Study of Antioxidant Activity of Formononetin by in Vitro Method

Vishnuvathan, V. J.; Lakshmi, K. S.; Srividya, A. R.

doi:10.22159/ijpps.2017v9i2.13964

Cited by 6 publications

(5 citation statements)

References 10 publications

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“…This finding suggests that the n-BuOH extract and compounds 1-4 and 6 are the best candidates to combat diseases associated with oxidative stress. In accordance with the results of this study, formononetin showed IC 50 values of 4.65, 9.48, 4.75, 5.0, and 4.27 μg/ml against DPPH, hydroxyl, hydrogen peroxide, nitric oxide, and superoxide radicals, respectively [43]. In addition, previous research has shown that luteolin had strong reactive oxygen species (ROS) scavenging effect against hydroxyl and superoxide radicals compared with that in the control [44].…”

Section: Discussionsupporting

confidence: 90%

“…A part of the EtOAc extract (65 g) of Trifolium baccarinii was subjected to silica gel column chromatography using n-hexane-EtOAc (100 : 0 ⟶ 0 : 100) followed by EtOAc-MeOH (90 : 10 ⟶ 80 : 20) gradient as an eluting system. Sixty-six fractions of 400 mL were collected and combined on the basis of their TLC profiles to give fourteen major fractions: A (1), B (2-6), C (7-10), D (11)(12)(13)(14), E (15)(16)(17)(18)(19)(20), F (21)(22)(23)(24)(25), G (26)(27)(28)(29)(30)(31), H (32)(33)(34), I (35)(36)(37)(38)(39)(40), J (41)(42)(43)(44)(45)(46), K (47)(48)(49)(50)(51)(52), L (53-58), M (59-60), and N (61-66). Purification of fraction G (8.0 g) on silica gel column chromatography with n-hexane-EtOAc (90 : 10) as an eluent yielded compounds 1 (27 mg) and 2 (35 mg).…”

Section: Isolation Of Compoundsmentioning

confidence: 99%

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Antimicrobial and Antioxidant Secondary Metabolites from Trifolium baccarinii Chiov. (Fabaceae) and Their Mechanisms of Antibacterial Action

Tsamo

Tamokou

Kengne

et al. 2021

BioMed Research International

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The treatment of infectious diseases with antimicrobial agents continues to present problems in modern-day medicine with many studies showing significant increase in the incidence of bacterial resistance to several antibiotics. The screening of antimicrobial activity of plant extracts and natural products has shown that medicinal plants are made up of a potential source of new anti-infective agents. The aim of this study was to evaluate the antimicrobial and antioxidant activities of extracts and compounds from the whole plant Trifolium baccarinii Chiov. and to determine their modes of antibacterial action. The plant extracts were prepared by maceration in organic solvents. The antimicrobial activities were evaluated using the broth microdilution method. The antioxidant activity was evaluated using the 2,2 ′ -diphenyl-1-picrylhydrazyl radical (DPPH) and 2,2 ′ -azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) assays. The mechanisms of antibacterial action were determined by lysis, salt tolerance assays, and antioxidant enzyme activities. The cytotoxic effect on the erythrocytes was determined by a spectrophotometric method. Biochanin A, formononetin, luteolin, luteolin-4 ′ -O-β-D-glucopyranoside, 4,7,2 ′ -trihydroxy-4 ′ -methoxyisoflavanol, sissotrin, 1-methyl-β-D-glucopyranoside, ononin, D-mannitol, and 3-O-β-D-glucuronopyranosylsoyasapogenol B were isolated from Trifolium baccarinii. The MeOH, EtOAc, and n-BuOH extracts as well as biochanin A, formononetin, luteolin, luteolin-4 ′ -O-β-D-glucopyranoside, 4,7,2 ′ -trihydroxy-4 ′ -methoxyisoflavanol, and sissotrin from Trifolium baccarinii displayed the highest antimicrobial and antioxidant activities. The MeOH extract and 4,7,2 ′ -trihydroxy-4 ′ -methoxyisoflavanol exhibited antibacterial activity through the bacteriolytic effect and reduction of the antioxidant defenses in the bacterial cells. The present study portrays Trifolium baccarinii as a potential natural source of antibacterial, antifungal, and antioxidant agents.

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Section: Discussionsupporting

confidence: 90%

Section: Isolation Of Compoundsmentioning

confidence: 99%

Antimicrobial and Antioxidant Secondary Metabolites from Trifolium baccarinii Chiov. (Fabaceae) and Their Mechanisms of Antibacterial Action

Tsamo

Tamokou

Kengne

et al. 2021

BioMed Research International

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“…From the results of the chromatographic analyses, it was possible to note the high concentration of formononetin and p-coumaric acid simultaneously in these samples, which may have directly influenced the ability to neutralize the DPPH free radical. In their in vitro study to determine the antioxidant potential of formononetin, Vishnuvathan et al [61] concluded that the ability of this substance to neutralize the DPPH radical increased in a concentration-dependent relationship. Shen et al [62] detected a similar relationship in an in vitro study of the antioxidant potential of p-coumaric acid against the DPPH radical.…”

Section: Quantification Of Compounds By Hplcmentioning

confidence: 99%

Extraction of Antioxidant Compounds from Brazilian Green Propolis Using Ultrasound-Assisted Associated with Low- and High-Pressure Extraction Methods

et al. 2023

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The demand for bee products has been growing, especially regarding their application in complementary medicine. Apis mellifera bees using Baccharis dracunculifolia D.C. (Asteraceae) as substrate produce green propolis. Among the examples of bioactivity of this matrix are antioxidant, antimicrobial, and antiviral actions. This work aimed to verify the impact of the experimental conditions applied in low- and high-pressure extractions of green propolis, using sonication (60 kHz) as pretreatment to determine the antioxidant profile in the extracts. Total flavonoid content (18.82 ± 1.15–50.47 ± 0.77 mgQE·g−1), total phenolic compounds (194.12 ± 3.40–439.05 ± 0.90 mgGAE·g−1) and antioxidant capacity by DPPH (33.86 ± 1.99–201.29 ± 0.31 µg.mL−1) of the twelve green propolis extracts were determined. By means of HPLC-DAD, it was possible to quantify nine of the fifteen compounds analyzed. The results highlighted formononetin (4.76 ± 0.16–14.80 ± 0.02 mg·g−1) and p-coumaric acid (<LQ—14.33 ± 0.01 mg·g−1) as majority compounds in the extracts. Based on the principal component analysis, it was possible to conclude that higher temperatures favored the release of antioxidant compounds; in contrast, they decreased the flavonoid content. Thus, the obtained results showed that samples pretreated with 50 °C associated with ultrasound displayed a better performance, which may support the elucidation of the use of these conditions.

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“…1A). These isoflavones, isolated from leguminous plants, have shown -glucosidase inhibition [13,14,[16][17][18], antioxidant [19],…”

Section: Introductionmentioning

confidence: 99%

Synthesis Of Alkoxy-Isoflavones As Potential α-Glucosidase Inhibitors

Aguila-Muñoz

Cervantes-Espinoza

Escalante

et al. 2022

Preprint

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The aim of the present study was to synthesize isoflavone-enaminones 3a-c and 7-alkoxy-isoflavones 4a-c, evaluate their inhibition of a-glucosidase, and analyze the bioisosteric effect of the presence versus absence of aromatic moieties in these benzopyran derivatives. All the test compounds exhibited a greater inhibition of a-glucosidase than the positive control acarbose. The series of isoflavones 3a-c and 4a-c showed a higher inhibitory activity (IC50 = 6.3 - 87.6 µM) than the parental 7-hydroxyisoflavones 2a-c (IC50 = 109.4 - 173.2 µM), suggesting that the attachment of a 4’-chloroacetophenone moiety to the 7-hydroxyl group of 2a-c is an efficient way to increase the inhibition of a-glucosidase. Furthermore, the aromatic moieties of the series of compounds 3 and 4 enhance the inhibitory activity by hydrophobic effects according to docking calculations.

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Study of Antioxidant Activity of Formononetin by in Vitro Method

Cited by 6 publications

References 10 publications

Antimicrobial and Antioxidant Secondary Metabolites from Trifolium baccarinii Chiov. (Fabaceae) and Their Mechanisms of Antibacterial Action

Antimicrobial and Antioxidant Secondary Metabolites from Trifolium baccarinii Chiov. (Fabaceae) and Their Mechanisms of Antibacterial Action

Extraction of Antioxidant Compounds from Brazilian Green Propolis Using Ultrasound-Assisted Associated with Low- and High-Pressure Extraction Methods

Synthesis Of Alkoxy-Isoflavones As Potential α-Glucosidase Inhibitors

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