Cytometric Evaluation of Peripheral Blood Lymphocytes in Dogs with Lymphoma during Chemotherapy

Abstract

Twenty dogs with clinically diagnosed multicentric lymphoma were evaluated for percentages of peripheral blood lymphocyte subpopulations. Cytometric analysis was performed before and during chemotherapy. The results were compared to those obtained from a control group of healthy dogs. The percentages of CD5+, CD4+ and CD8+ cells were markedly decreased and CD21-like+ cells markedly increased in dogs with lymphoma in comparison with the control group. During the course of chemotherapy these values returned to ranges observed in healthy animals.

Regulatory T cells in dogs with multicentric lymphoma: peripheral blood quantification at diagnosis and after initial stage chemotherapy

“…O 'Neill et al (2009) The results of the present study show that the percentages of the subpopulations of CD4 + and CD8 + T-cells were not altered by chemotherapy and are in agreement with the results of other studies in dogs with lymphoma and multicentric lymphoma (Winnicka et al, 2002;Mitchell et al, 2012) and in dogs with osteosarcoma (Walter et al, 2006). Gauthier et al (2005) observed that chemotherapy caused a significant reduction in the absolute value of the majority of lymphocyte subpopulations of dogs with lymphoma.…”

“…Similar results have been previously reported by O'Neill et al (2009), who analysed dogs with various types of neoplasia (sarcoma, carcinoma, lymphoma and mastocytoma) and by Mitchell et al (2012) in dogs with type B lymphoma. On the other hand, other studies (Winnicka et al, 2002;Walter et al, 2006) have reported significant differences between healthy and neoplastic dogs and have attributed such differences to factors related to the tumours, suggesting that these tumours can be suppressive; however, no information on the type of suppression was provided (Winnicka et al, 2002). Some factors could have influenced this analysis and may explain some of the discrepancies in the results.…”

“…Some factors could have influenced this analysis and may explain some of the discrepancies in the results. Firstly, the way of expressing the results could be one of these factors since the relative values of CD4 + and CD8 + were transformed into absolute values (in relation to the number of lymphocytes obtained in the haemogram) (Winnicka et al, 2002). Another factor would be to analyse together data from different types of tumours, as described by O'Neill et al (2009).…”

“…As a matter of fact, Winnicka et al (2002) did not specify the immunophenotype of the lymphoma studied and Mitchell et al (2012) only included in their analysis immunophenotypes B. Therefore, the variations observed could be individual variations or related to the type of tumour being studied.…”

Lebensqualität und Lebenserwartung am malignen Lymphom erkrankter Hunde unter Chemotherapie

“…Bei den meisten mediastinalen und epidermotropen Lymphomen handelt es sich um T-ZellLymphome (MOORE et al, 1994;VAIL et al, 1996;RUSLANDER et al, 1997 (DUNCAN & PRASSE, 1979;CHUN et al, 2007;VAIL & YOUNG, 2007;DICKINSON, 2008 (CHABANNE et al, 2000a;CULMSEE et al, 2001). Diese Immuntypisierung kann sowohl mit Lymphknotenaspiraten als auch mit peripherem Blut durchgeführt werden (WINNICKA et al, 2002;MINISCALCO et al, 2003;GIBSON et al, 2004).…”