Cytometric analysis of blood cells from malaria‐infected patients and in vitro infected blood

Bunyaratvej, Ahnond; Butthep, Punnee; Bunyaratvej, Porntip

doi:10.1002/cyto.990140114

Cited by 12 publications

(8 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A high sensitivity (81%) and specificity (87%) was seen along with an increase in probability of malaria by factor 6.2, in concurrence with observations by other authors. 19 Although the role of RDW in the diagnosis of malaria is debatable, the presence of increased RDW has correlated well with the percentage of macrocytes in one study, 30 hence a combination of RDW and MCV may be more helpful. 28 Dhungat et al concluded that although a reliable diagnostic marker, there is no prognostic significance of thrombocytopenia in malarial fevers.…”

Section: Discussionmentioning

confidence: 96%

An Analysis of Hematological Parameters as a Diagnostic test for Malaria in Patients with Acute Febrile Illness: An Institutional Experience

et al. 2014

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 96%

An Analysis of Hematological Parameters as a Diagnostic test for Malaria in Patients with Acute Febrile Illness: An Institutional Experience

et al. 2014

View full text Add to dashboard Cite

show abstract

“…In 1993, a study analysing 18 samples from patients with malaria ( P. falciparum: 10 and P. vivax : 8) and 52 samples from healthy controls with a Technicon H1 ® analyser (Technicon Instruments Corporation, Tarry Town, NY; now Siemens), found that all malaria-infected patients had ≥3% (range 3.3-20.9%) of so-called 'large-unstained-cells' suggesting their potential use for malaria screening [25]. Unfortunately, changes in WBC populations [26], reticulocytosis, or increase of 'large-unstained-cells' [25] may also appear with other pathologies, giving these changes low accuracy for malaria detection.…”

Section: Discovery Of Automated Haematology Analysers For Malaria Diamentioning

confidence: 99%

Automated haematology analysis to diagnose malaria

Campuzano-Zuluaga

Hänscheid

Grobusch

2010

Malar J

View full text Add to dashboard Cite

For more than a decade, flow cytometry-based automated haematology analysers have been studied for malaria diagnosis. Although current haematology analysers are not specifically designed to detect malaria-related abnormalities, most studies have found sensitivities that comply with WHO malaria-diagnostic guidelines, i.e. ≥ 95% in samples with > 100 parasites/μl. Establishing a correct and early malaria diagnosis is a prerequisite for an adequate treatment and to minimizing adverse outcomes. Expert light microscopy remains the 'gold standard' for malaria diagnosis in most clinical settings. However, it requires an explicit request from clinicians and has variable accuracy. Malaria diagnosis with flow cytometry-based haematology analysers could become an important adjuvant diagnostic tool in the routine laboratory work-up of febrile patients in or returning from malaria-endemic regions. Haematology analysers so far studied for malaria diagnosis are the Cell-Dyn®, Coulter® GEN·S and LH 750, and the Sysmex XE-2100® analysers. For Cell-Dyn analysers, abnormal depolarization events mainly in the lobularity/granularity and other scatter-plots, and various reticulocyte abnormalities have shown overall sensitivities and specificities of 49% to 97% and 61% to 100%, respectively. For the Coulter analysers, a 'malaria factor' using the monocyte and lymphocyte size standard deviations obtained by impedance detection has shown overall sensitivities and specificities of 82% to 98% and 72% to 94%, respectively. For the XE-2100, abnormal patterns in the DIFF, WBC/BASO, and RET-EXT scatter-plots, and pseudoeosinophilia and other abnormal haematological variables have been described, and multivariate diagnostic models have been designed with overall sensitivities and specificities of 86% to 97% and 81% to 98%, respectively. The accuracy for malaria diagnosis may vary according to species, parasite load, immunity and clinical context where the method is applied. Future developments in new haematology analysers such as considerably simplified, robust and inexpensive devices for malaria detection fitted with an automatically generated alert could improve the detection capacity of these instruments and potentially expand their clinical utility in malaria diagnosis.

show abstract

“…The same mechanism however cannot be said to be associated with RDW increase in P. falciparum infection since parasitized cells maintain their sizes [29]. The elevated RDW seen in this study could result from the bone marrow’s effort to balance erythrocytes loss in malaria by pushing more new erythrocytes into circulation which also increases the macrocyte percentage [30, 31]. …”

Section: Discussionmentioning

confidence: 93%

Proteomic analysis of microparticles isolated from malaria positive blood samples

et al. 2016

View full text Add to dashboard Cite

BackgroundMalaria continues to be a great public health concern due to the significant mortality and morbidity associated with the disease especially in developing countries. Microparticles (MPs), also called plasma membrane derived extracellular vesicles (PMEVs) are subcellular structures that are generated when they bud off the plasma membrane. They can be found in healthy individuals but the numbers tend to increase in pathological conditions including malaria. Although, various studies have been carried out on the protein content of specific cellular derived MPs, there seems to be paucity of information on the protein content of circulating MPs in malaria and their association with the various signs and symptoms of the disease. The aim of this study was therefore to carry out proteomic analyses of MPs isolated from malaria positive samples and compare them with proteins of MPs from malaria parasite culture supernatant and healthy controls in order to ascertain the role of MPs in malaria infection.MethodsPlasma samples were obtained from forty-three (43) malaria diagnosed patients (cases) and ten (10) healthy individuals (controls). Malaria parasite culture supernatant was obtained from our laboratory and MPs were isolated from them and confirmed using flow cytometry. 2D LC-MS was done to obtain their protein content. Resultant data were analyzed using SPSS Ver. 21.0 statistical software, Kruskal Wallis test and Spearman’s correlation coefficient r.ResultsIn all, 1806 proteins were isolated from the samples. The MPs from malaria positive samples recorded 1729 proteins, those from culture supernatant were 333 while the control samples recorded 234 proteins. The mean number of proteins in MPs of malaria positive samples was significantly higher than that in the control samples. Significantly, higher quantities of haemoglobin subunits were seen in MPs from malaria samples and culture supernatant compared to control samples.ConclusionA great number of proteins were observed to be carried in the microparticles (MPs) from malaria samples and culture supernatant compared to controls. The greater loss of haemoglobin from erythrocytes via MPs from malaria patients could serve as the initiation and progression of anaemia in P.falciparum infection. Also while some proteins were upregulated in circulating MPs in malaria samples, others were down regulated.

show abstract

Cytometric analysis of blood cells from malaria‐infected patients and in vitro infected blood

Cited by 12 publications

References 14 publications

An Analysis of Hematological Parameters as a Diagnostic test for Malaria in Patients with Acute Febrile Illness: An Institutional Experience

An Analysis of Hematological Parameters as a Diagnostic test for Malaria in Patients with Acute Febrile Illness: An Institutional Experience

Automated haematology analysis to diagnose malaria

Proteomic analysis of microparticles isolated from malaria positive blood samples

Contact Info

Product

Resources

About