Cytotoxic and N-Acetyltransferase Inhibitory Meroterpenoids from Ganoderma cochlear

Abstract

Seven compounds, including two pairs of new meroterpenoids, (+)- and (−)-gancochlearol C (1), (+)- and (−)-cochlearoid Q (3), and a new meroterpenoid gancochlearol D (2), together with four known meroterpenoids were isolated from the aqueous EtOH extract of the fruiting bodies of Ganoderma cochlear. Their structures were determined by spectroscopic data. The isolated compounds were evaluated for their cytotoxic activity against three human lung cancer cells (H1975, PC9, A549) and N-acetyltransferase inhibitory property. The results show that (+)-gancochlearol C could inhibit N-acetyltransferase with an IC50 value of 5.29 μM. In addition, ganomycin F was found to show moderate activity against the H1975 human lung cancer cell line, with an IC50 value of 19.47 μM.

Meroterpenoid Dimers from Ganoderma Mushrooms and Their Biological Activities Against Triple Negative Breast Cancer Cells

“…The substructure of part A in 6 is similar to that of part A in 1 as they have very similar NMR data. The substructure of part B is very similar to ganomycin F ( Cheng et al, 2018 ). The difference is that the 3-position of the benzene ring in part A is connected to the other additional substructures, which is a hydrogen atom in ganomycin F. The HMBC correlation from H-6′ to C-10 suggests that C-10 is connected to C-5′.…”

“…The analysis of 2D NMR spectra (See Supplementary Figures S30–S33 ) of dimercochlearlactone C ( 3 ) reveals that the structure of part B is similar to that of ganomycin F ( Cheng et al, 2018 ). Thus, there are four possibilities for the connection between part A and part B of dimercochlearlactone C ( 3 ), C-4-O-C-1′, C-4-O-C-4′, C-9-O-C-1′, and C-9-O-C-4′.…”

Meroterpenoids from the Fungus Ganoderma sinensis and First Absolute Configuration Clarification of Zizhine H

“…Thereafter, increasing numbers of meroterpenoids were characterized by us. Our previous study disclosed that meroterpenoids are also in vitro active toward cancer cells [23][24][25]. Whether meroterpenoids resulting from the present study are also potent against cancer cells needs examination.…”

Biological activities of meroterpenoids isolated from different sources

“…From fruiting bodies of Ganoderma cochlear (Blume & T. Nees) Bres., (±)-gancochlearols A and B were isolated and reported to have potent COX-2 inhibitory activity (Qin et al, 2018c). Similarly, (±)-spirocochlealactones A-C, new spiro meroterpenoid podimeric enantiomers, and ganodilactone, with IC 50 values of 1.29-3.63 μM showed potent COX-2 inhibitory activity against lung, immortalized myelogenous leukemia, and hepatic cell lines (Qin F.-Y. et al, 2018).…”

“…et al, 2019 ). Two more meroterpenoids, gancochlearol D and ganomycin F, have been reported for their cytotoxic effect against lung cancer cells of various types, with ganomycin F being more potent than gancochlearol D ( Cheng et al, 2018 ). Spirocochlealactones A–C also have a potential cytotoxic effect against A549, Huh-7, and K562 cancer cell lines ( Qin F.-Y.…”