Cytological Evidence Supporting the Inclusion of Poplar Mosaic Virus in the Carlavirus Group of Plant Viruses

Brunt, A. A.; Stace-Smith, R.; Leung, Esther

doi:10.1159/000149962

Cited by 16 publications

(11 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Properties and morphology of its particles (Biddle and Tinsley, 1971a;Boccardo and Milne, 1976) place PMV in the carlavirus group (Fenner, 1976), although aphid transmission is uncertain, and serological relationships with recognized members of the carlavirus group could not be detected (Biddle and Tinsley, 1971c). Electron-microscopical studies of the virus in ultra-thin sections also indicate that PMV belongs to the carlavirus group (Atkinson and Cooper, 1976;Brunt et al, 1976;Boccardo and Milne, 1976).…”

Section: Introductionmentioning

confidence: 99%

Poplar mosaic virus: purification, antiserum preparation, and detection in poplars with the enzyme-linked immunosorbent assay (ELISA) and with infectivity tests on Nicotiana megalosiphon

Meer¹,

Maat²,

Vink³

1980

Netherlands Journal of Plant Pathology

View full text Add to dashboard Cite

Poplar mosaic virus (PMV) was purified from Nicotiana megalosiphon or N. clevelandii and antisera with titres from 256 to 4096 were prepared. One of these was used for the detection of PMV in poplar stools with the enzyme-linked immunosorbent assay (ELISA). Although ELISA was less sensitive than the infectivity test on N. megalosiphon, both tests were equally reliable when using lower leaves in July and August, and when applied to tip leaves at the end of the growing season, With both tests more infected trees were detected than with visual inspection. For PMV a particle length of 661 nm was calculated. AddressInstituut voor Plantenziektenkundig Onderzoek, Binnenhaven 12, Postbus 42, 6700 AA Wageningen.

show abstract

Section: Introductionmentioning

confidence: 99%

Poplar mosaic virus: purification, antiserum preparation, and detection in poplars with the enzyme-linked immunosorbent assay (ELISA) and with infectivity tests on Nicotiana megalosiphon

Meer¹,

Maat²,

Vink³

1980

Netherlands Journal of Plant Pathology

View full text Add to dashboard Cite

show abstract

“…We have investi gated, therefore, the extent to which the in vivo occurrence of CMMV resembles and differs from that of recognized members of the Carlavirus group [12,13]. We describe here its intracellular occurrence in two unrelated plant species and discuss these observations in rela tion to the taxonomy of the virus.…”

mentioning

confidence: 95%

“…Because allied plant viruses usually induce similar cytopathological effects in their hosts, cytological investigations can sometimes in dicate the affinities of viruses of uncertain taxonomic status [11,12]. We have investi gated, therefore, the extent to which the in vivo occurrence of CMMV resembles and differs from that of recognized members of the Carlavirus group [12,13].…”

mentioning

confidence: 99%

Intracellular Occurrence of Cowpea Mild Mottle Virus in Two Unrelated Plant Species

Brunt¹,

Atkey²,

Woods³

1983

Intervirology

Self Cite

View full text Add to dashboard Cite

The filamentous particles of cowpea mild mottle virus (CMMV) were detected only in the cytoplasm of palisade, mesophyll, parenchyma and epidermal cells of Glycine max (Papilionaceae) and Nicotiana develandii (Solanaceae). They were aggregated to form either sheets or bundles or, more frequently, brush-like inclusions. Although CMMV was initially considered to be a possible member of the Carlavirus group, it differs from aphid-borne carla-viruses in its intracellular occurrence and transmission by whiteflies and in being frequently seed-transmitted in some leguminous hosts. Because CMMV is also serologically unrelated to any of 12 recognized carlaviruses, we suggest that it now be removed from the carlavirus group and left unclassified until the taxonomic significance of these differences has been fully evaluated.

show abstract

“…Nevertheless, to have a biological significance, this interaction, shown in experimental conditions, must also be demonstrated within tobacco plant as well as using other PVY strains and other host plants. Despite the fact that some members of the Potyvirus genus induce structural changes in chloroplasts (Brunt et al, 1976;Fraser et al, 1979;Russo et al, 1987), only one interaction of a chloroplast protein and a member of this genus has been reported. This interaction involves a 37 kDa chloroplast protein and the TMV-CP (McClintock et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

Interaction between tobacco Ribulose-l,5-biphosphate Carboxylase/Oxygenase large subunit (RubisCO-LSU) and the PVY Coat Protein (PVY-CP)

et al. 2005

View full text Add to dashboard Cite

A 54 kDa band (P54) was continually detected with the 30 kDa viral capsid protein (CP) on the SDS-PAGE migration profile of purified potato virus Y (PVY). P54 was observed following the use of two different procedures for the purification of the PVY from infected tobacco. It was a constitutively expressed tobacco protein. The analysis of the PVY preparation showed that P54 has aggregation properties and precipitates, thus pulling down the virus. We used an enzyme-linked immunosorbent assay (ELISA) to study the relationship between P54 and the PVY particles. We performed an inhibition test with monoclonal antibodies (mAb) directed against the PVY-CP, to show that these two components interact. This result was confirmed by western blot. The internal sequence of five major peptides, obtained by C-lysine endoprotease digestion of the P54 followed by HPLC separation, showed 100% homology with the large subunit of the ribulose-1,5-biphosphate carboxylase/oxygenase (RubisCO-LSU) of tobacco. MAb directed against RubisCO-LSU were produced and used to reveal the RubisCO-LSU/PVY complex in infected tobacco extracts. A phage library displaying random heptapeptides was used to isolate several peptides that specifically bound to the native form of the PVY. The sequences of thirty-three phage-displayed peptides, which bound specifically to this virus, present further discontinuous sequence homologies with the RubisCO-LSU. Five peptides (p1 to p5) corresponding to the RubisCO-LSU homologous regions were used for a bacterial two-hybrid system to confirm in vivo direct interactions between the selected RubisCO-LSU regions and the PVY-CP. We propose that the PVY-CP may be involved in the production of mosaics and yellowing symptoms in tobacco through its interaction with RubisCO-LSU. Abbreviations: AC

show abstract

Cytological Evidence Supporting the Inclusion of Poplar Mosaic Virus in the Carlavirus Group of Plant Viruses

Cited by 16 publications

References 8 publications

Poplar mosaic virus: purification, antiserum preparation, and detection in poplars with the enzyme-linked immunosorbent assay (ELISA) and with infectivity tests on Nicotiana megalosiphon

Poplar mosaic virus: purification, antiserum preparation, and detection in poplars with the enzyme-linked immunosorbent assay (ELISA) and with infectivity tests on Nicotiana megalosiphon

Intracellular Occurrence of Cowpea Mild Mottle Virus in Two Unrelated Plant Species

Interaction between tobacco Ribulose-l,5-biphosphate Carboxylase/Oxygenase large subunit (RubisCO-LSU) and the PVY Coat Protein (PVY-CP)

Contact Info

Product

Resources

About