Cytoplasmic RNA from normal and malignant human cells shows homology to the DNAs of Epstein-Barr virus and human adenoviruses.

Abstract

Cytoplasmic RNA prepared from several human cell lines and tissues was hybridised to DNA from Epstein‐Barr virus, human adenovirus types 2, 3 and 12 and human papovaviruses BK and JC. RNA from all the cells, regardless of whether or not they were virally infected, hybridised to specific regions of the Epstein‐Barr virus or adenovirus genomes but not to papovavirus DNA. The cellular cross‐hybridising species appear to be repetitive sequences which are conserved in higher eukaryotes. Mismatch estimations indicate a high degree of homology between the viral and host sequences. Detailed analysis of selected regions of viral DNA failed to reveal any primary‐structural peculiarities.

A Comparative Study of the in vitro and in vivo Antiviral Activities of Acyclovir and Penciclovir

“…(Hinuma et al, 1967) were grown as described previously (Arrand et al, 1983;Collins and Ellis, 1993). HSV-1 strain SC16 and HSV-2 strain 186 have been described previously (Rawls et al, 1968;Hill et al, 1975).…”

Hypothesis: a novel route for immortalization of epithelial cells by Epstein-Barr virus

“…Step 1: Integration of circular p31 DNA (v) (stippled and solid bar) (Karran et al, 1990) into the linear host (h) chromosome (open bar, which also contains some sequence homology with EBV DNA (Arrand et al, 1983; represented by solid bar), assuming that viral information initially integrates into the host, possibly at or near a sequence homologous region;…”

“…PCR products were separated by electrophoresis on 1% agarose gels containing ethidium bromide. Following transfer to membranes, their identities were veri®ed by hybridization with EBV sub-fragments ( Figure 1 and Table 1) (Arrand et al, 1983), labelled with a-32 P-dCTP using the random prime labelling system, as recommended by the manufacturer (Pharmacia Biotech). (The large BamHI A fragment, was initially cleaved with BglII in order to generate probes of higher speci®city.)…”

Immunological studies on the Epstein--Barr virus encoded alkaline deoxyribonuclease found in virus-producing lymphoblastoid cells

“…The cell lines used included B95-8 (a marmoset B cell EBV producer line), Raji (a Burkitt's tumour non-producer B cell line), P3HR-1 (a Burkitt's tumour producer B cell line; the virus produced is non-transforming), Namalwa (a latently infected B cell line from a Burkitt's tumour) and DG-75 and Ball-1 cells (both EBV-negative human B cell lines) (Adams, 1979). Cells were maintained as described previously (Arrand et al, 1983). EBV antigen production was induced by treating cultures at a starting density of 3 x 105 cells/ml with TPA and 5-bromodeoxyuridine (BUdR) at a final concentration of 50ng/ml and 50 ~tg/ml respectively (Bauer, 1983).…”