Cytotoxic, Antimitotic, DNA Binding, Photocatalytic, H2O2 Sensing, and Antioxidant Properties of Biofabricated Silver Nanoparticles Using Leaf Extract of Bryophyllum pinnatum (Lam.) Oken

Abstract

Bryophyllum pinnatum is a perennial herb traditionally used in ethnomedicine. In the present report, silver nanoparticles (AgNPs) were synthesized using B. pinnatum leaf extract. BP-AgNPs were confirmed following UV-Vis spectroscopy with SPR peak at 412 nm and further characterized by FTIR, XRD, SEM-EDX, and TEM. Microscopic images confirmed the spherical shape and ~15 nm average size of nanostructures. BP-AgNPs were evaluated for photocatalytic degradation of hazardous dyes (methylene blue and Rhodamine-B) and showed their complete reduction within 100 and 110 min., respectively. BP-AgNPs have emerged as a unique SPR-based novel sensor for the detection of H2O2, which may deliver exciting prospects in clinical and industrial areas. DPPH and ABTS free radical scavenging activity were studied with respective IC50 values of 89 and 259 μg/mL. A strong intercalating interaction of CT-DNA with BP-AgNPs was investigated. Observed chromosomal abnormalities confirm the antimitotic potential of BP-AgNPs in the meristematic root tip. The cytotoxicity of BP-AgNPs against B16F10 (melanoma cell line) and A431 (squamous cell carcinoma cell line), was assessed with respective IC50 values of 59.5 and 96.61 μg/ml after 24 h of treatment. The presented green synthetic approach provides a novel and new door for environmental, industrial, and biomedical applications.

Silver Nanoparticles Synthesized from Abies alba and Pinus sylvestris Bark Extracts: Characterization, Antioxidant, Cytotoxic, and Antibacterial Effects

“…The EDX spectra of both A. alba and P. sylvestris bark extract-derived AgNPs ( Figure 4 A,B, respectively) revealed a pronounced signal for metallic Ag at 3 keV which confirmed the formation of AgNPs. Several other signals were registered for C, N, O, P, and Cl belonging to the compounds in bark extracts acting as capping agents [ 33 , 62 ].…”

“…The synthesis of AgNPs was monitored and optimized by recording the UV-Vis spectra of the reaction mixtures in the wavelength range of 350–800 nm [ 29 , 33 , 34 , 35 , 36 ] on a Specord 210 Plus spectrophotometer (Analytik Jena, Jena, Thuringia, Germany). In order to obtain the maximum yield of AgNPs, the synthesis was conducted at various parameters such as pH value of the reaction mixture (2, 4, 6, 8, and 10), AgNO 3 concentration (0.5, 1, 2, and 3 mM), ratio of aqueous bark extract and AgNO 3 (1:9, 2:8, 5:5, 7:3, and 9:1, v / v ), temperature (30, 40, 50, 60, 70, and 80 °C), and reaction time (10, 20, 30, 60, and 120 min).…”

Therapeutic potential of biogenic and optimized silver nanoparticles using Rubia cordifolia L. leaf extract

“…Mainly, the activity is attributed to the oxidative stress induced by AgNPs and induction of apoptosis via caspase-dependent pathway 20 . In a previous investigation, Bryophyllum pinnatum mediated BP-AgNPs also displayed cytotoxicity against the same cell lines 49 . However, after an incubation period of 24 h, the respective IC 50 values of BP-AgNPs against A431 and B16F10 were 59.5 and 96.6 µg/ml, almost double the IC 50 values of RC@AgNPs.…”

Leucophyllum frutescens mediated synthesis of silver and gold nanoparticles for catalytic dye degradation

“…Another study with onion peel as a reducing agent synthesized AgNPs at 90°C which were spherical in shape with 12.5 nm particle size ( Abdullah et al, 2021 ). Earlier studies on phytofabrication of AgNPs using Bryophyllum pinnatum leaf extract also showed predominance of similar spherical shaped particles with an average size of ∼15 nm ( Chandraker et al, 2021a ). However, anisotropic polydispersed AuNPs were synthesized by LFLE which is in close agreement with triangular, pentagonal, and hexagonal shaped AuNPs synthesized using extracts from various plants such as blackberry, blueberry, pomegranate, and turmeric ( Nadagouda et al, 2014 ; Castillo-Henríquez et al, 2020 ).…”