Core features of the hormonal status in in vitro grown potato plants

Abstract

Some time ago, potato transformants expressing Agrobacterium-derived auxin synthesis gene tms1 were generated. These tms1-transgenic plants, showing enhanced productivity, were studied for their hormonal status, turnover and responses in comparison with control plants. For this purpose, contents of phytohormones belonging to six different classes (auxins, cytokinins, gibberellins, abscisic, jasmonic and salicylic acids) were determined by a sensitive UPLC-MS/MS method in tubers and shoots of in vitro grown plants. To date, this study represents the most comprehensive analysis of the potato hormonal system. On the basis of obtained results, several new generalizations concerning potato hormonal status were drawn. Overall, these data can serve as a framework for forthcoming integrative studies of the hormonal system in potato plants.

Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics

“…Our results for endogenous IAA content (82.0 pmol/gFW in shoots and 62.3 pmol/gFW in roots) were similar to others reported for in vitro grown potato 27,31,33 but considerably lower than those found in greenhouse-grown plants 24 . It has been reported that the IAA content of in vitro grown shoots of the potato cultivar Miranda amounted to 81 pmol/gFW 27 .…”

“…The most abundant CK nucleobase in the shoots in our study was iP, while in the roots it was tZ. iP was previously shown to be the most abundant CK nucleobase in potato shoots 32,33 . It has been shown that the biological activity of iP is important for physiological processes in plant shoots 43 .…”

“…There is growing research interest for CK physiology in potato (Solanum tuberosum L.) due to numerous evidence for the involvement of CKs in the commercially important process of tuberisation [18][19][20][21][22][23] . A number of reports containing measurements of phytohormones in various tissues of potato plants grown in various conditions (in vitro, greenhouse) have been published so far 22,[24][25][26][27][28][29][30][31][32][33] . Data on amounts of particular molecular forms of isoprenoid CKs, as well as the auxin indole-3-acetic acid (IAA), and abscisic acid (ABA), measured in control plants in these studies, are summarised in Table 1.…”

“…Among the publications listed in Table 1, only few include reports on endogenous levels of certain CK conjugates 25 or cZ-type CKs 32,33 in potato. The first report on the whole CK profile of potato shoots and roots (cv.…”

Cytokinin perception in potato: New features of canonic players

“…All StHKs demonstrated high affinity for trans -zeatin, with similar K d at the nanomolar level (Table 3). The determined K d values were close to the values of analogous constants for CK receptors of other species (Lomin et al ., 2012, 2015, Kuderová et al ., 2015) and were well correlated with concentrations of active CKs in planta (Hirose et al ., 2008) including potato (Kolachevskaya et al ., 2017, 2018).…”