Cytotoxic and Apoptogenic Potential of Red Microalgal Polysaccharides

Gardeva, Elena; Toshkova, Reneta; Yossifova, L.; Minkova, Kaledona; Gigova, L.

doi:10.5504/bbeq.2012.0035

Cited by 15 publications

(8 citation statements)

References 36 publications

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“…EPSs all showed clear immunomodulation effects and inhibited the implanted S180 tumor’s growth in all doses used. Gardavea et al [ 65 ] studied the cytotoxic properties of polysaccharides from Dixoniella grisea and Porphyridium cruentum on two permanent human cell lines HeLa (cervical carcinoma) and MCF-7 (breast adenocarcinoma), as well as on primary culture from Graffi myeloid tumor in hamsters. Their investigations indicated that both algal polysaccharides showed high cytotoxic and apoptogenic activities on cancer cells and may be a promising alternative to synthetic substances.…”

Section: Discussionmentioning

confidence: 99%

Immunomodulatory, Antioxidant Activity and Cytotoxic Effect of Sulfated Polysaccharides from Porphyridium cruentum. (S.F.Gray) Nägeli

et al. 2021

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Porphyridium cruentum is a unicellular microalga that can synthesize and secrete to the culture medium-high amounts of polysaccharides. In this study, the immunomodulatory, cytotoxic effect and antioxidant activity of the sulfated polysaccharides (PcSPs) were determinate. The PcSPs were precipitated with 2% Cetylpyridinium bromide hydrate and ethanol and purified by dialysis. The extract was lyophilized for its characterization by Fourier transform-Infrared (FT-IR) spectroscopy and gas chromatography–mass spectrometry (GC-MS). The antioxidant activity of PcSPs were examined with assay 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) and compared with that of the biomass, observing significant differences between the results obtained from the PcSPs and biomass. To determine their ability to induce cytokine production Tumor Necrosis Factor alpha (TNF-α) and interleukina-6 (IL-6), the immunomodulatory activity of the PcSPs has been evaluated. In the mouse macrophage cell line (RAW 264.7), PcSPs are potent inducers of IL-6 cytokines but mainly of TNF-α. The cytotoxic capacity of PcSPs was measured by the MTT colorimetric assay in colorectal carcinoma (HTC-116), human leukemia (U-937 and HL-60), breast cancer (MCF-7), lung cancer (NCI-H460) and human gingival fibroblasts (HGF-1) cell lines. The IC50 value of 2311.20 µg mL−1, 1676.74 µg mL−1, 1089.63 µg mL−1, 5498.14 µg mL−1 and 2861.49 µg mL−1 respectively in the tumor lines and 5022.55 µg mL−1 in gingival fibroblasts were obtained. Our study suggested that PcSPs from P. cruentum have a moderate immunomodulatory and cytotoxic effect. The results obtained indicate that the polysaccharides from P. cruentum are potent inducers of IL-6 cytokines and, most importantly, of TNF-α. PcSPs showed no evidence of antigenic activity or hypersensitivity when administered intraperitoneally in mice. Furthermore, the in vivo study revealed an improvement of local inflammatory response against stress in the peritoneum. These findings suggest that the PcSPs from P. cruentum might have potential as a valuable ingredient in nutraceutical products.

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Section: Discussionmentioning

confidence: 99%

Immunomodulatory, Antioxidant Activity and Cytotoxic Effect of Sulfated Polysaccharides from Porphyridium cruentum. (S.F.Gray) Nägeli

et al. 2021

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“…Particular attention should be used, then, to prevent oxidation phenomena that would not guarantee the purity of the final product [65]. During the production of docosahexaenoic acid this algae also produces polysaccharides, of which are known antioxidant, anti-inflammatory, antiadhesive, anti-coagulant, anti-cancer, anti-viral and immunomodulating properties [66][67][68]. Numerous studies on various cancer cell lines show that marine polysaccharides have high cytotoxicity and apoptogenic activities that can be considered a future alternative for the production of natural antitumor drugs compared to synthetic drugs.…”

Section: No Deaths Have Been Recorded In Association Withmentioning

confidence: 99%

Dinoflagellates Important Marine Producers of Natural Bio-Compounds with High Biotechnological and Pharmacological Potential

Ferrara¹

2020

J Food Chem Nanotechnol

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“…The highly sulfated polysaccharides produced by the red microalgae Porphyridium cruentum and/ or Rhodella reticulata are known to be active against pathogenic bacteria and the fungus Candida albicans [13], retroviruses [36], MCF-7 and HeLa human cell lines [37], and diabetes in mice [38] (Table 1). Their anti-inflammatory [39] and antioxidant potential [40] is also known.…”

Section: Cyanophytamentioning

confidence: 99%

Biological activity of microalgae can be enhanced by manipulating the cultivation temperature and irradiance

Gacheva

Gigova

2014

Open Life Sciences

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The escalating levels of antibiotic resistance among pathogenic bacteria and the side effects of chemotherapeutic drugs in use forced the efforts of scientists to search for natural antimicrobial and anticancer substances with novel structures and unique mechanism of action. Focusing on bioproducts, recent trends in drug research have shown that microalgae (including the cyanobacteria) are promising organisms to furnish novel and safer biologically active compounds. Many microalgal metabolites have been found to possess potent antibacterial, antifungal, antiviral, anticancer and antiinflammatory activities, as well as antioxidant, enzyme inhibiting and immunostimulating properties. In this paper, the studies on the biological activity of microalgae associated with potential medical and pharmaceutical applications are briefly presented. Attention is focused on the impact of cultivation temperature, irradiance and growth stage on the biomass accumulation, activity and pathways of cell metabolism and the possibilities of using these variable factors to increase the diversity and quantity of biologically active substances synthesized by microalgae.

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Cytotoxic and Apoptogenic Potential of Red Microalgal Polysaccharides

Cited by 15 publications

References 36 publications

Immunomodulatory, Antioxidant Activity and Cytotoxic Effect of Sulfated Polysaccharides from Porphyridium cruentum. (S.F.Gray) Nägeli

Immunomodulatory, Antioxidant Activity and Cytotoxic Effect of Sulfated Polysaccharides from Porphyridium cruentum. (S.F.Gray) Nägeli

Dinoflagellates Important Marine Producers of Natural Bio-Compounds with High Biotechnological and Pharmacological Potential

Biological activity of microalgae can be enhanced by manipulating the cultivation temperature and irradiance

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