Cytomorphometry of canine cutaneous histiocytoma

Paździor-Czapula, Katarzyna; Otrocka-Domagała, Iwona; Rotkiewicz, T.; Gesek, M.

doi:10.2478/pjvs-2014-0059

Cited by 4 publications

(2 citation statements)

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“…Interestingly, spontaneous regression occurs after some time (1). Studies investigating this entity have noted a change in phenotype and cytomorphology during CCH regression and attributed the increased immune cell infiltration to a more mature phenotype of the tumor cells (2)(3)(4)(5). In addition, the ability of CCH derived tumor cells to stimulate lymphocytes was shown in vitro (6).…”

Section: Introductionmentioning

confidence: 99%

Immune checkpoint regulation is critically involved in canine cutaneous histiocytoma regression

Diehl,

Hansmann

2024

Front. Vet. Sci.

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IntroductionCanine cutaneous histiocytoma (CCH) is a benign tumor frequently occurring in young dogs which is derived from Langerhans cells (LC). Distinguishing features of this tumor are its spontaneous regression following a rapid tumor growth. Impaired control of immune checkpoints during tumor development and progression is a widespread phenomenon which may result in an absent or ineffective immune response. The interaction between the inflammatory response and the expression of immune checkpoint molecules is only partially described in this tumor type. The aim of this study was to identify immune checkpoint molecules and molecules from the interferon-mediated immune response that are involved in the regression of CCH.MethodsForty-eight CCH derived from dogs ≤ 4 years of age were assigned to one of four groups according to the severity and distribution of lymphocyte infiltration. Using immunohistochemistry and whole-slide image scans of consecutive sections the expression of programmed death protein ligand 1 (PD-L1), CD80, CD86, Survivin, forkhead box protein 3, Ki-67, cleaved caspase-3, CD3, and mx1 were investigated. RNA in-situ hybridization was performed for transcripts of mx1 and interferon-γ.ResultsNeoplastic cells showed an expression of PD-L1, CD80, CD86, and Survivin. The density of CD80 expressing cells was negatively correlated with regression while the density of cleaved caspase-3 positive cells increased with regression. Mx1 transcripts and protein were predominantly localized in neoplastic cells while interferon-γ transcripts were most frequently detected in T-cells.ConclusionThe expression of the immune checkpoint molecules CD86 and PD-L1 and particularly the reduced expression of CD80 in groups 3 and 4 indicate an influence of the investigated immune checkpoints on tumor regression. In parallel an activation of the apoptotic cascade during regression is suggested. Finally, the detection of mx1 within the neoplasm pinpoints to a yet undisclosed role of anti-cellular signaling in tumor immunity.

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Section: Introductionmentioning

confidence: 99%

Immune checkpoint regulation is critically involved in canine cutaneous histiocytoma regression

Diehl,

Hansmann

2024

Front. Vet. Sci.

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“…a result of neoplastic proliferation of epidermal dendritic cells, called the Langerhans cells. It is classified as a tumour of round cells, and is reported as the most frequent cutaneous neoplasm in dogs (1)(2)(3). CCH has similarities to some forms of human Langerhans cell histiocytosis (LCH), nevertheless, LCH has distinct clinical behaviour between dogs and humans (4).…”

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confidence: 99%

A Role for Angiogenesis in Canine Cutaneous Histiocytoma Regression: Insights into an Old Clinical Enigma

Costa

Ferreira

Prada

et al. 2020

In Vivo

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Background/Aim: Canine Cutaneous Histiocytoma (CCH) is a Langerhans' cells benign tumour that undergoes spontaneous regression. The aim of the present study was to investigate the role of angiogenesis, a key step for tumour development, in CCH regression. Materials and Methods: 50 CCH samples were classified into 4 histological groups according to a regression scale, and evaluated for expression of vascular endothelial factor-A (VEGF-A) and its receptor VEGFR-2 as well as microvessel density (MVD). Results: Tumours during early stages of the regressive process had a lower MVD compared to later stages, while CCH tumoural cells showed a limited production of VEGF, but higher levels of VEGFR-2. On the contrary, tumours in advanced phases of regression showed a higher number of neovessels, probably associated with the inflammatory state and the healing process. Conclusion: Our results suggest that angiogenesis may be compromised at early stages of histiocytoma development and this may be a determinant of regression in this tumour.Canine cutaneous histiocytoma (CCH) is a benign tumour on the dog's skin that, in almost all cases, undergoes spontaneous regression. It can occur anywhere on the dog's skin surface as 3279 This article is freely accessible online.

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