Cytotoxic and Antiproliferative Effects of Diarylheptanoids Isolated from Curcuma comosa Rhizomes on Leukaemic Cells

Viriyaadhammaa, Natsima; Saiai, Aroonchai; Neimkhum, Waranya; Nirachonkul, Wariya; Chaiyana, Wantida; Chiampanichayakul, Sawitree; Tima, Singkome; Usuki, Toyonobu; Duangmano, Suwit; Anuchapreeda, Songyot

doi:10.3390/molecules25225476

Cited by 12 publications

(26 citation statements)

References 22 publications

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“…In Thailand, ginger family plants are widely used as traditional medicine for many treatments, such as to relieve stomachache and hemorrhoids, as an herbal compress for massage, to improve blood circulation, to relieve muscular pain, and as a honey balm. Moreover, the rhizomes of these plants are also used as spices and ingredients for cooking [ 2 ]. Essential oils from Zingiberaceous plants have been used for mosquito control [ 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

Anticancer activity of Zingiber ottensii essential oil and its nanoformulations

et al. 2022

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Zingiber ottensii, is widely used in Asian traditional remedies for the treatment of many diseases. The present study explores anticancer activity of Z. ottensii essential oil (ZOEO) and its nanoformulations. ZOEO obtained from hydrodistillation of Z. ottensii fresh rhizomes was analysis using gas chromatography mass spectroscopy. Zerumbone (25.21%) was the major compound of ZOEO followed by sabinene (23.35%) and terpene-4-ol (15.97%). Four types of ZOEO loaded nanoformulations; nanoemulsion, microemulsion, nanoemulgels, and microemulgel, were developed. The average droplet size of the nanoemulsion and microemulsion was significantly smaller than that of the nanoemulgel and microemulgel. Comparison with other essential oils of plants of the same family on anticancer activity against A549, MCF-7, HeLa, and K562, ZOEO showed the highest cytotoxicity with IC50 of 43.37±6.69, 9.77±1.61, 23.25±7.73, and 60.49±9.41 μg/mL, respectively. Investigation using flow cytometry showed that ZOEO significantly increased the sub-G1 populations (cell death) in cell cycle analysis and induced cell apoptosis by apoptotic analysis. The developed nanoformulations significantly enhanced cytotoxicity of ZOEO, particularly against MCF-7 with the IC50 of 3.08±2.58, 0.74±0.45, 2.31±0.91, and 6.45±5.84 μg/mL, respectively. Among the four nanoformulations developed in the present study, nanoemulsion and microemulsion were superior to nanoemulgel and microemulgel in delivering ZOEO into cancer cells.

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Section: Introductionmentioning

confidence: 99%

Anticancer activity of Zingiber ottensii essential oil and its nanoformulations

et al. 2022

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“…This concentration was previously shown not to be toxic to peripheral blood mononuclear cells (PBMCs) [ 21 ]. In a previous report, DHH, referred to as compound 2 , did not induce hemolysis in red blood cells [ 17 ]. Thus, this concentration is presumed safe for normal human cells.…”

Section: Discussionmentioning

confidence: 99%

“…DHH was purified as previously described [ 17 ]. Briefly, rhizomes of Curcuma comosa were peeled and ground into a powder.…”

Section: Methodsmentioning

confidence: 99%

“…DHH has been purified by our research group from a hexane extraction protocol. At high concentrations DHH showed cytotoxic effects in the K562, KG1a, HL60, A549, and MCF-7 cell lines [ 17 ]. In our current work the ability of DHH to induce drug accumulation and decrease P-gp function in the drug-resistant leukemic cell line (K562/ADR) was examined.…”

Section: Introductionmentioning

confidence: 99%

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A Novel Drug Modulator Diarylheptanoid (trans-1,7-Diphenyl-5-hydroxy-1-heptene) from Curcuma comosa Rhizomes for P-glycoprotein Function and Apoptosis Induction in K652/ADR Leukemic Cells

Viriyaadhammaa

Duangmano

Saiai

et al. 2022

IJMS

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Curcuma comosa has been used in traditional Thai medicine to treat menstrual cycle-related symptoms in women. This study aims to evaluate the diarylheptanoid drug modulator, trans-1,7-diphenyl-5-hydroxy-1-heptene (DHH), in drug-resistant K562/ADR human leukemic cells. This compound was studied due to its effects on cell cytotoxicity, multidrug resistance (MDR) phenotype, P-glycoprotein (P-gp) expression, and P-gp function. We show that DHH itself is cytotoxic towards K562/ADR cells. However, DHH did not impact P-gp expression. The impact of DHH on the MDR phenotype in the K562/ADR cells was determined by co-treatment of cells with doxorubicin (Dox) and DHH using an MTT assay. The results showed that the DHH changed the MDR phenotype in the K562/ADR cells by decreasing the IC50 of Dox from 51.6 to 18.2 µM. Treating the cells with a nontoxic dose of DHH increased their sensitivity to Dox in P-gp expressing drug-resistant cells. The kinetics of P-gp mediated efflux of pirarubicin (THP) was used to monitor the P-gp function. DHH was shown to suppress THP efflux and resulted in enhanced apoptosis in the K562/ADR cells. These results demonstrate that DHH is a novel drug modulator of P-gp function and induces drug accumulation in the Dox-resistant K562 leukemic cell line.

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“…The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was used to determine the cytotoxicity of Kae-Lae extracts and active compounds on leukaemic cells. The method was described previously [ 40 ]. K562 (1.0 × 10 5 cells/mL), EoL-1 (3.0 × 10 5 cells/mL), and KG-1a (2.0 × 10 5 cells/mL) were added samples and then incubated for 48 h. The percentage of surviving cells was calculated using the following equation:…”

Section: Methodsmentioning

confidence: 99%

Biological activities of extracts and compounds from Thai Kae-Lae (Maclura cochinchinensis (Lour.) Corner)

Rueankham

Panyajai

Saiai

et al. 2023

BMC Complement Med Ther

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Background and aims The purpose of this study was to investigate the biological properties of Kae-Lae (Maclura cochinchinensis (Lour.) Corner), a traditional medicinal plant used in Ayurvedic recipes in Thailand. To achieve this objective, heartwood samples were collected from 12 sources across Thailand. Fractional extracts (n-hexane, ethyl acetate, and ethanol) and the dominant compounds (morin, resveratrol, and quercetin) were examined for their abilities on cytotoxicity, antioxidant, anti-inflammation, and antileukaemic activity (Wilms’ tumour 1 protein was used as a well-known biomarker for leukaemic cell proliferation). Methods The study used MTT to assess cytotoxicity in leukaemic cells (K562, EoL-1, and KG-1a). Antioxidant activities were evaluated using ABTS, DPPH, and FRAP assays. The anti-inflammatory activity was investigated by detecting IL-2, TNF-α, and NO using appropriate detection kits. Wilms’ tumour 1 protein expression was measured by Western blotting to determine the anti-leukaemic activity. The inhibition of cell migration was also analyzed to confirm anticancer progression. Results Among the tested extract fraction, ethyl acetate No. 001 displayed strong cytotoxicity specifically in EoL-1 cells, while n-hexane No. 008 demonstrated this effect in three cell lines. Resveratrol, on the other hand, displayed cytotoxicity in all the tested cells. Additionally, the three major compounds, morin, resveratrol, and quercetin, exhibited significant antioxidant and anti-inflammatory properties. In particular, resveratrol demonstrated a noteworthy decreased Wilms’ tumour 1 protein expression and a reduction in cell proliferation across all cells. Moreover, ethyl acetate No. 001, morin, and resveratrol effectively inhibited MCF-7 cell migration. None of these compounds showed any impact on red blood cell haemolysis. Conclusion Based on these findings, it can be concluded that Kae-Lae has promising chemotherapeutic potential against leukaemic cells, with fractional extracts (ethyl acetate and n-hexane) and resveratrol exhibiting the most potent cytotoxic, antioxidant, anti-inflammatory, and anti-cell migration activities.

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Cytotoxic and Antiproliferative Effects of Diarylheptanoids Isolated from Curcuma comosa Rhizomes on Leukaemic Cells

Cited by 12 publications

References 22 publications

Anticancer activity of Zingiber ottensii essential oil and its nanoformulations

Anticancer activity of Zingiber ottensii essential oil and its nanoformulations

A Novel Drug Modulator Diarylheptanoid (trans-1,7-Diphenyl-5-hydroxy-1-heptene) from Curcuma comosa Rhizomes for P-glycoprotein Function and Apoptosis Induction in K652/ADR Leukemic Cells

Biological activities of extracts and compounds from Thai Kae-Lae (Maclura cochinchinensis (Lour.) Corner)

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