Cytoprotection of Antioxidant Biocompounds from Grape Pomace: Further Exfoliant Phytoactive Ingredients for Cosmetic Products

Abstract

The goal of this study was to investigate the efficacy and the safety of grape pomace (Vitis labrusca L.) obtained from the winemaking process as an antioxidant raw material for cosmetic formulations. Grape pomace was dried and submitted to extraction with solvents. The extracts were filtered and freeze-dried. The extraction carried out with 75% acetone-water showed a better yield (1.9 g·g−1). High performance liquid chromatography (HPLC) analysis confirmed the presence of ellagic acid as an active biocompound in the extracts. The Total phenolic content of freeze-dried extracts reached 69.83 ± 1.02 mg for 75% acetone-water system. Antioxidant activity determined by the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method revealed there was no statistical difference between the extract (EC50 6.9 ± 0.21) and butylated hydroxytoluene (BHT) (EC50 7.6 ± 0.71). Results of cytotoxicity showed that extracts from grape pomace were safe, even at the highest concentration tested (200 mg·mL−1) against the 3T3 cell line. Scanning Electron Microscopy (SEM) analysis revealed that there were no morphological cell changes of cells treated. Cytoprotection of cells towards the oxidation promoted by the peroxide solution was observed in the lowest concentration tested (0.73 mg·mL−1). Grape pomace from the winemaking process revealed a significant antioxidant activity attributed to its biocompounds. The safety and efficacy of extracts of grape pomace as raw material for cosmetic formulations was proposed through cell culture assays.

Potential health benefits of phenolic compounds in grape processing by-products

“…Additionally, fractionation enhanced the active polyphenols present in both crude extracts and intensified the inhibitory activity against collagenase, suggesting the suitability of the extracts for topical cosmetic formulations, which commonly contain between 25 and 100 lmol polyphenols/L (Maidin et al, 2018;Zillich et al, 2015). The potential of GP-derived polyphenols as an antioxidant material in cosmetic formulations was confirmed in another study in which GP extract diminished the cytotoxicity of hydrogen peroxide (H 2 O 2 ) in mouse fibroblasts (Maluf et al, 2018).…”

Research Advances in the Use of Bioactive Compounds from Vitis vinifera By-Products in Oral Care

“…Grape pomace is another V. vinifera by-product with important free radical scavenging activities, able to prevent lipid peroxidation [ 152 ]. In this context, a cytoprotective effect of grape pomace in H 2 O 2 induced oxidative stress was investigated by Maluf et al, demonstrating a beneficial impact even at the lowest concentration tested [ 153 ]. These results are in line with the findings of Goutzourelas et al, who demonstrated that, following the exposure to grape pomace extract, the enzymatic activity of gamma-glutamylcysteine synthetase and glutathione S-transferase from endothelial and muscle cells increased [ 129 ].…”

Antioxidant Activity and Phytochemical Screening of the Apricot Cake Extract: Experimental and Theoretical Studies

“…A theoretical calculation was conducted to investigate the antioxidant activity of the main compounds of apricot pomace extract. The molecules structure of the apricot pomace extract (1)(2)(3)(4)(5)(6)(7)(8) obtained after a geometric optimization procedure is presented in Fig. 4.…”

“…In this context, the cheap waste products are particularly interesting for the environmental and economic benefits resulting from their re-use. Agro-industrial by-products are cheap, abundant and sustainable resources which contain compounds with antioxidant, cytotoxic and antimicrobial activities that can be used for synthesis of nanoparticles [4,5], production of food additives [6], as a natural preservative and antioxidant in cosmetic products [7], for inhibition corrosion of metals in corrosive media 1 National Technical University of Ukraine "Igor Sikorsky Kyiv Polytechnic Institute", 37, Peremogy Ave., 03056 Kyiv, Ukraine 2 Ukrainian State Chemical-Engineering University, 8, Gagarina Ave., 49066 Dnipro, Ukraine * vorobyovavika1988@gmail.com Ó Vorobyova V., Shakun A., Chygyrynets' O., Skiba M., Zaporozhets J., 2020 [8][9][10][11]14]. Some studies have already been done on byproducts, which could be potential sources of antioxidants [1][2][3][4][5].…”