Cytotoxic and apoptotic activity of Cheilanthes farinosa (Forsk.) Kaulf. against human hepatoma, Hep3B cells

Radhika, N Krishna; Sreejith, P. S.; Asha, V.V.

doi:10.1016/j.jep.2010.01.002

Cited by 28 publications

(16 citation statements)

References 14 publications

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“…Reports of therapeutic effectiveness, as well as scientific curiosity and the need for new drugs have prompted several groups to conduct pharmacological research on ferns and related plants. Pharmacological and ethnopharmacological studies have revealed that substances in ferns exhibit diverse pharmacological effects such as cytotoxicity (Radhika et al 2010), hepatoprotective activity (Wills and Asha 2006), antihyperglycemic activity (Zheng et al 2011a, b), leishmanicidal activity (Socolsky et al 2015), trypanocidal activity (Morais-Braga et al 2013a, b), anti-nociceptive activity, anti-inflammatory activity (Yonathan et al 2006), immunomodulatory activity (Wu et al 2005), and chemopreventive effects (Wills and Asha 2009). Because of the need for new medicines with such activities, pteridophytes and their secondary metabolites could potentially be of great medicinal value.…”

Section: Overview Of Fern Species Used In Medicinal Applicationsmentioning

confidence: 99%

Phytochemicals from fern species: potential for medicine applications

et al. 2017

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Ferns are an important phytogenetic bridge between lower and higher plants. Historically they have been used in many ways by humans, including as ornamental plants, domestic utensils, foods, and in handicrafts. In addition, they have found uses as medicinal herbs. Ferns produce a wide array of secondary metabolites endowed with different bioactivities that could potentially be useful in the treatment of many diseases. However, there is currently relatively little information in the literature on the phytochemicals present in ferns and their pharmacological applications, and the most recent review of the literature on the occurrence, chemotaxonomy and physiological activity of fern secondary metabolites was published over 20 years ago, by Soeder (Bot Rev 51:442–536, 1985). Here, we provide an updated review of this field, covering recent findings concerning the bioactive phytochemicals and pharmacology of fern species

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Section: Overview Of Fern Species Used In Medicinal Applicationsmentioning

confidence: 99%

Phytochemicals from fern species: potential for medicine applications

et al. 2017

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“…It has been reported that Cheilanthes farinosa (Forsk) Kaulf, a fern which is used to treat skin disorders also possessed strong anti-inflammatory and anti-nociceptive properties [5]. Radhika NK et al found the plant C. farinosa to produce considerable cytotoxic in hepatoma cell line, Hep 3B without inducing substantial damage to non-cancerous cell line RAW 264.7 [6]. Similarly different biological activities have been reported from the fern.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of in vitro and in vivo Biological Activities of Cheilanthes albomarginata Clarke

Lamichhane

Kim

Poudel

et al. 2014

BMC Complement Altern Med

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BackgroundThe Cheilanthes albomarginata Clarke (CA), a fern belonging to Pteridaceae family, is found mainly in India, Nepal, Pakistan and Bhutan at an altitude of 1300–2700 m. It grows mostly in the rock crevices on slopes. Juice from the rhizome of CA has been used to treat peptic ulcer. In this study, the biological activities (antioxidant, anti-inflammatory, anti-adipogenic and anti-obesity) of the extracts of CA were investigated. The total phenolic content of each extract was quantified. This is the first report regarding the study of biological activities on CA.MethodsIn the current study, the crude methanol and fractionated extract of the aerial part of CA were investigated for the antioxidant tests which were namely DPPH assay, hydrogen peroxide scavenging assay and nitrite scavenging assay. Their phenolic contents were measured by the Folin-Ciocalteu’s method.In vitro anti-inflammatory and anti-adipogenic assays were evaluated against the RAW 264.7 macrophage cells and 3 T3-L1 cells respectively. The crude methanol extract and phenolic fraction (combination of ethyl acetate and butanol fraction) were studied for the in vivo anti-obesity test using male Sprague Dawley rats.ResultsThe ethyl acetate fraction showed the strongest DPPH radical scavenging (82.54 ± 0.48%), hydrogen peroxide scavenging (3.41 ± 0.21 mg/ml) and nitrite scavenging activity (61.39%). The highest phenolic content was found in the ethyl acetate fraction followed by the butanol fraction. The ethyl acetate fraction showed the highest in vitro anti-inflammatory and anti-adipogenic activities. From the in vivo study on rats, the crude methanol extract and phenolic fraction showed plasma triglyceride lowering activity as well as reduction of weight of adipose tissue in high fat diet induced obese rats.ConclusionThe current study suggests that the ethyl acetate and butanol extracts of CA are potential source for antioxidant, anti-inflammatory and anti-adipogenic remedies. In addition to that the results of in vivo studies evidenced the possibility of CA as a source of anti-obesity drug remedies.

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“…The cells were seeded in 6 well plates and treated with different concentrations of GP extract (25, 50 and 100 g/mL), silymarin (50 g/mL), vehicle control (DMSO, 0.1%) for 24, 48 and 72 h. After treatment, the DNA fragmentation was assessed by method as described earlier (Radhika et al, 2010). Briefly, after extract treatment the cells were harvested, pelletized and treated with lysis buffer containing 1 M Tris, 5 M NaCl, 0.2 M EDTA and distilled water.…”

Section: Dna Fragmentation Analysismentioning

confidence: 99%