Cytoprotective effect of S-adenosylmethionine compared with that of misoprostol against ethanol-, aspirin-, and stress-induced gastric damage

Laudanno, Oscar

doi:10.1016/0002-9343(87)90850-3

Cited by 18 publications

(9 citation statements)

References 16 publications

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“…13 The use of SAM, even at concentrations much higher than the therapeutic doses, does not cause any damage to the gastrointestinal lining. 43,44 Therefore, concentrations used in the 3D experiments should not exert any toxic effect.…”

Section: Discussionmentioning

confidence: 99%

S‐adenosyl L‐methionine inhibits azoxymethane‐induced colonic aberrant crypt foci in F344 rats and suppresses human colon cancer Caco‐2 cell growth in 3D culture

Guruswamy

Swamy

Choi

et al. 2007

Intl Journal of Cancer

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S-Adenosyl L-methionine (SAM) is a universal methyl group donor to various intermediary metabolites, hormones, proteins, neurotransmitters, phospholipids and nucleic acids. Deficiency of folate, which plays a role in the synthesis of SAM leads to increased risk for colon cancer. This study tested the effectiveness of SAM supplementation in protecting against azoxymethane (AOM)-induced colon carcinogenesis in male F344 rats. We also tested the effect of SAM on cyclooxygenase-2 (COX-2) in a macrophage cell line. Further, we developed a 3-D culture model using Caco-2 cells to test the effect of SAM on tumor spheroid size and number. Groups of rats were given the experimental diet containing either 0-, 400-or 800-ppm SAM, 1 week before the first AOM injection and continued until 8 weeks. In the control group, AOM produced a substantial number of aberrant crypt foci (ACF) (96 6 8). Dietary administration of SAM significantly reduced the number of total ACF (400 ppm SAM, 68 6 7.3, p < 0.01 and 800 ppm SAM, 57 6 7.1, p < 0.001). SAM significantly decreased AOM-induced colonic multicrypt foci in a dose-dependent manner. Suppression of Lipopolysaccharide (LPS) induced COX-2 protein expression was observed in a RAW264.7 cell line. We established growth of Caco-2 cells as spheroids, in a 3D matrix of collagen and matrigel. Treatment with SAM decreased both size and number of spheroids in a dose-dependent manner (p < 0.0001). These observations demonstrate for the first time that SAM can reduce the occurrence of ACF in AOM treated male F344 rats and suppress formation of human tumor spheroids and expression of COX-2. ' 2007 Wiley-Liss, Inc.

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Section: Discussionmentioning

confidence: 99%

S‐adenosyl L‐methionine inhibits azoxymethane‐induced colonic aberrant crypt foci in F344 rats and suppresses human colon cancer Caco‐2 cell growth in 3D culture

Guruswamy

Swamy

Choi

et al. 2007

Intl Journal of Cancer

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“…Animal maintenance and experiments were in accordance with National Institutes of Health (NIH) guidelines and were approved by the Institutional Animal Care and Use Committee of the Rush University of Medical Center, Chicago, IL. Adoptively transferred EAE was induced in 4- to 5-week-old female SJL/J mice (Harlan Sprague Dawley, Indianapolis, IN), as described (16–18, 40, 41). Donor mice were immunized subcutaneously with 400 µg of bovine MBP and 60 µg of M. tuberculosis in IFA (16–18, 41).…”

Section: Methodsmentioning

confidence: 99%

“…Experimental animals were scored by a masked investigator, as follows: 0, no clinical disease; 0.5, piloerection; 1, tail weakness; 1.5, tail paralysis; 2, hindlimb weakness; 3, hindlimb paralysis; 3.5, forelimb weakness; 4, forelimb paralysis; 5, moribund or death. To induce chronic EAE, C57BL/6 mice were immunized with 100 µg of MOG35–55, as described (40). Mice also received two doses of pertussis toxin (150 ng per mouse) on 0 and 2 days post-immunization.…”

Section: Methodsmentioning

confidence: 99%

Aspirin ameliorates experimental autoimmune encephalomyelitis through interleukin-11–mediated protection of regulatory T cells

et al. 2018

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Multiple sclerosis (MS) is a human disease that results from autoimmune T cells targeting myelin protein that is expressed within the central nervous system. In MS, the number of FoxP3-expressing regulatory T cells (Tregs) is reduced, which facilitates the activation of autoreactive T cells. Because aspirin (acetylsalicylic acid) is the most widely used nonsteroidal anti-inflammatory drug, we examined its immunomodulatory effect in mice with experimental autoimmune encephalomyelitis (EAE), an animal model of MS. We found that low-dose aspirin suppressed the clinical symptoms of EAE in mouse models of both relapsing-remitting and chronic disease. Aspirin reduced the development of EAE driven by myelin basic protein (MBP)–specific T cells and the associated perivascular cuffing, inflammation, and demyelination. The effects of aspirin required the presence of CD25+FoxP3+ Tregs. Aspirin increased the amounts of Foxp3 and interleukin-4 (IL-4) in T cells and suppressed the differentiation of naïve T cells into T helper 17 (TH17) and TH1 cells. Aspirin also increased the transcription of Il11 mediated by the transcription factor CREB, which was necessary for the generation of Tregs. Neutralization of IL-11 negated the effects of aspirin on Treg development and exacerbated EAE. Furthermore, we found that IL-11 alone was sufficient to maintain the percentage of FoxP3+ Tregs and protect mice from EAE. These results identify a previously uncharacterized mode of action of aspirin.

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“…Using these primary MSCs, we confirmed that cell viability and endogenous IGF-I levels dosedependently increased with SAM treatment, with a significant increase in response to 10 µM SAM. S-Adenosyl-L-methionine (SAM) is a methyl donor that exerts anti-oxidative and cytoprotective effects under various conditions [14][15][16]. SAM is particularly important for opposing the toxicity of free radicals generated by various toxins [35].…”

Section: Discussionmentioning

confidence: 99%

“…SAM is particularly important for reducing free radical toxicity from various toxins [14]. The cytoprotective effect of SAM is well-known [15,16]. However, the cytoprotective effect of SAM on oxidative stress-induced apoptosis has not been studied in murine MSCs.…”

Section: Introductionmentioning

confidence: 99%

Protective Effect of S-Adenosyl-L-Methionine on Oxidative Stress-Induced Apoptosis Regulates Nrf-2 via IGF-I in Rat Bone Marrow Mesenchymal Stem Cells

Oh¹,

Kim²,

Kang³

2016

J Stem Cell Res Ther

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Bone marrow mesenchymal stem cell (MSC)-mediated regeneration is a promising treatment for degenerative diseases and traumatic injuries. S-Adenosyl-L-methionine (SAM) is the principal biological methyl donor. We hypothesized that the cytoprotective effect of SAM on oxidative stress-induced apoptosis in rat MSCs is due to insulin-like growth factor-I (IGF-I) and nuclear factor erythroid 2-related factor 2 (Nrf2). SAM (10 µM) increased both endogenous IGF-I levels and cell viability (p<0.05). In MSCs, 1 mM H 2 O 2 at 6 h significantly decreased cell viability and IGF-I and Nrf2 activation, but increased ROS generation (p<0.05 and p<0.01, respectively). The decrease in cell viability, endogenous IGF-1, and Nrf2 in H 2 O 2-induced apoptosis was recovered by SAM (10 µM). Apoptosis induced by H 2 O 2 also decreased Nrf2 activity, as determined by immunofluorescence staining, but the decrease was also recovered by SAM. We demonstrated that H 2 O 2-induced apoptosis was reduced by SAM through Annexin-V. Using a specific small interfering RNA (siRNA), the increase in SAM-induced cell viability and endogenous Nrf2 in the presence of H 2 O 2 was suppressed by IGF-I siRNA, but the endogenous IGF-I level was not changed by Nrf2 siRNA. The increase in endogenous Nrf2 with exogenous IGF-I and H 2 O 2 treatment was also suppressed by Nrf2 siRNA, but the IGF-I level was not inhibited (p<0.05). These results suggest that the cytoprotective effect of SAM against H 2 O 2induced apoptosis is mediated by increased Nrf2 activity through IGF-I. These factors could regulate the metabolic fate and survival of MSCs.

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Cytoprotective effect of S-adenosylmethionine compared with that of misoprostol against ethanol-, aspirin-, and stress-induced gastric damage

Cited by 18 publications

References 16 publications

S‐adenosyl L‐methionine inhibits azoxymethane‐induced colonic aberrant crypt foci in F344 rats and suppresses human colon cancer Caco‐2 cell growth in 3D culture

S‐adenosyl L‐methionine inhibits azoxymethane‐induced colonic aberrant crypt foci in F344 rats and suppresses human colon cancer Caco‐2 cell growth in 3D culture

Aspirin ameliorates experimental autoimmune encephalomyelitis through interleukin-11–mediated protection of regulatory T cells

Protective Effect of S-Adenosyl-L-Methionine on Oxidative Stress-Induced Apoptosis Regulates Nrf-2 via IGF-I in Rat Bone Marrow Mesenchymal Stem Cells

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