Anticancer activity of Zingiber ottensii essential oil and its nanoformulations

Abstract

Zingiber ottensii, is widely used in Asian traditional remedies for the treatment of many diseases. The present study explores anticancer activity of Z. ottensii essential oil (ZOEO) and its nanoformulations. ZOEO obtained from hydrodistillation of Z. ottensii fresh rhizomes was analysis using gas chromatography mass spectroscopy. Zerumbone (25.21%) was the major compound of ZOEO followed by sabinene (23.35%) and terpene-4-ol (15.97%). Four types of ZOEO loaded nanoformulations; nanoemulsion, microemulsion, nanoemulgels, and microemulgel, were developed. The average droplet size of the nanoemulsion and microemulsion was significantly smaller than that of the nanoemulgel and microemulgel. Comparison with other essential oils of plants of the same family on anticancer activity against A549, MCF-7, HeLa, and K562, ZOEO showed the highest cytotoxicity with IC50 of 43.37±6.69, 9.77±1.61, 23.25±7.73, and 60.49±9.41 μg/mL, respectively. Investigation using flow cytometry showed that ZOEO significantly increased the sub-G1 populations (cell death) in cell cycle analysis and induced cell apoptosis by apoptotic analysis. The developed nanoformulations significantly enhanced cytotoxicity of ZOEO, particularly against MCF-7 with the IC50 of 3.08±2.58, 0.74±0.45, 2.31±0.91, and 6.45±5.84 μg/mL, respectively. Among the four nanoformulations developed in the present study, nanoemulsion and microemulsion were superior to nanoemulgel and microemulgel in delivering ZOEO into cancer cells.

Phytochemical Constituents and Antiproliferative Activities of Essential Oils from Four Varieties of Malaysian Zingiber officinale Roscoe against Human Cervical Cancer Cell Line

“…All essential oil samples displayed significant cell proliferation activity, in which the growth of the HeLa cells declined in a dose-dependent manner ( Figure 3 ). This result is in agreement with a study reported by Panyajai et al, where the oil of Zingiber ottensii also showed dose-dependent anticancer activity against the HeLa cell line [ 44 ]. The highest antiproliferative activity was shown by BA oil (IC 50 = 23.8 μg/mL), followed by BE (IC 50 = 35.3 μg/mL), SA (IC 50 = 41.3 μg/mL), and CH (IC 50 = 42.5 μg/mL).…”

Oxaliplatin-loaded nanoemulsion containingTeucrium poliumL. essential oil induces apoptosis in Colon cancer cell lines through ROS-mediated pathway

“…The marked elevations in ROS levels in cells treated with Oxa + TPO-NANO and TPO-NANO, relative to cells treated with Oxa-NS might be explained by the apoptotic potential of these two formulations on the studied cancer cells. Previous studies have demonstrated the anticancer potential of essential oils formulated into nanocarriers which predominantly triggered ROS production within cancer cells, thereby facilitating apoptosis of the treated cells (Al-otaibi, 2021 ; AlMotwaa, 2021 ; Salehi et al., 2020 ; Panyajai et al., 2022 ; Nirmala et al., 2020 ). Furthermore, TPO and its bioactive components have been shown to exert anticancer potential predominantly by induction of ROS generation and induction of cell death through the mitochondria-mediated apoptotic pathway (Su et al., 2013 ; Bai & Tang, 2020 ; Li et al., 2013 ; Sobral et al., 2014 ; Pan et al., 2016 ; Karan et al., 2018 ).…”

Nanomedicine as an Emerging Technology to Foster Application of Essential Oils to Fight Cancer

“…They obtained the greatest apoptotic action against MCF-7 breast cancer cells with the drug incorporated into lemon or salvia EO-based nanoemulsion, suggesting an enhanced solubilizing of the drug with this formulation. Recently, Panyajai et al [ 181 ] developed nanoformulations that significantly enhance the anticancer effects, especially against MCF-7, and show a superior internalization into cancer cells.…”