Cytotoxic activities of Coriolus versicolor (Yunzhi) extract on human leukemia and lymphoma cells by induction of apoptosis

Antiproliferative Activity of Kenyan Trametes versicolor Aqueous Extract on Selected Cancer and Normal Cell Lines

“…Such antiproliferative activity of the T. versicolor extract was reflected by its high percentage inhibition (Table 2) and the relatively low IC 50 values which were significantly not different to those of Tamoxifen, the reference drug used (Table 3). These findings are consistent with previous studies where ethanol-water extract of T. versicolor was reported to have significant inhibitory effects on human breast cancer cells, prostate cancer, human leukemia, and lymphoma cells [18]. In another study done by Cheong-Yip et al [36], ethanolwater extract of T. versicolor exhibited significant antiproliferative activity on three human breast cancer cell lines (MDA-MB-231, MCF-7 and T-47D) in a dose-dependent manner in vitro.…”

“…This was reflected by the low IC 50 values of 71.16 µg/ ml and 188.53 µg/ml on prostate cancer (DU145) and breast cancer cells (4T1) respectively compared to liver cancer cells (HCC), rat normal intestinal epithelial cells (IEC-6) and African green monkey normal kidney cells (Vero) both of which had an IC 50 value>1000 µg/ml (Table 3). These results tend to agree with previous reports that T. versicolor extracts can selectively suppress the proliferation of various cancer cells with no antiproliferative activity in normal cell lines suggesting its high level of safety [17,18,36]. In all cell lines used in this study, there was a significant difference in the proliferation rate between the untreated cells and the highest concentration (1000 µg/ml) of the T. versicolor extract used (Table 4).…”

“…The assay detects the reduction of MTT by mitochondrial dehydrogenase to blue formazan product, which reflects the normal function of mitochondria and cell viability [18]. Formazan, which is an insoluble yellow colored product can be measured spectrophotometrically [29,30] and the activity of mitochondrial dehydrogenase enzyme to produce formazan is directly and inversely proportional to the level of cell viability and inhibition respectively [31].…”

“…In some studies done in China to test for the antiproliferative activity of T. versicolor extract, results showed that the extract could inhibit the proliferation of four breast cancer cells (T-47D, Bcap37, ZR75-30, MCF-7), B-cell lymphoma (Raji), human promyelocytic leukemia (HL-60, NB-4) and liver cancer cell line (7703) [17,18]. However, the extracts of T. versicolor mushroom have been reported to be non-toxic to several normal cells [19][20][21] and in cancer patients on clinical trials [22][23][24].…”

Growth Inhibition and Induction of Apoptosis in Different Carcinoma Cell Lines by Euphorbia tirucalli Stem Extracts

“…This plate was incubated again for 4 h in CO2 incubator at 37°C. The resulting MTT-products were determined by measuring the absorbance at 570 nm using ELISA reader (Lau et al, 2004). Each point represents the mean of triplicate experiments.…”