Cytotoxic and Apoptotic Effects of Extracts of Artemisia ciniformis Krasch. &amp; Popov ex Poljakov on K562 and HL-60 Cell Lines

Tayarani‐Najaran, Zahra; Hajian, Zahra; Mojarrab, Mahdi; Emami, Seyed Ahmad

doi:10.7314/apjcp.2014.15.17.7055

Cited by 20 publications

(18 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Transgenic plants were analyzed to consider their taxadiene production. Results demonstrate the possibility of genetic engineering of the taxane biosynthetic pathway in Artemisia annua L. for the production of taxadiene (42, 43). …”

Section: Resultsmentioning

confidence: 84%

A Systematic Review of Anti-malarial Properties, Immunosuppressive Properties, Anti-inflammatory Properties, and Anti-cancer Properties of Artemisia Annua

Alesaeidi¹,

Miraj²

2016

Electron physician

View full text Add to dashboard Cite

Artemisia annua belongs to the asteraceae family, indigenous to the mild climate of Asia. The aim of this study was to overview its anti-malarial properties, immunosuppressive properties, anti-inflammatory properties and anti-cancer properties. This systematic review was carried out by searching studies in PubMed, Medline, Web of Science, and IranMedex databases. The initial search strategy identified approximately ninety eight references. In this study, forty six studies were accepted for further screening and met all of our inclusion. The search terms were “Artemisia annua”, “therapeutic properties”, “and pharmacological effects”. Artemisia annua is commonly used for its anti-malarial, immunosuppressive anti-inflammatory properties. Artemisia annua contributes to the treatment of various diseases such as diabetes, heart diseases, arthritis and eczema and possesses various effects such as antibacterial, antioxidant, anticoccidial, and antiviral effects. Furthermore, it was said to be good for cancer treatment. In this study, anti-malarial, immunosuppressive, anti-inflammatory properties of this plant are presented using published articles in scientific sites.

show abstract

Section: Resultsmentioning

confidence: 84%

A Systematic Review of Anti-malarial Properties, Immunosuppressive Properties, Anti-inflammatory Properties, and Anti-cancer Properties of Artemisia Annua

Alesaeidi¹,

Miraj²

2016

Electron physician

View full text Add to dashboard Cite

show abstract

“…MCF-7 cells were cultured in 25 t-flask and were maintained in Dulbecco's modified Eagle's medium supplemented with 100 IU/ mL penicillin, 100 μg/mL streptomycin, 10% fetal bovine serum at 37°°C with 5% CO 2 , 95% air and complete humidity [28].…”

Section: Mtt-assay Proceduresmentioning

confidence: 99%

Phytochemical and toxicity evaluation of Phaleria macrocarpa (Scheff.) Boerl by MCF-7 cell line and brine shrimp lethality bioassay

Azad¹

2016

J Coast Life Med

View full text Add to dashboard Cite

“…Also, pretreatment with sesamol enhances the irradiation efficacy in HeLa cells (11). Artemisia species are popular and traditional herbal medicines for the treatment of a variety of diseases and there have been a number of studies on their biological activities (12). Recent studies have shown the anticancer effect of some species of Artemisia, such as A. ciniformis (12) and A. absinthium (13).…”

Section: Introductionmentioning

confidence: 99%

“…Artemisia species are popular and traditional herbal medicines for the treatment of a variety of diseases and there have been a number of studies on their biological activities (12). Recent studies have shown the anticancer effect of some species of Artemisia, such as A. ciniformis (12) and A. absinthium (13). Another study has reported the cytotoxic effect of A. indica in four cancer cell lines, namely A-549, Caco-2, THP-1, and HEP-2 (14).…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic effects and radiosensitizing potential of Artemisia kopetdaghensis extract in human cervical cancel HeLa cells

Fanipakdel¹,

Hosseini²,

Afshar³

et al. 2019

Electron. physician

View full text Add to dashboard Cite

Background: Cervical cancer is one of the most common causes of cancer death in women worldwide; Radiotherapy has a major role in cervical cancer treatment. Anti-cancer effects of other species of Artemisia have been shown in some human cancer cells. Objective: To determine the cytotoxic and radiosensitizing effects of Artemisia kopetdaghensis extract on cervical cancer HeLa cells. Methods: Different concentrations of Artemisia kopetdaghensis extract (ART) (25-250µg/ml) were examined on HeLa cell line. Cell cytotoxicity of the extract and combination of extract plus 2Gy radiation was evaluated after 24, 48 and 72 hours via MTT assay. ART induced apoptosis was estimated with flow cytometry after 24h. Oneway analysis of variance (ANOVA) and then Bonferroni post hoc test were applied for statistical analysis. Prism (v.6) was used for all statistical analyses. Results: Artemisia kopetdaghensis decreased HeLa cells viability according to its concentration and timing of treatment. Comparing with the control group, a sub-G1 peak in the flow cytometry histogram of A. kopetdaghensis treated cells was shown, demonstrating that apoptosis was involved in A. kopetdaghensis cytotoxicity. Also, A. kopetdaghensis extract combined with irradiation, induced an additive cytotoxic effect on HeLa cells. Conclusion: Artemisia kopetdaghensis extract might be considered as a radiosensitizer in cervical cancer treatment potentially, and can be a good candidate for future studies. Cells were irradiated with dose rate of 120 centigray (cGy)/min from a 60Co γ-irradiation source (Theratone780, Canada). Source to surface distance (SSD) was 80 cm. Radiation dose was 2 Gray (Gy) radiated with 9×5 field size. Preparation of extractA. kopetdaghensis shrubs were collected from Gonabad (Northeast Iran) and identified by the herbarium of Ferdowsi University of Mashhad, Iran (voucher specimen number: 35205). The herb was dried and the extraction carried out for 48 h with 70% ethanol using a Soxhlet apparatus. After drying the extract in water bath, it was dissolved in DMSO and was stored at -20 °c. Cell cultureThe cells were cultured in DMEM which contained FBS (10%) and penicillin/streptomycin (100 unit/mL) then kept in an incubator (O2: 90%, CO2: 95%, temperature: 37 °C). Determination of A. kopetdaghensis extract cytotoxicity by MTT assayThe cells were exposed to various doses of extract (25-250 µg/ml) for 24, 48 and 72 h. After the passing of these times, for assessing cell metabolic activity, MTT solution (5 mg/mL) was added to each well. The plates were

show abstract

Cytotoxic and Apoptotic Effects of Extracts of Artemisia ciniformis Krasch. & Popov ex Poljakov on K562 and HL-60 Cell Lines

Cited by 20 publications

References 28 publications

A Systematic Review of Anti-malarial Properties, Immunosuppressive Properties, Anti-inflammatory Properties, and Anti-cancer Properties of Artemisia Annua

A Systematic Review of Anti-malarial Properties, Immunosuppressive Properties, Anti-inflammatory Properties, and Anti-cancer Properties of Artemisia Annua

Phytochemical and toxicity evaluation of Phaleria macrocarpa (Scheff.) Boerl by MCF-7 cell line and brine shrimp lethality bioassay

Cytotoxic effects and radiosensitizing potential of Artemisia kopetdaghensis extract in human cervical cancel HeLa cells

Contact Info

Product

Resources

About