Cytological analysis of mycelial incompatibility in Rosellinia necatrix

Inoue, Kanako; Kanematsu, Satoko; Park, Pyoyun; Ikeda, Kenichi

doi:10.1016/j.funbio.2010.10.009

Cited by 16 publications

(23 citation statements)

References 40 publications

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“…Heterogenic incompatibility in R. necatrix results in a lack of anastomosis (12). We evaluated the dynamics of the cytosol during heterogenic incompatibility by using a green fluorescent protein (GFP)-expressing strain.…”

Section: Resultsmentioning

confidence: 99%

“…The observation of hyphal contact was described previously (12). R. necatrix strains were paired on cellulose membranes (1.5-cm square) laid onto 1/10-strength OMA plates with or without zinc compounds (0.5 to 1 mM ZnCl 2 and ZnSO 4 ) in 50-mm-diameter petri dishes (AS One, Osaka, Japan).…”

Section: Figmentioning

confidence: 99%

“…Membranes with mycelia were transferred onto a glass slide overlaid with 1/10-strength OMA with or without zinc compounds and incubated for 1 day at 25°C in 90-mm-diameter petri dishes that contained agar medium (15 g/liter agar) to retain moisture. Specimen preparation and ultrathin sectioning for transmission electron microscopy (TEM) were performed according to the method of Inoue et al (12). The sections were observed by using a JEM-1400 electron microscope (JEOL, Akishima, Japan) at an accelerating voltage of 80 kV.…”

Section: Figmentioning

confidence: 99%

“…Although this phenomenon is commonplace in filamentous fungi, an absence of hyphal anastomosis has also been reported in some fungi (8,9,10,11). For R. necatrix, an ultrastructural study revealed that heterogenic incompatibility occurred without anastomosis, and the mechanism involved was shown to be a novel vacuole-mediated type of PCD (12).…”

mentioning

confidence: 95%

“…However, this fungal system frequently anastomoses in incompatible combinations (13). In contrast, R. necatrix showed no opportunity for transmission of mycoviruses because the incompatible hyphae hardly anastomosed (12). Although heterogenic incompatibility genes in R. necatrix were not characterized, these genes seem to be variable because of the following observations.…”

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confidence: 98%

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Potentiation of Mycovirus Transmission by Zinc Compounds via Attenuation of Heterogenic Incompatibility in Rosellinia necatrix

Ikeda

Inoue

Kida

et al. 2013

Appl Environ Microbiol

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cHeterogenic incompatibility is considered a defense mechanism against deleterious intruders such as mycovirus. Rosellinia necatrix shows strong heterogenic incompatibility. In the heterogenic incompatibility reaction, the approaching hyphae hardly anastomosed, a distinctive barrage line formed, and green fluorescent protein (GFP)-labeled hyphae quickly lost their fluorescence when encountering incompatible hyphae. In this study, transmission of a hypovirulence-conferring mycovirus to strains with different genetic backgrounds was attempted. Various chemical reagents considered to affect the programmed cell death pathway or cell wall modification were examined. Treatment with zinc compounds was shown to aid in transmission of mycoviruses to strains with different genetic backgrounds. In incompatible pairings, treatment with zinc compounds accelerated hyphal anastomosis; moreover, cytosolic GFP was transmitted to the newly joined hyphae. These results suggest that zinc compounds not only increase hyphal anastomosis but also attenuate heterogenic incompatibility.

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Section: Resultsmentioning

confidence: 99%

Section: Figmentioning

confidence: 99%

Section: Figmentioning

confidence: 99%

mentioning

confidence: 95%

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confidence: 98%

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