The molecular chaperon AKR2A increases the mulberry chilling-tolerant capacity by maintaining SOD activity and unsaturated fatty acids composition

Abstract

Chilling is common in nature and can damage most plant species, particularly young leaves and buds. Mulberry (Morus spp.) is an economically important food source for the domesticated silkworm (Bombyx mori). However, weather and climatic extremes, such as “late spring coldness”, seriously damage mulberry buds and young leaves. The molecular mechanism involved in the differing mulberry chilling tolerance is unclear. In the present study, we found that mSOD1, mFADII, and mKCS1 interacted with mAKR2A and that the expression of mAKR2A, mSOD, mFAD, and mKCS1 in the chilling-tolerant mulberry variety was higher than that in the chilling-sensitive variety. Unsaturated fatty acids content and superoxide dismutase (SOD) activity in the chilling-tolerant variety was higher than that in the chilling-sensitive variety. After chilling treatment, mSOD1, mKCS1 and mAKR2A expression in the chilling-tolerant variety was reduced to lower than that in the chilling-sensitive variety, whereas mFADII expression increased in the chilling-tolerant variety compared with that in the chilling-sensitive variety, suggesting that the increased expression of the molecular chaperon mAKR2A helped to maintain or prompted the chilling-related proteins in the chilling-tolerant variety.

MaCDSP32 From Mulberry Enhances Resilience Post-drought by Regulating Antioxidant Activity and the Osmotic Content in Transgenic Tobacco

“…We conjecture that the intricate regulatory network enmeshing MaCDSP32 is responsible for the rapid wilting of the transgenic lines during severe water deficit and their maintenance of the minimum water consumption, which allows their survival under drought conditions. The SOD enzyme is the main site of the conversion of superfluous O 2 − via its disproportionation in the chloroplast (Chen et al, 2018). Based on the results obtained for SOD activity and O 2 − production demonstrate that MaCDSP32 plays a key role in the regulation of ROS homeostasis by particularly targeting the SOD enzyme.…”

“…This result indicated that MaCDSP32 might affect the activity of chlorophyll-related enzymes under NaCl stress. Although NaCl treatment can increase the activity of chlorophyllase, the synthesis and degradation of chlorophyll are mainly affected by the activity of chlorophyll enzymes (Chen et al, 2018). This increased chlorophyllase activity might induce CDSP32 activity, which functions to protect photosynthetic system elements (Broin et al, 2002).…”

“…The relative expression of genes was calculated based on the threshold cycle according to the Ct method (Schmittgen and Livak, 2008). The result was normalized to the expression of the reference gene actin in mulberry (Chen et al, 2018) or L25 in tobacco (Liu et al, 2018). Expression was determined for at least three replicates per treatment.…”

Comprehensive analysis of the gene expression profile of wheat at the crossroads of heat, drought and combined stress

“…Additionally, it is important for chloroplast biogenesis, and increases plant biological system capacity for abiotic stresses [61,62]. On the other hand, AAP6 is correlated with plant drought tolerance [63].…”

Selection of reference genes for RT‐qPCR normalization in blueberry (Vaccinium corymbosum × angustifolium) under various abiotic stresses

“…Abiotic stresses are nonbiological factors that influence living organisms in a specific environment that negatively affects the growth and yields of crop [3]. High salinity [2,4], drought [4,5], flooding [6,7], chilling [8][9][10], heat [4,10] and heavy metal stresses [11][12][13][14][15][16] are adverse natural factors that affect fruit tree growth and yield that are undesirable to be encountered during the whole process.…”