Cytomorphometric analysis on the effects of components of orthodontic appliances on the epithelial cells of the buccal mucosa

Orthodontic brackets release ions that can be reabsorbed in the oral mucosa, potentially causing complications, including cytotoxic effects and mutagenic alterations. The aim was to evaluate the genotoxicity induced by orthodontic appliance alloys in cultures of human gingival fibroblasts by comet assay. Eluates were obtained from the following brackets alloys: EconoLine (SS: stainless steel), MiniMirage (Ni-Ti: nickel-titanium), Nu-Edge (Co-Cr: cobalt-chromium), In-Vu (PC-polycrystals (PC) aluminum oxide), and Monocrystal IZE (monocrystalline (MC) aluminum oxide). Each bracket was sterilized and exposed to a corrosive process for 35 days. The obtained eluates were tested for genotoxicity of human gingival fibroblasts (HGFA) by the alkaline comet assay. All study groups showed genotoxic effects; there was a significant difference (p<0.0001) among groups. The eluates obtained from Ni-Ti showed a 16-times greater genotoxic effect. There were differences in genotoxicity after comparing the Ni-Ti with SS (p<0.01) and Co-Cr brackets (p<0.001). The ceramic was more genotoxic than metallic brackets (SS and Co-Cr), but less than the Ni-Ti. This in vitro model will be useful for further study of early DNA damage caused by brackets and other biomaterials used in the oral cavity before their introduction into the clinical setting.

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Genotoxic and cytotoxic effects of Haas appliance in exfoliated buccal mucosa cells during orthodontic treatment

Cunha

Castillo

Takahashi

et al. 2018

The Angle Orthodontist

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Cytomorphometric analysis on the effects of components of orthodontic appliances on the epithelial cells of the buccal mucosa

Cited by 3 publications

References 25 publications

Letter to the Editor replying to Armen Nersesyan about the article published in Clinical Oral Investigations tilted “Smoking increases the frequency of micronuclei in the oral mucosa of adults relative to non-smokers—a systematic review and meta-analysis”

Letter to the Editor replying to Armen Nersesyan about the article published in Clinical Oral Investigations tilted “Smoking increases the frequency of micronuclei in the oral mucosa of adults relative to non-smokers—a systematic review and meta-analysis”

In VitroDetermination of Genotoxicity Induced by Brackets Alloys in Cultures of Human Gingival Fibroblasts

Genotoxic and cytotoxic effects of Haas appliance in exfoliated buccal mucosa cells during orthodontic treatment

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