Cytotoxic and apoptotic effects of Ebenus boissieri Barbey on human lung cancer cell line A549

Aydemir, Esra; Şimşek, Ece; İmir, Nilüfer Gülmen; Göktürk, Ramazan Süleyman; Yeşilada, Erdem; Fışkın, Kayahan

doi:10.4103/0973-1296.157679

Cited by 14 publications

(10 citation statements)

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“…The World Health Organization estimates that 4 billion people (i.e., 80% of the World's population) use herbal medicines in some aspects of primary healthcare, and there is a growing tendency to “Go Natural.”;[ 1 2 ] It is well established that plants have always been a useful source, for the occurrence of anticancer compounds. [ 3 4 5 ] Approximately 60% of the currently used anticancer chemotherapeutic drugs (vinblastin, vincristine) are derived from plant resource. [ 6 7 ] Although most of the plants used in the traditional medicine have been identified and their applications are well-documented, the anticancer efficacy of many plants is yet to be verified.…”

Section: Introductionmentioning

confidence: 99%

Evaluating the anticancer potential of ethanolic gall extract of Terminalia chebula (Gaertn.) Retz. (combretaceae)

et al. 2016

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Plants have been an important source for discovery of anticancer compounds. With the current decline in the number of new molecular entities from the pharmaceutical industry, novel anticancer agents are being sought from traditional medicines; therefore the anticancer efficacy of many plants that are used in traditional medicine is yet to be verified. The objective of the study was to evaluate the cytotoxic potential of ethanolic leaf gall extract of Terminalia chebula are evaluated against buffalo rat liver 3A, MCF-7 (Human mammary gland adenocarcinoma) and A-549 (Human lung cancer) cell lines. The cytotoxic effect of the ethanolic extract was evaluated by MTT assay. The extract was potent and effective in inducing cytotoxic effects in all the cell lines with an IC50 value of 305.18 ± 1.7 μg/mL, 643.13 ± 4.2 μg/mL, and 208.16 ± 3.7 μ/mL, respectively. The extract was more effective against A549 cell lines when compared to others. The presences of phenolics, triterpenoids, and flavonoids were identified in the extract. The extract showed total phenolic and flavonoid content of 478 ± 2.2 mg of gallic acid equivalent/g d.w and 538 ± 1.4 mg of quercetinequivalent/g d.w, respectively. This higher content of total phenolics and flavonoids found in the ethanolic extract was directly associated to higher cytotoxicity activity.Conclusion:The ethanolic leaf gall extract of T. chebula showed effective cytotoxic activities; which might be attributed to the phenolics/flavonoids present in higher concentration. Future work will be interesting to know the chemical composition of the extract and also better understand the mechanism of action of the constituents present in the extract to develop it as drug for therapeutic application.SUMMARY The present investigation establishes the anticancer activities of T. chebula leaf gall extracts on BRL3A, MCF-7, and A-549 cells. Presumably, these activities could be attributed in part to the phenolics/flavanoids features of the extract that has been demonstrated to act as cytotoxic agents. The experimental evidence obtained in the laboratory model could provide a rationale for the traditional use of plant as a source of easily available effective anticancer agents to the people, particularly in developing countries.

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Section: Introductionmentioning

confidence: 99%

Evaluating the anticancer potential of ethanolic gall extract of Terminalia chebula (Gaertn.) Retz. (combretaceae)

et al. 2016

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“…The dose range (0.01-1000 μg/mL) was determined for both plant extracts and Doxorubicin-HCl in our previous study. [ 19 ] As shown in Figure 1A and 1B , root extract had more inhibitory activity on proliferation of 293T than HeLa cells. In HeLa cells, the IC 50 of aerial and root extract were approximately 92.35 ± 0.39, 49.82 ± 0.27, 28.03 ± 0.12 μg/mL and 250 ± 5.83, 100.16 ± 4.28, 41.04 ± 1.09 for 24, 48, and 72 h, respectively.…”

Section: Resultsmentioning

confidence: 97%

Caspase-mediated apoptotic effects of Ebenus boissieri barbey extracts on human cervical cancer cell line hela

Şimşek¹,

İmir²,

Aydemir³

et al. 2017

Phcog Mag

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Background:Ebenus boissieri Barbey is an Antalya, Turkey-endemic plant belonging to Fabaceae family. The aerial parts and the roots of E. boissieri Barbey were used in this study.Objective:In the present study, we have examined the apoptotic effects of hydroalcoholic extracts of E. boissieri Barbey in human cervical cancer cell line HeLa.Materials and Methods:To determine the cytotoxic effect, cells were treated with various concentrations of extracts for 24, 48, and 72 h incubation periods. Cytotoxic effects were examined by Cell Titer 96 aqueous nonradioactive cell proliferation assay and the results were corrected by live/dead viability/cytotoxicity assay and trypan blue exclusion assay. Apoptotic effects were studied with multicaspase kit. Tumor necrosis factor-alpha (TNF-α) and interferon gamma (IFN-γ) release were also measured by enzyme-linked immunosorbent assay.Results:According to the results, E. boissieri Barbey extract caused significant increase in caspase levels. Thus, we suggest that the extract induces cells to undergo apoptosis. Especially, there was a sharp induction in caspase-3 activity. Levels of both TNF-α and IFN-γ in extract-treated groups were significantly and dose dependently exalted as compared to their relative controls.Conclusion:The effects of the extract on caspase-3, TNF-α, and IFN-γ levels mediate the plausible mechanism of apoptosis induction in HeLa. To the best of our knowledge, this is the first report indicating any pharmacological properties of E. Boissieri on HeLa cells.SUMMARY HeLa cell viability was reduced in dose-dependent manner for 72 h with an IC50 of approximate 28.03 μg/mL for aerial and 41.02 μg/mL for rootHeLa cells, exposure to the aerial extract led to 1.9, 3.8, 1.2, 2.4, and 3.45 fold induction of all caspases activities (-2, -3, -6, -8, and -9, respectively)Both 30 μg/mL of aerial and 45 μg/mL of root extracts allowed the production of anticancer cytokines (TNFalpha; IFNgamma) in HeLa cell culture supernatants. Abbreviations used: Tumor necrosis factor-alpha (TNF-α); Interferon gamma (IFN-γ); 3-(4, 5 dimethylthiazol-2-yl)-5-(3- carboxymethoxy-phenyl)-2-(4-sulfonyl)-2H-tetrazolium (MTS); Phosphate-Buffered Saline (PBS); Fetal Bovine Serum (FBS); para-Nitroanilin pNA; Enzyme-Linked ImmunoSorbent Assay (ELISA); Sodium Dodesyl sulphate –Polyacrilamide gel electrophoresis (SDS-PAGE); Tris-Buffered Saline (TBS); Hydocloric acid (HCl); Standart Error of Mean (SEM); National Cancer Institute (NCI); half maximal inhibitory concentration (IC50)

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“…In support of our study on medicinal plants against cancer, a recent research has reported that Fabaceae family members possess preventive and therapeutic potentials against various types of cancers. They demonstrated that the hydroalcoholic extracts of Ebenus boissieri barbey has anti-apoptotic and anti-carcinogenic activity against lung cancer cell line A549 (Aydemir et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Antioxidant and Anti-Proliferative Activity of Calamintha officinalis Extract on Breast Cancer Cell Line MCF-7

Moattar¹,

Sariri²,

Giahi³

et al. 2015

J. of Biological Sciences

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The aim of this study was evaluation of antioxidant and anti-proliferative activities of the Calamintha officinalis Moench (COM). Calamintha officinalis is a genus of aromatic herbs, which is native in north parts of Iran. Some of its pharmaceutical profits are already known, however, a scientific evidence seems necessary before any proposal for its pharmaceutical arrangements. Total antioxidant activity of the plant extract was assayed by spectrophotometric methods. Folin-ciocalteu reagent assay was used to evaluate the phenolic contents of extract. A zymogram assay of peroxidase (POX) was performed to observe its activity. Antiproliferative activity of the plant extract was examined on human breast cancer cell line (MCF-7) using [3-(4, 5-dimethylthiazolyl)-2, 5-diphenyl-tetrazolium bromide (MTT) method. The results showed a considerable antioxidant activity and a high reducing power. On the other hand, a reasonable antiproliferative role played by the extract, the POX in this plant is composed of two isozymes. The results indicated that COM can be regarded as a suitable candidate for designing anticancer pharmaceutical preparations.

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Cytotoxic and apoptotic effects of Ebenus boissieri Barbey on human lung cancer cell line A549

Cited by 14 publications

References 35 publications

Evaluating the anticancer potential of ethanolic gall extract of Terminalia chebula (Gaertn.) Retz. (combretaceae)

Evaluating the anticancer potential of ethanolic gall extract of Terminalia chebula (Gaertn.) Retz. (combretaceae)

Caspase-mediated apoptotic effects of Ebenus boissieri barbey extracts on human cervical cancer cell line hela

Antioxidant and Anti-Proliferative Activity of Calamintha officinalis Extract on Breast Cancer Cell Line MCF-7

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