Cytotoxic, Antioxidant and Antimicrobial Activity of Polygonum Chinensis Linn

Abstract

Polygonum chinensis linn belongs to the family Polygonaceae. The plant used in this study was shade dried and extracted with methanol by a cold maceration process. Qualitiave phytochemicals estimation showed the presence of the majority of compounds including terpinoids, flavonoids, tannins, saponins and glycosides. All the extracts showed moderate inhibition ranged from 12- 14 mm against both Gram-positive and Gram-negative strains and 00-21 mm against fungal trains. In the two-fold serial dilution method, the minimum inhibitory concentration of the extract ranges between 250 – >1000 µg/ml. The leaf extract of polygonum chinensis showed the potent activity with an IC50 value of 18.59 ± 0.356 µg/ml. In DPPH, the lipid peroxidation and hydrogen peroxide method, the leaf extract showed potent antioxidant activity. Root and stem extract showed moderate antioxidant activity. All the extracts showed moderate cytotoxicity to all the five cell lines Vero, A-549, L6, BRL, 3A and L-929 with CTC50 value ranging from 400-125 µg/ml. Short-term cytotoxicity studies were conducted for all the extracts against (Dalton Lymphocytes ascites) cells. The CTC50 value was found to be 435 µg/ml. Thus, the extracts of P. Chinensis may possess cytotoxic, antioxidant and antimicrobial activity.