Cytology of the Normal Endometrium – Cycling and Postmenopausal

Jiménez-Ayala, Matías; Portillo, Beatriz Jiménez-Ayala

doi:10.1159/000117494

Cited by 9 publications

(14 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…By longitudinally profiling the accumulation of fibrosis across the FRT during aging, we discovered that the scale of ECM remodeling found in each organ during the cycle closely predicts fibrosis development in old age. Previous studies reported evidence of fibrosis development in post-menopausal endometrium (Jiménez-Ayala and Jiménez-Ayala, 2008; Noci et al, 1996); our data newly reveals that this fibrosis accumulates steadily across aging and that the age-related decrease in hormonal stimulation is not the primary driver of fibrosis development.…”

Section: Discussionsupporting

confidence: 65%

The function and decline of the female reproductive tract at single-cell resolution

Winkler

Tolkachov

Lammers

et al. 2022

Preprint

View full text Add to dashboard Cite

The female reproductive tract (FRT) undergoes extensive remodeling during each reproductive cycle, regulated by systemic changes in sex hormones. Whether this recurrent remodeling influences a specific organ's aging trajectory is unknown. To address this, we systematically characterized at single-cell resolution the morphological and transcriptional changes that occur in ovary, oviduct, uterus, cervix, and vagina at each phase of the mouse estrus cycle, during decidualization, and into aging. Transcriptional and cell-to-cell communication networks in estrus cycle and aging are enriched for ECM reorganization and inflammation, two essential components of FRT remodeling. We directly link the organ-specific level of these two processes over reproductive lifespan with the gradual, age-related development of fibrosis and chronic inflammation. Our data represent a comprehensive atlas of the FRT lifespan, revealing pathological consequences of incomplete resolution of recurrent inflammation and tissue repair.

show abstract

Section: Discussionsupporting

confidence: 65%

The function and decline of the female reproductive tract at single-cell resolution

Winkler

Tolkachov

Lammers

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…This cycling is accompanied by dramatic morphological changes in the endometrial glands. However, despite these changes, the glandular epithelial cells maintain normal apical-basal polarity through specific subcellular expression of polarity proteins 16 . Loss of cellular polarity allows cells to override normal contact inhibition signals and is thus a hallmark of cancer 13 .…”

Section: Discussionmentioning

confidence: 99%

CTCF genetic alterations in endometrial carcinoma are pro-tumorigenic

et al. 2017

View full text Add to dashboard Cite

CTCF is a haploinsufficient tumour suppressor gene with diverse normal functions in genome structure and gene regulation. However the mechanism by which CTCF haploinsufficiency contributes to cancer development is not well understood. CTCF is frequently mutated in endometrial cancer. Here we show that most CTCF mutations effectively result in CTCF haploinsufficiency through nonsense mediated decay of mutant transcripts, or loss-of-function missense mutation. Conversely, we identified a recurrent CTCF mutation K365T, which alters a DNA binding residue, and acts as a gain-of-function mutation enhancing cell survival. CTCF genetic deletion occurs predominantly in poor prognosis serous subtype tumours, and this genetic deletion is associated with poor overall survival. In addition, we have shown that CTCF haploinsufficiency also occurs in poor prognosis endometrial clear cell carcinomas and has some association with endometrial cancer relapse and metastasis. Using shRNA targeting CTCF to recapitulate CTCF haploinsufficiency, we have identified a novel role for CTCF in the regulation of cellular polarity of endometrial glandular epithelium. Overall, we have identified two novel pro-tumorigenic roles (promoting cell survival and altering cell polarity) for genetic alterations of CTCF in endometrial cancer.

show abstract

“…Class I HDACs [1][2][3]8] are critical for modulating cell survival and proliferation, and HDACs 1, 2, and 3 play an important role in steroid hormone-dependent gene expression in the human endometrium [9,10]. A previous study on human cyclic endometrial tissues has demonstrated that the global acetylation levels of H2AK5, H3K9, and H4K8 are elevated in the early proliferative phase, and are associated with the transcriptional activation of proliferative and regenerative pathways [6].…”

Section: Proliferationmentioning

confidence: 99%

“…Its fundamental function is to provide an immunoprivileged site for embryo implantation and a nurturing environment for fetal development [1]. It mainly consists of luminal epithelium, glandular epithelium, and endometrial stromal cells; which undergo regeneration, proliferation, differentiation, and degradation under the influence of steroid hormones estrogen and progesterone [1][2][3][4]. There are several genes involved in cyclic morphological and functional changes in the endometrium.…”

Section: Introductionmentioning

confidence: 99%

Histone acetylation and the role of histone deacetylases in normal cyclic endometrium

Gujral

Mahajan

Lissaman

et al. 2020

Reprod Biol Endocrinol

108

View full text Add to dashboard Cite

Histone acetylation is a critical epigenetic modification that changes chromatin architecture and regulates gene expression by opening or closing the chromatin structure. It plays an essential role in cell cycle progression and differentiation. The human endometrium goes through cycles of regeneration, proliferation, differentiation, and degradation each month; each phase requiring strict epigenetic regulation for the proper functioning of the endometrium. Aberrant histone acetylation and alterations in levels of two acetylation modulators-histone acetylases (HATs) and histone deacetylases (HDACs)-have been associated with endometrial pathologies such as endometrial cancer, implantation failures, and endometriosis. Thus, histone acetylation is likely to have an essential role in the regulation of endometrial remodelling throughout the menstrual cycle.

show abstract

Cytology of the Normal Endometrium – Cycling and Postmenopausal

Cited by 9 publications

References 0 publications

The function and decline of the female reproductive tract at single-cell resolution

The function and decline of the female reproductive tract at single-cell resolution

CTCF genetic alterations in endometrial carcinoma are pro-tumorigenic

Histone acetylation and the role of histone deacetylases in normal cyclic endometrium

Contact Info

Product

Resources

About