Cytotoxic and genotoxic responses of human lung cells to combustion smoke particles of Miscanthus straw, softwood and beech wood chips

Arif, Ali; Maschowski, Christoph; Garra, Patxi; Garcia-Käufer, Manuel; Petithory, Tatiana; Trouvé, Gwenaëlle; Dieterlen, Alain; Mersch-Sundermann, Volker; Khanaqa, Polla Azad; Nazarenko, Irina; Gminski, Richard; Gieré, Reto

doi:10.1016/j.atmosenv.2017.05.019

Cited by 28 publications

(27 citation statements)

References 71 publications

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“…In vitro studies have demonstrated that biological effects induced by water-soluble extracts from biomass burning particles can generate ROS [46] and induce DNA damage [15,23,25,46]. In our study, the acute cytotoxicity of the wood tar extracts (at concentrations of 0.2 and 2 mg/ml) was investigated by using the permeable dye PI, a WST-1 assay and electron microscopy with BEAS2B cells.…”

Section: Cytotoxicity Of Wood Tar To Lung Epithelial Cellsmentioning

confidence: 96%

“…Toxicological evaluations of ambient PM have been extensively conducted, whereas the toxicology and mechanisms of WSPs and related components have been poorly defined. Recent studies on WSP exposure have suggested that WSPs enhance inflammation and oxidative stress responses [4,[23][24][25][26][27]. The oxidative stress paradigm suggests that low levels of ROS/ oxidative stress can induce antioxidant induction to restore redox homeostasis.…”

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confidence: 99%

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Mechanisms of lung toxicity induced by biomass burning aerosols

Pardo

et al. 2020

Part Fibre Toxicol

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Background: Carbonaceous aerosols emitted from indoor and outdoor biomass burning are major risk factors contributing to the global burden of disease. Wood tar aerosols, namely, tar ball particles, compose a substantial fraction of carbonaceous emissions, especially from biomass smoldering. However, their health-related impacts and toxicity are still not well known. This study investigated the toxicity of the water-soluble fraction of pyrolyzed wood tar aerosols in exposed mice and lung epithelial cells.Results: Mice exposed to water-soluble wood tar aerosols showed increased inflammatory and oxidative stress responses. Bronchial epithelial cells exposed to the same water-soluble wood tar aerosols showed increased cell death with apoptotic characteristics. Alterations in oxidative status, including changes in reactive oxygen species (ROS) levels and reductions in the expression of antioxidant genes related to the transcription factor Nrf2, were observed and were confirmed by increased levels of MDA, a lipid peroxidation adduct. Damage to mitochondria was observed as an early event responsible for the aforementioned changes. Conclusions:The toxicity and health effect-related mechanisms of water-soluble wood tar were investigated for the first time in the context of biomass burning. Wood tar particles may account for major responses such as cell death, oxidative stress, supression of protection mechnaisms and mitochondrial damaged cause by expsoure to biomass burning aerosols.

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Section: Cytotoxicity Of Wood Tar To Lung Epithelial Cellsmentioning

confidence: 96%

mentioning

confidence: 99%

Mechanisms of lung toxicity induced by biomass burning aerosols

Pardo

et al. 2020

Part Fibre Toxicol

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“…It is more flexible than other statistical models and has greater advantages in dealing with the complex non-linear relationship between independent variables and strain variables. Due to the daily number of ASOM visits was sparse and typically followed a Poisson distribution [9], the core analysis was a GAM with log link and Poisson error that accounted for smooth fluctuations in daily number of consultations for ASOM patient. A detailed introduction of the GAM has been previously described [10].…”

Section: Generalized Additive Modelmentioning

confidence: 99%

“…For seasonal analysis, seasonality was differentiated on the basis of heating/non-heating periods. This study divides the seasons according to the meteorological characteristics of Northwest China, it is defined that spring is from February to April(2-5), the summer is from May to August(5-8), the autumn is from September to November (9)(10)(11), and the winter is from December to next year January(12 − 2).…”

Section: Generalized Additive Modelmentioning

confidence: 99%

“…Although there is convincing evidence for an association between environmental tobacco smoke exposure and OM [9], the relationship between ASOM and ambient air pollution exposure is not yet established, and the impact of meteorological factors on ASOM is rarely reported. As one of the most common diseases in children, ASOM mostly secondary to respiratory tract infections, and is the most common cause of antibiotic use in people.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of the Association between environmental-meteorological factors and Acute Suppurative Otitis Media: Further Epidemiological Evidence from Lanzhou, Northwestern China

Wang¹,

Xu²,

Tian³

et al. 2020

Preprint

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Objective To investigate the possible associations, lag effects and pathogenic characteristics of urban air quality on acute suppurative otitis media (ASOM）in Lanzhou, a city in Northwestern China. Methods SPSS software was used to analyze Spearman correlation between ambient environment-meteorological factors and the daily number of patient ASOM visits. The GAM of time series was used to analyze the exposure -response curve, and R software was used to calculate RR.Using above results to discuss the relationship of air pollutions , meteorological factors and morbidity of ASOM. Results The number of patients with ASOM in 2014, 2015 and 2016 were 916, 875 and 1023 respectively. There was no significant difference in three years (P=0.85). The annual visits peaked in December, the lowest in August, followed by February, Summer was less than the other three seasons. The morbidity of ASOM had a largest peak within 14 years old (53.84%), and the number of male patients 1514 (53.6%) is 1.16 times that of female patients 1309 (46.4%). Spearman correlation analysis showed that the correlation between air pollutions and environmental variables. As for single-day lag effects , there were positive correlations between PM 2.5 , PM 10 , CO, NO 2 , SO 2 and the daily number of ASOM visits, but the correlation between O 3 and ASOM did not pass the significance test . As for multi-day lag effects, PM 2.5 , PM 10 , CO, NO 2 had lagging effects on the daily number of ASOM visits, but the correlation between SO 2 and O 3 did not pass the significant test. There was a positive correlation between ATM and the daily number of patient ASOM visits, and negative correlation between T and the daily number of patient ASOM visits ,while the correlation between W, RH and ASOM was weak. Conclusions The incidence of ASOM has obvious age and seasonal characteristics. There may be some correlation between environmental-meteorological factors and the incidence of ASOM. Therefore, it is of great significance to strengthen environmental control and public health strategy in order to reduce and prevent the occurrence of ASOM diseases.

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Toxicity of particles derived from combustion of Ethiopian traditional biomass fuels in human bronchial and macrophage-like cells

McCarrick,

Delaval,

Dauter

et al. 2024

Arch Toxicol

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The combustion of traditional fuels in low-income countries, including those in sub-Saharan Africa, leads to extensive indoor particle exposure. Yet, the related health consequences in this context are understudied. This study aimed to evaluate the in vitro toxicity of combustion-derived particles relevant for Sub-Saharan household environments. Particles (< 2.5 µm) were collected using a high-volume sampler during combustion of traditional Ethiopian biomass fuels: cow dung, eucalyptus wood and eucalyptus charcoal. Diesel exhaust particles (DEP, NIST 2975) served as reference particles. The highest levels of particle-bound polycyclic aromatic hydrocarbons (PAHs) were found in wood (3219 ng/mg), followed by dung (618 ng/mg), charcoal (136 ng/mg) and DEP (118 ng/mg) (GC–MS). BEAS-2B bronchial epithelial cells and THP-1 derived macrophages were exposed to particle suspensions (1–150 µg/mL) for 24 h. All particles induced concentration-dependent genotoxicity (comet assay) but no pro-inflammatory cytokine release in epithelial cells, whereas dung and wood particles also induced concentration-dependent cytotoxicity (Alamar Blue). Only wood particles induced concentration-dependent cytotoxicity and genotoxicity in macrophage-like cells, while dung particles were unique at increasing secretion of pro-inflammatory cytokines (IL-6, IL-8, TNF-α). In summary, particles derived from combustion of less energy dense fuels like dung and wood had a higher PAH content and were more cytotoxic in epithelial cells. In addition, the least energy dense and cheapest fuel, dung, also induced pro-inflammatory effects in macrophage-like cells. These findings highlight the influence of fuel type on the toxic profile of the emitted particles and warrant further research to understand and mitigate health effects of indoor air pollution.

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Cytotoxic and genotoxic responses of human lung cells to combustion smoke particles of Miscanthus straw, softwood and beech wood chips

Cited by 28 publications

References 71 publications

Mechanisms of lung toxicity induced by biomass burning aerosols

Mechanisms of lung toxicity induced by biomass burning aerosols

Analysis of the Association between environmental-meteorological factors and Acute Suppurative Otitis Media: Further Epidemiological Evidence from Lanzhou, Northwestern China

Toxicity of particles derived from combustion of Ethiopian traditional biomass fuels in human bronchial and macrophage-like cells

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