Cytosolic targeting factor AKR2A captures chloroplast outer membrane-localized client proteins at the ribosome during translation

Kim, Dae Heon; Lee, Jae-Eun; Xu, Zheng‐Yi; Geem, Kyoung Rok; Kwon, Young–Kyu; Park, Chong-Yun; Hwang, Inhwan

doi:10.1038/ncomms7843

Cited by 31 publications

(20 citation statements)

References 71 publications

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“…Systematic functional analyses of rp paralog mutants need to account for such extra-ribosomal functions of RPs. For instance, uL23 recruits a nascent protein to its future localization in the chloroplast by coupling with its receptor ( Kim et al., 2015 ). Whether the differential uL23 paralog phenotypes are influenced by both moonlighting and ribosomal functions remains an open discussion.…”

Section: Functional Heterogeneity Of Rp Paralogsmentioning

confidence: 99%

Systematic Review of Plant Ribosome Heterogeneity and Specialization

et al. 2020

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Plants dedicate a high amount of energy and resources to the production of ribosomes. Historically, these multi-protein ribosome complexes have been considered static protein synthesis machines that are not subject to extensive regulation but only read mRNA and produce polypeptides accordingly. New and increasing evidence across various model organisms demonstrated the heterogeneous nature of ribosomes. This heterogeneity can constitute specialized ribosomes that regulate mRNA translation and control protein synthesis. A prominent example of ribosome heterogeneity is seen in the model plant, Arabidopsis thaliana , which, due to genome duplications, has multiple paralogs of each ribosomal protein (RP) gene. We support the notion of plant evolution directing high RP paralog divergence toward functional heterogeneity, underpinned in part by a vast resource of ribosome mutants that suggest specialization extends beyond the pleiotropic effects of single structural RPs or RP paralogs. Thus, Arabidopsis is a highly suitable model to study this phenomenon. Arabidopsis enables reverse genetics approaches that could provide evidence of ribosome specialization. In this review, we critically assess evidence of plant ribosome specialization and highlight steps along ribosome biogenesis in which heterogeneity may arise, filling the knowledge gaps in plant science by providing advanced insights from the human or yeast fields. We propose a data analysis pipeline that infers the heterogeneity of ribosome complexes and deviations from canonical structural compositions linked to stress events. This analysis pipeline can be extrapolated and enhanced by combination with other high-throughput methodologies, such as proteomics. Technologies, such as kinetic mass spectrometry and ribosome profiling, will be necessary to resolve the temporal and spatial aspects of translational regulation while the functional features of ribosomal subpopulations will become clear with the combination of reverse genetics and systems biology approaches.

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Section: Functional Heterogeneity Of Rp Paralogsmentioning

confidence: 99%

Systematic Review of Plant Ribosome Heterogeneity and Specialization

et al. 2020

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“…In addition to the TOC complex, potential AIMs were also identified in the outer envelope protein (OEP) complex, which serves as an alternative pathway for chloroplast protein import (Lee et al, 2014). OEP7 and OEP9 have been shown to function together with heat shock protein Hsp17.8 and AKR2A cofactors in targeting membrane proteins to plastid outer membranes under normal physiological conditions (Niehaus et al, 2014; Kim et al, 2015). To enable the efficient recognition by the autophagosomal or vacuolar membrane, it is possible that these chloroplast outer membrane proteins might serve as receptors by binding to the ATG or non-ATG chlorophagy regulators.…”

Section: Chlorophagy-related Membrane Receptors In Plantsmentioning

confidence: 99%

Chloroplast Degradation: Multiple Routes Into the Vacuole

Zhuang

Jiang

2019

Front. Plant Sci.

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Chloroplasts provide energy for all plants by producing sugar during photosynthesis. To adapt to various environmental and developmental cues, plants have developed specific strategies to control chloroplast homeostasis in plant cells, including chloroplast degradation during leaf senescence and the transition of chloroplasts into other types of plastids during the day-night cycle. In recent years, autophagy has emerged as an essential mechanism for selective degradation of chloroplast materials (also known as chlorophagy) in the vacuole. Different types of membrane structures have been implicated to involve in the delivery of distinct chloroplast contents. Here we provide a current overview on chlorophagy and discuss the possible chloroplast receptors and upstream signals in this process.

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“…Ankyrin-repeat containing protein 2 A (AKR2A) is an essential molecular chaperone that binds to hydrophobic amino acid residues, which prevents membrane proteins from aggregating after translation in the cytoplasm 6 – 8 . akr2a mutants display a chilling-sensitive phenotype, suggesting that AKR2A plays important roles in the plant network responding to cold stress.…”

Section: Introductionmentioning

confidence: 99%

The molecular chaperon AKR2A increases the mulberry chilling-tolerant capacity by maintaining SOD activity and unsaturated fatty acids composition

Chen

Hou

et al. 2018

Sci Rep

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Chilling is common in nature and can damage most plant species, particularly young leaves and buds. Mulberry (Morus spp.) is an economically important food source for the domesticated silkworm (Bombyx mori). However, weather and climatic extremes, such as “late spring coldness”, seriously damage mulberry buds and young leaves. The molecular mechanism involved in the differing mulberry chilling tolerance is unclear. In the present study, we found that mSOD1, mFADII, and mKCS1 interacted with mAKR2A and that the expression of mAKR2A, mSOD, mFAD, and mKCS1 in the chilling-tolerant mulberry variety was higher than that in the chilling-sensitive variety. Unsaturated fatty acids content and superoxide dismutase (SOD) activity in the chilling-tolerant variety was higher than that in the chilling-sensitive variety. After chilling treatment, mSOD1, mKCS1 and mAKR2A expression in the chilling-tolerant variety was reduced to lower than that in the chilling-sensitive variety, whereas mFADII expression increased in the chilling-tolerant variety compared with that in the chilling-sensitive variety, suggesting that the increased expression of the molecular chaperon mAKR2A helped to maintain or prompted the chilling-related proteins in the chilling-tolerant variety.

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Cytosolic targeting factor AKR2A captures chloroplast outer membrane-localized client proteins at the ribosome during translation

Cited by 31 publications

References 71 publications

Systematic Review of Plant Ribosome Heterogeneity and Specialization

Systematic Review of Plant Ribosome Heterogeneity and Specialization

Chloroplast Degradation: Multiple Routes Into the Vacuole

The molecular chaperon AKR2A increases the mulberry chilling-tolerant capacity by maintaining SOD activity and unsaturated fatty acids composition

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