Cytology of the Secretion in Mammalian Sweat Glands

Kurosumi, Kazumasa; Shibasaki, Susumu; Ito, Toshio

doi:10.1016/s0074-7696(08)62445-6

Cited by 77 publications

(47 citation statements)

References 79 publications

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“…It has been shown that the apocrine portion of the infraorbital gland of the Japanese serow secreted large amounts of glycoconjugates that were released from secretory cells (Atoji & Suzuki 1990). The findings contrast with a 376 T. Rajagopal & G. Archunan previous study by Kurosumi et al (1984) who reported that there was no apocrine secretion in the dorsal skin of the dog.…”

Section: Discussioncontrasting

confidence: 96%

Histomorphology of preorbital gland in territorial and non-territorial male blackbuck Antelope cervicapra, a critically endangered species

Rajagopal

Archunan

2011

Biologia

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Abstract:The preorbital gland is a specialized dermal gland of antelopes which plays an important role in territorial marking behavior and pheromonal communication. To our knowledge, there is little information available on the role of preorbital gland marks in Indian antelopes (blackbucks). Males are seen averting the gland during behavioural display and territorial marking but the functional aspect of this gland has not been examined. Hence, the aim of this study was to describe the histomorphology of the preorbital gland in territorial and non-territorial male blackbucks to determine its morphology and secretory function. The results showed that the preorbital gland is composed of modified sebaceous and apocrine glands. The apocrine gland is lined by simple cuboidal epithelial cells; the serous parts of the secretory products are often seen in the apical portions of the cells. The myoepithelial cells contain actin filaments lying on the basal membranes of the apocrine gland. There are some considerable histological changes in the presence of the sebaceous and apocrine glands in territorial males in comparison to non-territorial males. The following histological changes associated with occurrence of the sebaceous and apocrine glands have been observed in territorial and non-territorial male blackbucks: (1) increase of size of sebaceous and apocrine glands and (2) increase in density of sebaceous and apocrine glands in territorial males compared to non-territorial males. It is suggested that the higher development (i.e., size) and density of sebaceous and apocrine glands in territorial males could depend on hormone production (i.e., testosterone). Based on the histological observation and the role of sebaceous and apocrine glands in the preorbital gland supported by literature, it is possible to conclude that both territorial and non-territorial blackbuck males may produce pheromonal substances through preorbital gland (secretion) for olfactory communication.

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Section: Discussioncontrasting

confidence: 96%

Histomorphology of preorbital gland in territorial and non-territorial male blackbuck Antelope cervicapra, a critically endangered species

Rajagopal

Archunan

2011

Biologia

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“…The walls of the secretory tubules are composed ofglandular, secretory cells, and contractile, myoepithelial cells. The glandular cells are comprised of a simple epithelium and are surrounded by the myoepithelial cells (11). Fig.…”

Section: Resultsmentioning

confidence: 99%

Monoclonal antibody that defines human myoepithelium.

Dairkee¹,

Blayney²,

Smith³

et al. 1985

Proc. Natl. Acad. Sci. U.S.A.

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We have isolated a mouse monoclonal antibody that, upon immunohistochemical localization in frozen sections, displays specificity for human myoepithelial cells in the resting mammary gland, sweat glands, and salivary glands. Furthermore, this antibody was strongly and homogeneously reactive with frozen sections of 3 of 60 breast carcinoma specimens. Using immunolocalization techniques in conjunction with polyacrylamide gel electrophoresis, we have determined that the reactivity of this monoclonal antibody is directed toward a 51,000-dalton keratin polypeptide. The potential uses of this antibody in the prognosis of human mammary carcinoma and in understanding the role of the myoepithelium in development and differentiation are discussed.Myoepithelial cells are derived from ectoderm and are known to exhibit both epithelial and mesenchymal characteristics (1, 2). They are situated between acinar or ductal luminal cells and the basal lamina in a number of secretory glands such as breast, lacrimal, eccrine, and apocrine sweat glands and various salivary glands (3).We report here on a monoclonal antibody directed toward a cytoskeletal keratin that is unique to the myoepithelial or "basal" layer of epithelial cells in human mammary ducts, sweat glands, and salivary glands. Using polyacrylamide gel electrophoresis and immunoblotting, we have found the apparent molecular weight of this basal epithelium-specific keratin to be 51,000 daltons. The antibody is generally nonreactive with luminal epithelium, stroma, muscle, fat, and vasculature. Therefore, it appears to be a useful tool in studying the role of the myoepithelium in the normal development of the breast, during lactation and involution, and in malignancy and metastasis.Recently, considerable advances have been made in tumor diagnosis by immunocytochemical typing of intermediate filaments. For example, carcinomas are characterized by antibodies to cytokeratins; sarcomas of muscle cells, by anti-desmin; nonmuscle sarcomas, by anti-vimentin; and gliomas, by anti-glial fibrillary acidic protein (for review, see ref. 4). The myoepithelium-specific monoclonal antibody described in this report has allowed us to define and characterize subclasses of human mammary carcinomas that would not be possible to distinguish by conventional techniques. The potential application of this antibody in the prognosis of human mammary carcinoma is discussed. MATERIALS AND METHODSImmunization. BALB/c mice raised under pathogen-free conditions were injected via subcutaneous or intravenous routes for 5 weeks at weekly intervals with whole-cell preparations of a secondary culture of an infiltrating ductal breast carcinoma. For each inoculation, 106 cells, which had been cultured as previously described (5), were prepared by scraping the monolayer with a rubber policeman into phosphate-buffered saline. Splenocytes from immunized animals were fused with SP2/0 mouse myeloma cells in the presence of polyethylene glycol (Mr 4000; Merck) by the procedure of Kohler and Milstein (6). Monocl...

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“…The secretory segment and the excretory duct have been well investigated with the electron microscope (for reviews see ELLIS, 1967, andKUROSUMI et al, 1984). However, only a few studies have briefly described the ultrastructure of the transitional portion in human eccrine sweat glands (HIBBS, 1958;SHIBASAKI and ITO, 1967;HASHIMOTO, 1978;KUROSUMI et al, 1982;MONTGOMERY et al, 1985), arising from the difficulty in detecting short part in the routine observations of the gland.…”

mentioning

confidence: 99%

“…For convenience in describing various cell types in the secretoty segment and the duct, the terminology recommended by KuRosuMI et al (1984) was used in this study.…”

mentioning

confidence: 99%