Cytokinin perception in potato: new features of canonical players

Lomin, Sergey N.; Myakushina, Yulia A.; Kolachevskaya, Oksana O.; Getman, Irina A.; Arkhipov, Dmitry V.; Savelieva, Ekaterina M.; Osolodkin, Dmitry I.; Romanov, Georgy A.

doi:10.1093/jxb/ery199

Cited by 28 publications

(56 citation statements)

References 78 publications

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“…CisZ has low cytokinin activity, its affinity for potato CK-receptors is approx. 40-50-fold lower than the affinity of tZ or iP 18 . CisZ-type CKs often occur at a relatively high concentration in plant organs with limited growth (storage organs) 19,20 and can attenuate the cytokinin signaling wherein by competing with active cytokinins.…”

mentioning

confidence: 85%

Core features of the hormonal status in in vitro grown potato plants

Kolachevskaya¹,

Сергеева²,

Getman³

et al. 2018

Plant Signaling & Behavior

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Some time ago, potato transformants expressing Agrobacterium-derived auxin synthesis gene tms1 were generated. These tms1-transgenic plants, showing enhanced productivity, were studied for their hormonal status, turnover and responses in comparison with control plants. For this purpose, contents of phytohormones belonging to six different classes (auxins, cytokinins, gibberellins, abscisic, jasmonic and salicylic acids) were determined by a sensitive UPLC-MS/MS method in tubers and shoots of in vitro grown plants. To date, this study represents the most comprehensive analysis of the potato hormonal system. On the basis of obtained results, several new generalizations concerning potato hormonal status were drawn. Overall, these data can serve as a framework for forthcoming integrative studies of the hormonal system in potato plants.

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mentioning

confidence: 85%

Core features of the hormonal status in in vitro grown potato plants

Kolachevskaya¹,

Сергеева²,

Getman³

et al. 2018

Plant Signaling & Behavior

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“…The effect of each CK ligand depends not only on its structure and concentration, however it also may be species-specific. Various CKs show modified ligand affinity for their receptors, e.g., in A. thaliana , Brasica napus , maize and potato [22,149,150,151]. The diverse effects of particular CKs regarding physiological responses to exogenous application have also been documented.…”

Section: Cks and Photosynthesismentioning

confidence: 99%

Role of Cytokinins in Senescence, Antioxidant Defence and Photosynthesis

Hönig

Plíhalová

Husičková

et al. 2018

IJMS

153

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Cytokinins modulate a number of important developmental processes, including the last phase of leaf development, known as senescence, which is associated with chlorophyll breakdown, photosynthetic apparatus disintegration and oxidative damage. There is ample evidence that cytokinins can slow down all these senescence-accompanying changes. Here, we review relationships between the various mechanisms of action of these regulatory molecules. We highlight their connection to photosynthesis, the pivotal process that generates assimilates, however may also lead to oxidative damage. Thus, we also focus on cytokinin induction of protective responses against oxidative damage. Activation of antioxidative enzymes in senescing tissues is described as well as changes in the levels of naturally occurring antioxidative compounds, such as phenolic acids and flavonoids, in plant explants. The main goal of this review is to show how the biological activities of cytokinins may be related to their chemical structure. New links between molecular aspects of natural cytokinins and their synthetic derivatives with antisenescent properties are described. Structural motifs in cytokinin molecules that may explain why these molecules play such a significant regulatory role are outlined.

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“…The cytosolic part of the receptor includes the catalytic module, which consists of HK-dimerization (HisKA or DHpD) and H-ATPase (CAD) domains [6,7]. In addition, the receptor possesses the receiver-like (RLD or REC-like) and receiver (RD or REC) domains (considered together as a receiver module), which are located at the C-terminus of the HK protein [8,9].…”

Section: Introductionmentioning

confidence: 99%

Modeling of Protein–Protein Interactions in Cytokinin Signal Transduction

Arkhipov

Lomin

Myakushina

et al. 2019

IJMS

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The signaling of cytokinins (CKs), classical plant hormones, is based on the interaction of proteins that constitute the multistep phosphorelay system (MSP): catalytic receptors—sensor histidine kinases (HKs), phosphotransmitters (HPts), and transcription factors—response regulators (RRs). Any CK receptor was shown to interact in vivo with any of the studied HPts and vice versa. In addition, both of these proteins tend to form a homodimer or a heterodimeric complex with protein-paralog. Our study was aimed at explaining by molecular modeling the observed features of in planta protein–protein interactions, accompanying CK signaling. For this purpose, models of CK-signaling proteins’ structure from Arabidopsis and potato were built. The modeled interaction interfaces were formed by rather conserved areas of protein surfaces, complementary in hydrophobicity and electrostatic potential. Hot spots amino acids, determining specificity and strength of the interaction, were identified. Virtual phosphorylation of conserved Asp or His residues affected this complementation, increasing (Asp-P in HK) or decreasing (His-P in HPt) the affinity of interacting proteins. The HK–HPt and HPt–HPt interfaces overlapped, sharing some of the hot spots. MSP proteins from Arabidopsis and potato exhibited similar properties. The structural features of the modeled protein complexes were consistent with the experimental data.

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Cytokinin perception in potato: new features of canonical players

Abstract: Potato genes for putative cytokinin receptors were cloned and expressed. The proteins displayed properties of cytokinin receptors, and their expression in planta was organ specific and sensitive to growth conditions and sucrose content.

Cited by 28 publications

References 78 publications

Core features of the hormonal status in in vitro grown potato plants

Core features of the hormonal status in in vitro grown potato plants

Role of Cytokinins in Senescence, Antioxidant Defence and Photosynthesis

Modeling of Protein–Protein Interactions in Cytokinin Signal Transduction

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