Cytotoxic clerodane diterpenoids from the leaves of Premna tomentosa

Chin, Young‐Won; Jones, W.P.; Mi, Qiuwen; Rachman, Ismail; Riswan, Soedarsono; Kardono, Leonardus B.S.; Chai, Hee Byung; Farnsworth, Norman R.; Cordell, Geoffrey A.; Swanson, Steven M.; Cassady, John M.; Kinghorn, A. Douglas

doi:10.1016/j.phytochem.2006.04.015

Cited by 28 publications

(20 citation statements)

References 19 publications

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“…69 Premnones A–C ( 234–236 ) exhibited cytotoxic activity when evaluated against three human cancer cell lines (Lu1, LNCaP, and MCF-7), and one normal cell line (HUVEC) in the range of ED 50 of 0.7–7.0 μg mL −1 . 80 However, 234 was not active when evaluated in a follow-up in vivo hollow fiber assay at the highest dose tested (50 mg kg −1 ), using LNCaP, Lu1, and MCF-7 cells.…”

Section: Biological Activitiesmentioning

confidence: 96%

Clerodane diterpenes: sources, structures, and biological activities

2016

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The clerodane diterpenoids are a widespread class of secondary metabolites and have been found in several hundreds of plant species from various families and in organisms from other taxonomic groups. These substances have attracted interest in recent years due to their notable biological activities, particularly insect antifeedant properties. In addition, the major active clerodanes of Salvia divinorum can be used as novel opioid receptor probes, allowing greater insight into opioid receptor-mediated phenomena, as well as opening additional areas for chemical investigation. This article provides extensive coverage of naturally occurring clerodane diterpenes discovered from 1990 until 2015, and follows up on the 1992 review by Merritt and Ley in this same journal. The distribution, chemotaxonomic significance, chemical structures, and biological activities of clerodane diterpenes are summarized. In the cases where sufficient information is available, structure activity relationship (SAR) correlations and mode of action of active clerodanes have been presented.

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Section: Biological Activitiesmentioning

confidence: 96%

Clerodane diterpenes: sources, structures, and biological activities

2016

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“…From Premnua tomentosa Willd. (Verbenaceae), collected at Indonesia, three new neo-clerodane diterpene were isolated and evaluated against three human cancer cell lines and one normal cell line showing cytotoxic activity (Chin et al 2006). Three clerodane diterpenes possessing the a,bunsaturated carbonyl group at C-7-C-8-C-17 substructure were inactive as insect antifeedants, but they were cytotoxic toward an embryonic Drosophila melanogaster Henning (Diptera: Drosophilidae) cell line (Rasikari et al 2005).…”

Section: Neo-clerodane-diterpenesmentioning

confidence: 99%

Plant secondary metabolites from Argentinean semiarid lands: bioactivity against insects

Sosa

Tonn

2007

Phytochem Rev

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“…The focus in our laboratories has been the discovery of natural product inhibitors of cancer 22,24–28,30–34,49. However, the simplicity and versatility of the hollow fiber assay lends itself to other applications.…”

Section: Additional Applications Of the Hollow Fiber Assaymentioning

confidence: 99%

Use of the in Vivo Hollow Fiber Assay in Natural Products Anticancer Drug Discovery

Pezzuto

Farnsworth

et al. 2009

J. Nat. Prod.

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The in vivo hollow fiber assay was developed at the National Cancer Institute (NCI) to help bridge the gap between in vitro cell-based assays and human tumor models propagated in immunodeficient mice. The goal was to develop an intermediate assay that could help predict which compounds found active in the 60-cell line panel would be active in a subsequent xenograft system. This was necessary due to the high cost of the traditional xenograft assay in terms of number of animals required, time for assay completion, and financial commitment necessary. To address this problem, investigators of the NCI Developmental Therapeutics Program designed a method of propagating human cancer cells in inert hollow fibers with pores small enough to retain the cancer cells but large enough to permit entry of potential chemotherapeutic drugs, including large proteins and other important substances. Fibers containing proliferating cancer cells are transplanted into the peritoneum or under the skin, the host mice are treated with a test agent and the fibers are subsequently retrieved for analysis of viable cell mass. The assay has been successful in helping investigators from around the world, including our own research group, prioritize compounds active in vitro for further testing in the traditional xenograft system.

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Cytotoxic clerodane diterpenoids from the leaves of Premna tomentosa

Cited by 28 publications

References 19 publications

Clerodane diterpenes: sources, structures, and biological activities

Clerodane diterpenes: sources, structures, and biological activities

Plant secondary metabolites from Argentinean semiarid lands: bioactivity against insects

Use of the in Vivo Hollow Fiber Assay in Natural Products Anticancer Drug Discovery

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