Cytophotometric Determination of the Amount of Dna in Arachis L. Sect. Arachis (Leguminosae)

Abstract

The 2C amounts of DNA for 12 taxa of the section Arachis nom. nud. of the genus Arachis L. were determined using cytophotometric techniques. The diploid taxa ranged from 4.92 to 5.98 pg of DNA per cell. The species of the diploid series Annuae Krap. & Greg. nom. nud. averaged ca. 1 pg less DNA per cell than the taxa of the diploid series Perennes Krap. & Greg. nom. nud. No significant differences were found between taxa within these two series. The tetraploid taxa ranged from 10.36 to 11.35 pg of DNA per cell. Within the tetraploid series Amphiploides Krap. & Greg. nom. nud. differences were found between A. monticola Krap. & Rig. and A. hypogaea L. The two subspecies of A. hypogaea, ssp. hypogaea and ssp. fastigiata Waldron, were found to differ significantly in their 2C amounts of DNA. The implications of the cytophotometric data on the chromosomal evolution of this section are discussed.

Variation in 4C DNA content and chromosome characteristics in different varieties of Coriandrum sativum L.

“…Seeds were obtained from Sutton seed nursery, Calcutta and through the courtesy of Komarov Bo tanical Institute, USSR; Karl-Marx University, Leipzig and Chelsea Physic Garden, London. Seeds were grown in the experimental plot of the Department of Botany, University of Cal cutta. Das C. sativum L. However, such differences observed in the present work, are in accordance with the findings of other workers (Nishikawa 1970, Furuta et al 1975, Narayan and Rees 1976, Price et al 1980, Resslar et al 1981, Raina and Rees 1983, Lavania 1985, Mukherjee and Sharma 1986, Banerjee and Sharma 1987). The present investigation shows that the 4C DNA amount in a variety was constant and differed .…”

Nuclear DNA and RNA amounts in the wild and cultivated urd and mung beans and their M1 plants.

“…Nagato et al (1981) demonstrated that there is a reduction in the 2C DNA during the evolution of Oryza sativa from O. perennis. Resslar et al (1981) suggested that in the genus Arachis, the increase in DNA amount led to the evolution of subsp. fastigiata from the wild progenitor A. monticola, but the decrease in 2C DNA has led to the evolution of subsp.…”

“…Various reports so far indicate that chromosomal DNA amount is changeable to some extent within a species (Jones and Rees 1968, Ayonoadu 1974, Belford and Thompson 1981, Nagato et al 1981, Yamamoto and Nagato 1984. Although enough reports are there with regard to the DNA amounts in various crops (Nagato et al 1981, Resslar et al 1981) not much work has been done on the mutant lines. In the present investigation, we examined the variation of DNA and RNA amounts in Vigna sublobata (Roxb.)…”

Genome Size Variation in Central European Species of Cirsium (Compositae) and their Natural Hybrids

“…Authors who have studied the correlation between genome size and longevity (life cycle, life-history or minimum generation time) have repeatedly confirmed that annuals have smaller genomes when compared with their perennial relatives; e.g. Rees and Hazarika (1969) or Nandini et al (1997) in Lathyrus; Nagl and Ehrendorfer (1974) in the tribe Anthemideae; Price and Bachmann (1975) in Microseris; Jones and Brown (1976) in Crepis; Resslar et al (1981); Hiremath and Salimath (1991) in Eleusine; Srivastava and Lavania (1991) in Papaver, Naranjo et al (1998) in Vicia; Watanabe et al (1999) in. Brachyscome; Torrell and Vallès (2001) in Artemisia.…”