Cytological analysis in embryogenic callus cultures and regenerated plants of Papaver somniferum L. (opium poppy).

Wakhlu, A. K.; Bajwa, P. S.

doi:10.1508/cytologia.52.631

Cited by 8 publications

(6 citation statements)

References 15 publications

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“…4 c,f). This was similar to previous genome size estimates and supports reports that P. setigerum is tetraploid (2n = 44) with chromosomes smaller in size compared with the diploid (2n = 22) P. somniferum 33 – 35 . PAP 400 had a slightly smaller genome size than the diploid P. somniferum (Fig.…”

Section: Resultssupporting

confidence: 92%

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Insights into opium poppy (Papaver spp.) genetic diversity from genotyping-by-sequencing analysis

Hong

Tamiru‐Oli

Hurgobin

et al. 2022

Sci Rep

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Opium poppy (Papaver somniferum) is one of the world’s oldest medicinal plants and a versatile model system to study secondary metabolism. However, our knowledge of its genetic diversity is limited, restricting utilization of the available germplasm for research and crop improvement. We used genotyping-by-sequencing to investigate the extent of genetic diversity and population structure in a collection of poppy germplasm consisting of 91 accessions originating in 30 countries of Europe, North Africa, America, and Asia. We identified five genetically distinct subpopulations using discriminate analysis of principal components and STRUCTURE analysis. Most accessions obtained from the same country were grouped together within subpopulations, likely a consequence of the restriction on movement of poppy germplasm. Alkaloid profiles of accessions were highly diverse, with morphine being dominant. Phylogenetic analysis identified genetic groups that were largely consistent with the subpopulations detected and that could be differentiated broadly based on traits such as number of branches and seed weight. These accessions and the associated genotypic data are valuable resources for further genetic diversity analysis, which could include definition of poppy core sets to facilitate genebank management and use of the diversity for genetic improvement of this valuable crop.

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Section: Resultssupporting

confidence: 92%

“…Our result based on genome size analysis supports previous reports that P. somniferum is diploid (2n = 22) and P. setigerum tetraploid (2n = 44) (Fig. 4 ) 34 , 35 . These data do not seem to support the hypothesis of a direct origin of P. somniferum from P. setigerum .…”

Section: Discussionsupporting

confidence: 91%

Insights into opium poppy (Papaver spp.) genetic diversity from genotyping-by-sequencing analysis

Hong

Tamiru‐Oli

Hurgobin

et al. 2022

Sci Rep

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show abstract

“…4c , f ). This was similar to previous genome size estimates and supports reports that P. setigerum is tetraploid (2n = 4× = 44) with chromosomes smaller in size compared with the diploid (2n = 2× = 22) P. somniferum (Malik et al ., 1979; Wakhlu & Bajwa, 1987; Srivastava & Lavania, 1991). PAP 400 had a slightly smaller genome size than the diploid P. somniferum (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Our result based on genome size analysis supports previous reports that P. somniferum is diploid (2n = 2× = 22) and P. setigerum tetraploid (2n = 4× = 44) (Fig. 4 ) (Malik et al ., 1979; Wakhlu & Bajwa, 1987). These data do not support the hypothesis of a direct origin of P. somniferum from P. setigerum .…”

Section: Discussionmentioning

confidence: 99%

Insights into opium poppy (Papaver spp.) genetic diversity from genotyping-by-sequencing analysis

Hong

Tamiru‐Oli

Hurgobin

et al. 2021

Preprint

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Opium poppy (Papaver somniferum) is one of the world's oldest medicinal plants and a versatile model system to study secondary metabolism. However, our knowledge of its genetic diversity is limited, restricting utilization of the available germplasm for research and crop improvement. We used genotyping-by-sequencing to investigate the level of genetic diversity and population structure in a collection of poppy germplasm consisting of 91 accessions originating in 30 countries of Europe, North Africa, America, and Asia. We identified five genetically distinct subpopulations. Although the accessions were from geographically diverse regions, no strong association was detected between geographic origin and subpopulation at regional and sub-regional levels. However, most accessions obtained from the same country were grouped together as genetically distinct, likely a consequence of the restriction on movement of poppy germplasm. Phylogenetic analysis identified clades that were largely consistent with the subpopulations detected. Clades could be differentiated broadly based on capsule dehiscence, plant stature and seed weight, traits believed to be associated with poppy domestication. Our study determined that the genetically diverse collection was likely composed of both wild and cultivated forms. This has direct implications for germplasm management and utilization of the available diversity for genetic improvement of poppy.

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“…e.g. Swedlund and Vasil, 1985;Wakhlu and Bajwa, 1987). Historically, this had its origin in the view that adventitious buds are multicellular and hence potentially chimera!…”

Section: Somatic Embryos and Somaclonal Variationmentioning

confidence: 99%

Historical Insights into Some Contemporary Problems in Somatic Embryogenesis

Krikorian

2000

Forestry Sciences

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Cytological analysis in embryogenic callus cultures and regenerated plants of Papaver somniferum L. (opium poppy).

Cited by 8 publications

References 15 publications

Insights into opium poppy (Papaver spp.) genetic diversity from genotyping-by-sequencing analysis

Insights into opium poppy (Papaver spp.) genetic diversity from genotyping-by-sequencing analysis

Insights into opium poppy (Papaver spp.) genetic diversity from genotyping-by-sequencing analysis

Historical Insights into Some Contemporary Problems in Somatic Embryogenesis

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