Cytomorphometric and immunohistochemical results of regenerate bone after intraosseous injection of the drug "perftoran" at the оsteosynthesis of femoral neck fractures (experimental study)

Abstract

The purpose of this study is to analyze the immune-histochemical results of cytomorphometric bone regenerate after intraosseous injection the "Perftoran" with experimental osteosynthesis of femoral neck frac-tures. Materials and methods. As experimental animals were male rabbits Chinchilla aged 6 months. In all the animals the femoral neck fracture was formed transcervically osteotomy site and the femoral head ligament was crossed. These cytomorphometric and immune-histochemical studies of regenerate bone fracture area injected were analyzed in the dynamics in 3 series of animals. The first (control) series (n=20) did not carry out a fracture fixation, and the head of the thigh was injected oxygenated drug the Perftoran. In the second series (n=24) a fracture was synthesized by bioactive 3-spokes, the drug Perftoran did not injected. In the third series (n=24) a fracture was fixed by means of 3 bioactive broken spokes and the oxygenated drug Perftoran was injected in head hip intraoperatively. Results and discussion. In the first series of experiments, the radiographic and macroscopic results did not revealed signs of fracture healing, it was observed a formation of a false joint. In the series with internal fixation of femoral neck fractures by bioactive spokes it was identified fracture healing. In the 2 serie to 60 days, it was noted an increase in the number density of osteoclasts and a gradual reduction in the surface area of trabeculae, indicating that the ongoing process of reparative regeneration with active restructuring of bone tissue. In the 3 serie with the Perftoran intraosseous injection, a cellular composition of bone regeneration was significantly cor-responded more mature bone tissue. Conclusion. Intraosseous injection of the oxygenated drug Perftoran in avascular femoral head at the os-teosynthesis of experimental hip fracture by bioactive titanium implants with calcium phosphate coating reduces inflammatory infiltration and secondary osteoresorption. This contributes to improving the remodeling of bone tissue and to the completion of the formation of mature bone fracture zone in the early stages compared to the group without the use of the drug Perftoran.