Cytotoxic Assay of Semipolar Fraction Of Ethanolic Extract From Sugar Apple (Annona Squamosa L.) Stem Bark on T47D Cells

Abstract

Previous research has shown that some compounds in leaves and seeds of sugar apple have a cytotoxic activity. The aim of this research was to determine the cytotoxicity of semipolar fraction of ethanolic extract from sugar apple stem bark (Annona squamosa L.) on T47D cancer cells. The semipolar fraction of ethanolic extract from sugar apple stem bark was collected by fractionation using Vacuum Liquid Chromatography (VLC) with hexane:ethyl acetic (9:1, 8:2, 7:3, and 6:4) as mobile phase. Cytotoxicity from the fractions of five different concentration namely; 25, 50, 100, 150, and 250, µg/mL was measured by MTT assay. The potency of the cytotoxicity was defined by the ability of the fraction to inhibit the growth of T47D cells indicated by the value of IC50. Qualitative analysis of contained compounds in the fraction was done by Thin Layer Chromatography (TLC) method using silica gel F 254 as a stationary phase and hexane:ethyl acetic (7:3) as a mobile phase. UV 254 and 366 nm lamp also Dragendorff, citroboric, and FeCl3 spray reagents were used to visualize the spots of the secondary metabolites. The result proved that the semipolar fraction of ethanolic extract from sugar apple stem bark showed potential cytotoxicity on T47D cancer cells with IC50value of 70,77 µg/mL. Qualitative analysis showed that the fraction contained flavonoids and alkaloids which is presumably responsible for its cytotoxic activity.

Combination of Ketepeng Cina (Cassia alata Linn) And Lidah Buaya Buaya (Aloe vera) As Antibacterial Agent Against Staphylococcus aureus And Pseudomonas aeruginosa

“…Nevertheless, regarding the combination assay, the total amount of ZOI between the single treatment of CA and AV extracts in all groups was a lot higher than the combined extracts (Figure 3). Therefore, it implies that rather than a synergistic correlation between methanol extracts of CA and AV combination (Bliss, 1939), they seem to be antagonistic when the ratio of both extracts is not equal TLC chemical identification assay revealed positive results in various compound groups namely anthraquinones, flavonoids, and phenolics in both CA and AV, while the alkaloids group was only detected in CA extract (Table 2) (Wagner and Bladt, 1996;Muflihah et al, 2020). However, it is noteworthy that the development system for the TLC method needs to be optimized since not all the extracts at the bottom were eluted, which likely caused many other compound groups left undetected.…”

“…Aloe vera 6,0 ±0,0 6,0 ± 0,0 6,0 ± 0,0 6,0 ± 0,0 6,0 ± 0,0 6,0 ± 0,0 6,0 ± 0,0 Cassia alata extract, 5% Aloe vera extract, and the combined extract) were spotted at the lower line of the silica plate. Subsequently, the RFs of the spots were determined and visualized using ammonia vapor, FeCl3, SbCl3, KOH 10%, and Bouchardat reagents (Wagner and Bladt, 1996;Jangnga et al, 2018;Muflihah et al, 2020).…”

Phytochemical, Antioxidant and Antibacterial Evaluation of Melastoma malabathricum L.: an Indonesian Traditional Medicinal Plant

“…The compounds containing the flavonoid groups revealed yellow spots in the TLC plate. 30 The TLC profile of ethyl acetate fraction exhibited some spots with Rf value : 0.30, 0.50, and 0.70 with yellowish fluorescense under UV 254 nm, 366 nm and visible ray after sprayed by citroboric reagent. Dichloromethane fraction showed a few yellow spots on the plate.…”

Phytochemical screening, TLC profile and antioxidant activity of hydromethanolic extract of Centaurothamnus maximus leaves

“…The solvent system chloroform-methanol-formic acid (44:3.5:2.5) was the best system used for spot separation in the 80% methanol extract and it's fractions. The plates were sprayed with 5% FeCl3 reagents that identified flavonoid and polyphenol compounds and will turn to brown, grey and black colors, indicating flavonoids and polyphenols content in extracts [32]. The results of the detection with FeCl3 spray showed the present of phenol compounds.…”