Cytoprotective Effects of Curcumin on Sodium Fluoride-Induced Intoxication in Rat Erythrocytes

Abstract

Curcumin is well known for its potent antioxidant activity. The result of numerous studies showed that antioxidants can protect against fluoride-induced toxicity. In the present study, protective effects of curcumin against sodium fluoride-induced toxicity in rat erythrocytes were evaluated. Curcumin (10 and 20 mg/kg) and vitamin C (10 mg/kg) were administrated intraperitoneally for 1 week followed by sodium fluoride (600 ppm) treatment for next week. Erythrocytes were isolated and superoxide dismutase and catalase activities as well as the levels of reduced glutathione, and lipid peroxidation were measured. The level of malondialdehyde in sodium fluoride treated rats (595.13 ± 20.23 nmol/g Hb) increased compared to the normal rats (315.44 ± 9.76 nmol/g Hb). Animals which were pretreated with curcumin at 20 mg/kg for 1 week prior to sodium fluoride intoxication showed significant reduction in the malondialdehyde level (320.22 ± 9.28 nmol/g Hb). Also, pretreated with curcumin (20 mg/kg) and vitamin C restored the superoxide dismutase and catalase activities and modified the level of reduced glutathione compared with control group (p > 0.01).

In Vivo Protective Effects of Gallic Acid Isolated from Peltiphyllum Peltatum Against Sodium Fluoride-Induced Oxidative Stress in Rat Erythrocytes

“…Furthermore, we found that glutathione levels in erythrocytes diminished after NaF exposure, suggesting an induction of oxidative stress. These fi ndings, together with the results obtained for the positive control, vitamin C, were in agreement with our previous study (20) where in vivo amelioration of NaF-mediated oxidative stress was recorded in curcumin pre-treated rats. Our fi nding that gallic acid effectively protected rat erythrocytes suggests that the antioxidant effect of the compound at the tested dosage in vivo was more prevalent than its prooxidative effects.…”

“…In a previous study (20), we introduced a rat model of fl uoride-induced oxidative damage. In order to continue our work on P. peltatum and validate the therapeutic potential of gallic acid from natural sources, the present study was designed to study the in vivo protective effect of gallic acid against oxidative damages in rat erythrocytes.…”

Ameliorative Effects of Quercetin on Sodium Fluoride-Induced Oxidative Stress in Rat’s Kidney

“…19 As an efficient reactive oxygen scavenger including NO and iron chelator, it appears that quercetin improves the oxidant-antioxidant imbalance created by fluoride intoxication. 21 As a multifunctional molecule, however, other mechanisms of action are also likely to be associated with quercetins nephroprotective effect.…”

Antioxidant, antihemolytic, and inhibitory activities of endemicPrimula heterochromaagainst Fe2+-induced lipid peroxidation and oxidative stress in rat brainin vitro

“…Natural product such as phenolics and flavonoids can protect the body against free radical induced toxicity through different antioxidative mechanisms (Nabavi et al, 2012b).…”