Cytology and digoxin production in hybrids between Digitalis lanata and D. grandiflora

Kennedy, A. J.

doi:10.1007/bf00039142

Cited by 4 publications

(4 citation statements)

References 1 publication

(1 reference statement)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition to the expected cardiac glycosides, steroidal saponins as well as pregnane and furostanol glycosides were also present in the analyzed extracts. A detailed LC/MS qualitative analysis revealed very similar profiles of steroidal glycosides in the extracts of D. lanata and D. ferruginea leaves, in particular of digoxin, deslanoside, and lanatosides A, B, and C, which is consistent to earlier reports ( Kennedy, 1978 ; Braga et al., 1997 ; Verma et al., 2014 ; Yücesan et al., 2018 ). On the other hand, these compounds were not detected in leaves of D. grandiflora although digoxin has been reported in small amounts in an earlier study ( Kennedy, 1978 ).…”

Section: Resultssupporting

confidence: 90%

“…A detailed LC/MS qualitative analysis revealed very similar profiles of steroidal glycosides in the extracts of D. lanata and D. ferruginea leaves, in particular of digoxin, deslanoside, and lanatosides A, B, and C, which is consistent to earlier reports (Kennedy, 1978;Braga et al, 1997;Verma et al, 2014;Yücesan et al, 2018). On the other hand, these compounds were not detected in leaves of D. grandiflora although digoxin has been reported in small amounts in an earlier study (Kennedy, 1978). Table 2 specifies which of the identified compounds were previously detected in any Digitalis species, while for a certain number of compounds it can be concluded that they were identified for the first time in Digitalis species.…”

Section: Species-specific Metabolite Profilessupporting

confidence: 91%

“…are both widely collected from nature and field-cultivated to extract cardenolides, being among the best producers of these compounds in the plant world ( Clemente et al., 2011 ). One of them, digoxin, is mainly produced in Europe from dried leaves of D. lanata , reaching up to 1.5% dry weight ( Kennedy, 1978 ; Bown, 1995 ; Clemente et al., 2011 ). There is a vast diversity of cardenolides within the genus Digitalis with more than one hundred different compounds isolated, the most commercially attractive, besides digoxin, being lanatoside C, digitoxin, and acetyldigitoxin ( Clemente et al., 2011 ; Kreis, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

“…Among these, D. lanata is somewhat better studied in terms of genetic and phenetic diversity and by its potential to accumulate various specialized metabolites ( Braga et al., 1997 ; Yücesan et al., 2018 ). Few data are available for the other species listed ( Kennedy, 1978 ; Katanić et al., 2017 ; Kaska et al., 2020 ). Surprisingly, variation in metabolite fingerprints both between and within wild populations is pretty much neglected, having in mind that it can render starting material for selective breeding or at least for the selection of specific genotypes that would accumulate metabolite amounts appropriate for the pharmaceutical industry.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Variation in the chemical profiles of three foxglove species in the central Balkans

et al. 2023

View full text Add to dashboard Cite

The aim of this study was to determine intra- and interspecies variation in the qualitative and quantitative composition of methanol-soluble metabolites in the leaves of three Digitalis species (D. lanata, D. ferruginea, and D. grandiflora) from the central Balkans. Despite the steady use of foxglove constituents for human health as valuable medicinal products, populations of the genus Digitalis (Plantaginaceae) have been poorly investigated to describe their genetic and phenetic variation. Following untargeted profiling using UHPLC-LTQ Orbitrap MS, by which we identified a total of 115 compounds, 16 compounds were quantified using the UHPLC(–)HESI–QqQ-MS/MS approach. In total, 55 steroid compounds, 15 phenylethanoid glycosides, 27 flavonoids, and 14 phenolic acid derivatives were identified across the samples with D. lanata and D. ferruginea showing a great similarity, while 15 compounds were characteristic only for D. grandiflora. The phytochemical composition of methanol extracts, considered here as complex phenotypes, are further examined along multiple levels of biological organization (intra- and interpopulation) and subsequently subjected to chemometric data analysis. The quantitative composition of the selected set of 16 chemomarkers belonging to the classes of cardenolides (3 compounds) and phenolics (13 compounds) pointed to considerable differences between the taxa studied. D. grandiflora and D. ferruginea were found to be richer in phenolics as compared to cardenolides, which otherwise predominate in D. lanata over other compounds. PCA revealed lanatoside C, deslanoside, hispidulin, and p-coumaric acid to be the main compounds contributing to the differences between D. lanata on one side and D. grandiflora and D. ferruginea on the other, while p-coumaric acid, hispidulin, and digoxin contribute to the diversification between D. grandiflora and D. ferruginea. However, quantitative variation in the metabolite content within species was faint with mild population diversification visible in D. grandiflora and particularly in D. ferruginea. This pointed to the highly conserved content and ratio of targeted compounds within the analyzed species, which was not severely influenced by the geographic origin or environmental conditions. The presented metabolomics approach might have, along with morphometrics and molecular genetics studies, a high information value for further elucidation of the relationships among taxa within the genus Digitalis.

show abstract