Cytokinins differentially affect regeneration, plant growth and antioxidative enzymes activity in chive (Allium schoenoprasum L.)

Tubić, Ljiljana; Savić﻿﻿﻿, Jelena; Mitić, Nevena; Milojević, Jelena; Janošević, Dušica; Budimir, Snežana; Zdravković-Korać, Snežana

doi:10.1007/s11240-015-0869-1

Cited by 26 publications

(11 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Since using different levels of BA and NAA transformed the natural phenotype of the roots and considering the importance of being trueto-type in the in vitro cultures, some problems may arise in absorbing nutrients. Morphological disorders in the roots in our study might have been due to the presence of BA that quite often causes morphological and physiological disorders in plants (Tubić et al, 2016). Root disorders is more than just a morphological abnormality, since it affects the overall growth of the plant (Singh, 2018).…”

Section: Discussionmentioning

confidence: 74%

“…El Tahchy et al (2011) employed different combinations and concentrations of 2, 4-D, BA, sucrose, and activated charcoal for induction of callus from leaves. Among the commercially available cytokinins, BA has been used more frequently, regarding to its low price and high efficacy in the promotion of in vitro regeneration in various plant species (Tubić et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

In vitro regeneration of bulblet using two and four bulb-scales explants of summer snowflake (Leucojum aestivum L.)

Abedinimazraeh

Kalatehjari

2021

Ornam. Hortic.

View full text Add to dashboard Cite

Leucojum aestivum is a valuable and endangered plant species with bulb scales best suited as explants in micropropagation. In the current study, its micropropagation was investigated by using two different explants and various concentrations and combinations of plant growth regulators (PGRs). Bulbs were first disinfected with benomyl® for 5 hours. After meeting the chilling requirements, two-scale and four-scale explants were provided for direct and indirect organogenesis. Explants were exposed to hot water, 70% ethanol and 2.5% sodium hypochlorite for further disinfestation. Four-scale explants were treated with different concentrations and combinations of naphthaleneacetic acid (NAA), 6-benzyladenine (BA), and kinetin (Kin) for bulblet regeneration. For callogenesis, 0.5 mg L-1 of BA combined with 1, 2, 3, 4, 5 or 6 mg L-1 of 2,4-dichlorophenoxyacetic acid (2,4-D) were applied. Regarding two-scale explants, different combinations and concentrations of BA, Indole-3-butyric acid (IBA) and NAA were used for bulblet induction, and various combinations of Indoleacetic acid (IAA), NAA, 2, 4-D and BA were used for callus induction. None of the two-scale explants responded to the bulblet regeneration and callus induction media. Unlike, four-scale explants regenerated bulblets and roots in the control medium and MS media enriched with different PGRs. Callus was generated on MS medium supplemented with 2,4-D and BA, and indirect regeneration was observed in some cases. On the control medium, the regenerated roots had a natural form, but in PGRs-rich media, they were deformed. Regarding the regeneration percentage, bulblet number and length and root length, no significant differences were found between the control and the best PGR-treatment in each case. Therefore, it seems logical suggesting not to use PGRs, which will considerably reduce the costs at large-scale production.

show abstract

Section: Discussionmentioning

confidence: 74%

Section: Introductionmentioning

confidence: 99%

In vitro regeneration of bulblet using two and four bulb-scales explants of summer snowflake (Leucojum aestivum L.)

Abedinimazraeh

Kalatehjari

2021

Ornam. Hortic.

View full text Add to dashboard Cite

show abstract

“…In addition to the plant species factor, these differences could be related to the POX type, preparation protocol, and the substrates used in activity tests. Indeed, it has been shown that the use of certain substrates in the POXs tests could produce latencies, which result in an underestimation of POXs activities [ 63 ].…”

Section: Discussionmentioning

confidence: 99%

Peroxidase Enzyme Fractions as Markers of Somatic Embryogenesis Capacities in Olive (Olea europaea L.)

Oulbi

Kohaich

Baaziz

et al. 2021

Plants

View full text Add to dashboard Cite

As part of the search for biochemical markers of somatic embryogenesis in tissue cultures of olive (Olea europaea L.), peroxidases (POXs) in both the soluble and ionically wall-bound fractions were studied in two reputed olive cultivars (cvs.): “Picholine Marocaine” and “Dahbia”. In order to carry out embryogenesis induction, proximal cotyledons were cultured in modified olive medium (OMc) supplemented with 25 μM indole-3-butylic acid (IBA) and 2.5 μM 2-isopentenyladenine (2iP), while distal leaf fragments (somatic explants) were cultured in OMc supplemented with 4.56 µM zeatin riboside (ZR) and 10.25 µM 1-naphthaleneacetic acid (NAA). Regarding embryogenic potentials, the zygotic explants (cv. Picholine Marocaine: 43.39%; cv. Dahbia: 53.41%) were more regenerative than the somatic explants (cv. Picholine Marocaine: 13.05%; cv. Dahbia: 19.51%). The enzyme assay showed a higher POX activity in embryogenic calluses (ECs) than in nonembryogenic calluses (NECs) for the zygotic explants in both studied cultivars. When expressed as units per milligram of proteins (U mg−1 proteins), the highest total POXs activities (soluble POXs + ionically wall-bound POXs) were found in the ECs derived from the zygotic explants; for cv. Dahbia, 65% of the enzyme activities came from the ionically wall-bound fractions. Polyacrylamide gel electrophoresis showed that the ECs of the highly active cv. Dahbia were characterized by highly active isoperoxidases that were revealed in four migration zones, particularly a doublet in the A4 zone (Rf 0.70–0.73) present in the ionically wall-bound POXs. The fast-moving anodic POXs of the ionically wall-bound fractions could be adopted as an early electrophoretic test to determine the embryogenesis capacities in olive tissue culture materials. As biochemical markers, the POX enzyme and its profile in fractions, i.e., as soluble POXs and ionically wall-bound POXs, can offer a valuable tool for improving the tissue culture of olive via somatic embryogenesis.

show abstract

“…У науковій літературі достатньо інформації про застосування нативних та синтетичних рістстимуляторів із метою активізації продукційного процесу шляхом морфометричних змін у зерново-круп᾽яних (Javid et al, 2011;Muhammad and Muhammad, 2013;Luo et al, 2016;Zhao et al, 2017), зернобобових (Xing et al, 2016), олійних (Rogach, 2009;Khodanitska and Kuryata, 2011;Fu et al, 2014;Froschle et al, 2017), овочевих (Xiaotao et al, 2013Tubiс et al, 2016;Alexopoulos et al, 2017), технічних (Khodanitska and Kuryata, 2011;Mohammad and Mohammad, 2013;Rai et al, 2017), фруктових (Ahmed et al, 2012;Cruz-Castilloa et al, 2014;Li et al, 2016), лікарських і декоративних (Gouveia et al, 2012;Aremu et al, 2017;Madzikane-Mlungwana et al, 2017) культурах. Стимулятори росту також підвищують стійкість сільськогосподарських культур до несприятливих абіотичних та біотичних факторів середовища завдяки змінам у гормональному статусі та активізації антиоксидантних систем рослинного організму (Javid et al, 2011;Muhammad and Muhammad, 2013;Piotrowska-Niczyporuk et al, 2014;Tubiс et al, 2016;Xing et al, 2016).…”

Section: вступunclassified

“…У літературних джерелах міститься інформація щодо застосування цитокінінових препаратів в аграрній практиці. Похідні фенілсечовинитидіазурон і N-(2-хлор-4-піридил)-N-фенілсечовина збільшували кількість бруньок у цибулі (Tubiс et al, 2016). Найпоширеніший представник іншої групи цитокінінів -N-оксид заміщених піридинуце 2,6-диметилпіридин-1-оксид.…”

Section: вступunclassified

Influence of growth stimulants on photosynthetic apparatus, morphogenesis and production process of eggplant (Solanum melongena)

Rohach

2017

Biosys. divers.

View full text Add to dashboard Cite

Artificial regulation of the growth and development of cultivated plants aiming at an increase of biological productivity and improvement of the quality of agricultural products is a significant objective of modern phytophysiology. The use of natural and synthetic growth stimulators is quite effective. The present paper discusses the issue of the influence of gibberellin acid (GA3), 1-naphthylacetic acid (1-NAA) and 6-benzylaminopurine (6-BAP) on the morphogenesis and productivity of aubergine plants of Diamond variety. Field experiments were conducted in 2013–2015. The plants were treated with such growth stimulators as 1-NAA, GA3 and 6-BAP at the concentration of 0.005% using a portable sprayer. The phytometric indices were fixed every 10 days, the mesostructure was checked in the leaves of the middle layer in the phase of fruit formation, the content of the amount of chlorophylls was determined in the fresh material by spectrophotometric method, the content of different forms of carbohydrates and nitrogen in the plant organs was estimated by biochemical methods in fixed dry material. It was found that GA3 increased plant height on average by 16.3%. 1-NAA and 6-BAP practically did not change linear plant sizes. The germicides increased the number of leaves per plant (10.8–30.8%), the mass of their raw matter (19.9–47.5%) and the area of the leaf surface (17.5–42.5%). The most significant impact on these indicators was made by GA3. All the germicides increased the number of leaves per plant (14.3–20.9%). 6-BAP increased the thickness of the leaf blades by 6.3% due to the growth of chlorenchyma, and GA3 reduced it by 9.2%. Under the influence of 6-BAP and 1-NAA, the thickness of chlorenchyma was increased by 7.0% and 5.9% respectively.The upper and lower epidermis became thinner or did not change under the effects of these germicides. Under the plant treatment with 1-NAA and 6-BAP, the size of columnar parenchyma cells increased by 25.6% and 19.6% and the size of the spongiform parenchyma cells increased by 8.4–76.7%. Under the action of GA3, the cell volume of the columnar parenchyma did not change significantly, and the size of the spongy cells increased. This paper reveals that all growth stimulants reduced the number of epidermis cells (6.6–7.4%). Under the action of 1-NAA and 6-BAP, there was a decrease in the number of stomata per 1 mm2 of the abacus leaf surface, respectively,by 6.5% and 21.2%. Instead, after the use of GA3, an increase in the number of stomata was observed by 21.8%. Such germicides as 1-NAA and GA3 reduced the area of respiratory area by 11.7% and 21.4%, while the 6-BAP increased its area by 10.4%. The results of the research show that 6-BAP increased the content of the sum of chlorophylls a + b in leaves by 13.3%. Under the action of 1-NAA, this indicator had only a tendency to increase (6.7%), but under the influence of GA3 it decreased. Moreover, the processing with germicides significantly increased leaf and chlorophyll indices. All three growth stimulants have increased the mass of dry matter of plants and the net productivity of photosynthesis. The data demonstrate that growth stimulants have contributed to the accumulation of various forms of carbohydrates in the roots and fruits. In the stems and leaves there was a tendency to decrease the content of sugars and starch. The germicides significantly reduced the content of all forms of nitrogen in the roots, stems and fruits, and increased the content of protein nitrogen in the leaves. Under the action of GA3 and 6-BAP, the number of fruits per plant increased by 19.3% and 16.1%, respectively. All growth stimulants have significantly increased the average weight of the individual fetus (7.4–10.3%). As a result, the weight of the fruits from one plant after treatment with 1-NAA, GA3 and 6-BAP increased by 11.0%, 28.0% and 29.4%, respectively. There are grounds to think that growth stimulants, influencing anatomical, morphological, physiological and biochemical characteristics of aubergines, changed the nature of the donor-acceptor relationships in the plant, which intensified the production process and optimized its productivity.

show abstract

Cytokinins differentially affect regeneration, plant growth and antioxidative enzymes activity in chive (Allium schoenoprasum L.)

Cited by 26 publications

References 44 publications

In vitro regeneration of bulblet using two and four bulb-scales explants of summer snowflake (Leucojum aestivum L.)

In vitro regeneration of bulblet using two and four bulb-scales explants of summer snowflake (Leucojum aestivum L.)

Peroxidase Enzyme Fractions as Markers of Somatic Embryogenesis Capacities in Olive (Olea europaea L.)

Influence of growth stimulants on photosynthetic apparatus, morphogenesis and production process of eggplant (Solanum melongena)

Contact Info

Product

Resources

About