, oxypeucedanin hydrate acetonide (7) was isolated for the first time from this plant. Cytotoxicity of coumarins isolated were determined in vitro against L1210, HL-60, K562, and B16F10 tumor cell lines by MTT method. Pangelin (5) and oxypeucedanin hydrate acetonide (7) showed a potent cytotoxic activity with the IC50 values of 8.6 to 14.6 microg/mL against four kinds of tumor cell lines. Other compounds showed the moderate cytotoxic activity or no activity against the tumor cell lines.

Antioxidant activities of coumarins from Korean medicinal plants and their structure–activity relationships

“…Compounds 1-5 were identifi ed as scopoletin (Carpinella et al, 2005), aesculetin, fraxetin, fraxin and aesculin (Liu et al, 2005), respectively, by comparing their physicochemical and spectroscopic data with those published in the literature. Previously, the isolation and structural elucidation of 12 coumarins, 6-15 from A. dahurica (Thanh et al, 2004), 16 from E. daniellii (Yoo et al, 2002) and 17 from P. japonicum (Zheng et al, 2005) were reported. The chemical structures of these compounds (1-17) and four coumarins (18-21) obtained from Sigma Co. Ltd are presented in Fig.…”

“…The fraction F.6 was purifi ed on an LH-20 column (7.5 × 30 cm) and eluted with MeOH-H 2 O (4 : 1) to yield compounds 4 (2.5-2.8 L, 36 mg) and 5 (3.0-3.2 L, 21 mg Compounds 6-21. The isolation and structural elucidation of compounds 6-15 from the Korean medicinal plant Angelica dahurica were reported previously (Thanh et al, 2004), including isoimperatorin (6), osthol (7), imperatorin (8), psoralen (9), pangelin (10), bergapten (11), oxypeucedanin hydrate acetonide (12), oxypeucedanin (13), xanthotoxin (14), and oxypeucedanin hydrate (15). Two coumarins, isopimpinellin (16) and (+)-marmesin (17) were isolated from E. daniellii (Yoo et al, 2002) and P. japonicum (Zheng et al, 2005), respectively.…”

Evaluation of the effects of Angelicae dahuricae radix on the morphology and viability of mesenchymal stem cells

“…Limited studies were performed to evaluate the effects of Angelicae dahuricae radix on the cell viability in vitro and in vivo (14,15), and Angelicae dahuricae radix extract showed cytotoxicity toward cancer cells, including mouse lymphocytic leukemia cells, human promyelocytic leukemia cells, human myelogenous leukemia cells, and mouse melanoma cells (14). Coumarins were isolated from Angelicae dahuricae radix by silica gel column chromatography, and the half maximal inhibitory concentration (IC 50 ) values varied from 8.6 to 14.6 µg/ml cells.…”

A review of immunomodulators in the Indian traditional health care system

“…4). 92 Sapogenins Sapogenins, such as triterpenoid saponins (8) and diterpenes (9) exert a wide range of immunomodulatory activities. Examples are Gymnema sylvestre, Chlorophytum borivilianum, Boswellia spp.…”