Cytologic Evaluation of Otic Exudates

Chickering, William R.

doi:10.1016/s0195-5616(88)50080-3

Cited by 18 publications

(22 citation statements)

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“…Some authors have suggested <10 yeast cells per high-powered oil immersion field may be normal flora. 47,48 A subsequent semiquantitative analysis of potential otic pathogens suggests ‡5 yeast cells per high powered dry field is consistent with pathology in a dog. 49 However, it is recognized that a combination of cytology findings and clinical signs must correlate to warrant treatment of a clinically significant otitis.…”

Section: Inmentioning

confidence: 76%

“…Cytology may have relatively low sensitivity for yeast, 46 so baseline cytology may have been misleading in the case of the four dogs with negative cytology. Some authors have suggested <10 yeast cells per high‐powered oil immersion field may be normal flora 47,48 . A subsequent semiquantitative analysis of potential otic pathogens suggests ≥5 yeast cells per high powered dry field is consistent with pathology in a dog 49 .…”

Section: Discussionmentioning

confidence: 99%

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Safety and tolerability of 0.1% tacrolimus solution applied to the external ear canals of atopic beagle dogs without otitis

Kelley

Flynn-Lurie

House

et al. 2010

Veterinary Dermatology

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Tacrolimus is a nonsteroidal alternative to treat noninfectious otitis externa (OE) in people. This 21-day study investigated whether twice daily application (0.2 mL/dose) of sterile olive oil based 0.1% tacrolimus suspension in ears of atopic beagle dogs without OE was associated with adverse local reactions, development of OE, change in otic cytology, vestibular dysfunction, or hearing loss detected by brainstem auditory evoked response (BAER). The study was randomized, double-blinded, and placebo-controlled. Twenty-two dogs matched for age and sex were randomized to tacrolimus or vehicle control treatment groups. Two investigators independently evaluated dogs for signs of adverse effects including OE the first 4 days of treatment, then every 3 days. A logistic regression model was fit for each investigator's clinical scores (SAS, 9.2, 2008). Time (P = 0.0032) and group (P = 0.0167) were always significant for OE. Inter-observer reliability of clinical scores was strong, measured using Kappa coefficients and proportion of agreement. All nine exclusions (7/10 control- and 2/12 tacrolimus-treated dogs) were excluded for yeast OE. Inter-observer agreement to exclude was 100%. All dogs not excluded had normal BAER assessments before treatment, weekly during treatment, and after 21 days of treatment. None showed vestibular abnormalities at these times. Tacrolimus blood concentrations (Abbott IMx Tacrolimus II) were below detection limits (3 ng/mL) at baseline and after 21 days of treatment. Results suggest otic application of olive oil based tacrolimus suspension to canine ears with intact tympanic membranes is unlikely to result in hearing loss or vestibular dysfunction but yeast OE is a possible risk.

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Section: Inmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Safety and tolerability of 0.1% tacrolimus solution applied to the external ear canals of atopic beagle dogs without otitis

Kelley

Flynn-Lurie

House

et al. 2010

Veterinary Dermatology

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“…Também foi observada a presença e tipo de secreção otológica, classificadas como: amarelada, esverdeada, marrom ou purulenta (Chickering, 1988). A presença ou não de prurido ótico foi determinada de acordo com a percepção do proprietário.…”

Section: Methodsunclassified

Perfil clínico e microbiológico de cães com e sem otoacaríase

Souza

Scott

2015

Arq. Bras. Med. Vet. Zootec.

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RESUMOOs objetivos do estudo foram identificar a presença de microrganismos nos condutos auditivos dos cães através dos exames citológico e microbiológico, assim como avaliar a associação destes à otoacaríase e seus sinais clínicos. O diagnóstico da infestação por Otodectes cynotis foi realizado através de otoscopia bilateral e pela coleta de material e visualização do parasito sob microscópio esterioscópico, constituindo o exame parasitológico. Outras coletas de secreção otológica de cada orelha foram realizadas para confecção de lâminas para a citologia e para o isolamento microbiológico, sendo este último material coletado através de "swab" estéril. Dos 250 animais examinados, em 15 (6%) foi identificado o ácaro O. cynotis. Observou-se associação entre a presença do parasito e a ocorrência de otite clínica externa caracterizada especialmente por eritema auricular (P<0,001) e a produção excessiva de secreção otológica (P=0,0016), assim como a observação, pelos proprietários, de prurido ótico nos cães (P<0,001). Sugerese a possibilidade de essas associações serem efeito da infestação. Houve também a associação (P<0,01) entre a otoacaríase e a ocorrência de microrganismos nos dois condutos auditivos de cada animal, detectados através da citologia. No entanto, não houve associação (P=0,04 e p=0,07) entre a presença do ácaro O. cynotis e a ocorrência de microrganismos no exame microbiológico das orelhas direita e esquerda, respectivamente. Palavras-chave: cão, Otodectes cynotis, otite externa ABSTRACT The goals of this study were to identify microorganisms in dogs

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“…Swab cytology is an easy and rapid tool to assess bacterial or yeast infections of the external ear canal. 1 Cerumen is usually collected with a cotton swab or an ear loop directly from the external ear canal or from the tip of the otoscope cone and smeared on a glass microscope slide. 2 Some authors believe that waxy discharges should be heat-fixed with a flame before staining because this procedure helps to prevent the sample from being washed away during processing.…”

mentioning

confidence: 99%

“…[2][3][4][5] Smears on glass slides are then stained with modified Wright's stains, such as Diff-Quik (Baxter Diagnostic AG, Dubingen, Switzerland), or Hemacolor (Merck, Darmstadt, Germany) by dipping glass slides (5-10 seconds) in methanol, eosin (red reagent), and thiazine (blue reagent, counterstain), and rinsing them in water. [1][2][3][4][5] Cytologic examination of external ear canal specimens, processed as described above, usually reveals keratinocytes, Malassezia yeast organisms, bacteria, and neutrophils, helping determine the presence and type of inflammation and infectious agents in the ear. 1,[3][4][5] The usefulness of prior heat fixation is a matter of some debate 5 ; clinical dermatologists support its use, [2][3][4] but clinical pathologists do not think it is needed.…”

mentioning

confidence: 99%