Cytotoxic and mutagenic effects of iodine-131 and radioprotection of acerola (Malpighia glabra L.) and beta-carotene in vitro

Abstract

ABSTRACT. The radioisotope iodine-131 [131 I] can damage DNA. One way to prevent this is to increase the amount of antioxidants via dietary consumption. The goal of this study was to evaluate the radioprotective effect of fresh acerola pulp and synthetic beta-carotene in Rattus norvegicus hepatoma cells (HTC)

In vivo antimutagenic effects of the Barbados cherry fruit (Malpighia glabra Linnaeus) in a chromosomal aberration assay

“…BCF reduced this percentage by 88.26% (SIM), 87.32% (PRE), and 86.85% (POST). Almeida et al (2013) corroborated the results of the present study. They demonstrated that the juice of fresh BC reduced the mean number of iodine-131 radiation-induced micronuclei in hepatoma cells of R. norvegicus in vitro by 50%.…”

“…In this sense, the antimutagenic activity of BC juice, which was evaluated in the present study, may be due to VC as well as to a mixture of chemical compounds that are present in the fruit, such as phenolic compounds, anthocyanins, carotenoids, and flavonoids (Almeida et al, 2013;Düsman et al, 2014), together with vitamins A, B1, B2, and B3, as well as calcium, iron, sodium, and phosphorus.…”

“…Additionally, the two pulps tested (fresh and frozen) had similar effects on the mitotic index in the antimutagenic treatment groups (SIM, PRE, and POST). Negative results for BC cytotoxicity were also found by Düsman et al (2012), in an in vivo chromosomal aberration test using bone marrow cells of Wistar rats; by Almeida et al (2013), in an in vitro micronucleus test using hepatoma cells of R. norvegicus; and by Horta et al (2016), in an in vivo micronucleus test with the bone marrow cells of mice. Motohashi et al (2004) also found that extracts of Barbados cherry fruit were not cytotoxic to normal human periodontal ligament fibroblasts or gingival fibroblasts.…”

Radioprotective effect of the Barbados Cherry (Malpighia glabra L.) against radiopharmaceutical Iodine-131 in Wistar rats in vivo

“…Abnormalities decreased by approximately 72% (simultaneous treatment and post-treatment) and 70% (pre-treatment). These percentages were as high and higher than those found by Almeida [27], wherein in natura Barbados Cherry juice reduced the average number of micronuclei induced by 131I in Rattus norvegicus hepatoma cells by 50%. Vitamin C, a major antioxidant component of the Barbados Cherry (1960.02 ± 2.32 mg/100 g of sample – Table 2), was assessed by Narra et al [28] at a concentration of 1.5 μg and showed a radioprotective effect when administered 4 hours before a 131I radioisotope dose of 0.85 μCi (pre-treatment) in mouse spermatocytes.…”

“…Almeida et al [27] also observed the absence of cytotoxic effects of Barbados Cherry treatments for 24 hours alone or together with 131I by the micronucleus test with in vitro hepatoma cells of Rattus norvegicus . Similarly, negative results for the cytotoxicity of this fruit were found by Düsman et al [14] by a chromosome aberration test with bone marrow cells of rats in vivo .…”