CYTOKINETIC ANALYSIS of SLOWLY PROLIFERATING BONE MARROW CELLS DURING RECOVERY FROM RADIATION INJURY

Haas, Rainer; Bohne, F.; Fliedner, Theodor M.

doi:10.1111/j.1365-2184.1971.tb01515.x

Cited by 16 publications

(12 citation statements)

References 17 publications

(7 reference statements)

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“…Therefore, the interpretation would again be that only few, if any, of these cells did react to antigen stimula tion. Similar findings could be demonstrated under regeneratory demand [6,7]. However, the reticular cells and histiocytes of the spleen showed a decrease of their labelling index without a marked diminuation of the mean grain count.…”

Section: Discussionsupporting

confidence: 74%

“…In the spleen of mice only 0.10-0.20 immuno competent cells for a specific antigen could be detected per 10° cells [1,2,8], Therefore, the conclusion would be that under immune stimulation, the mass of long-living lymphocytes does not react in the sense of a trans formation or proliferation. In contrast to that behaviour, as could be shown in previous work, the mass of bone-marrow lymphocytes did react under regeneratory stimulus [6,7].…”

Section: Discussioncontrasting

confidence: 47%

“…mation of such resting bone-marrow lymphocytes during regeneration of an aplastic bone marrow were shown [6,7].…”

mentioning

confidence: 99%

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Cytokinetic Studies on Slowly-Renewing Bone Marrow and Spleen Lymphocytes in Rats During a Primary and a Secondary Immune Response

Rj¹,

Kd²,

Trepel³

et al. 1972

Acta Haematol

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In growing rats, slowly-turning-over cells were labelled by application of ³H-TdR for 1–6 Four weeks after the last ³H-TdR injection, all short-living cells had lost their label, while long-living small Spleen lymphocytes lymphocytes and cells of Immune responsesthe bone marrow and the spleen had retained it. After Lymphocytesprimary and secondary exposure to antigens (human aγ-globulin plus complete Freund’s adjuvant), labeling of bonemarrow lymphocytes remained stable, where- as the labelling index of spleen lymphocytes markedly decreased. It is, therefore, concluded that at least the majority of long-living small lymphocytes in the bone marrow, in contrast to long-living small lymphocytes of the spleen, do not participate in a primary and secondary immune reaction against these antigens.

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Section: Discussionsupporting

confidence: 74%

Section: Discussioncontrasting

confidence: 47%

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Cytokinetic Studies on Slowly-Renewing Bone Marrow and Spleen Lymphocytes in Rats During a Primary and a Secondary Immune Response

Rj¹,

Kd²,

Trepel³

et al. 1972

Acta Haematol

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“…The term endothelial cells is meant for both the sinusoidal lining cells and the true vascular endothelial cells (for detailed description see else where [9,11]). It was shown that after administration of X-irradiation or cytotoxic agents [1,10,13] during the regeneratory process the frac tion of small mononuclear cells called 'resting bone marrow lymphocytes' could be stimulated to proliferate. However, there was no evidence for a stimulation of reticular or endothelial cells demonstrable in these investi gations.…”

mentioning

confidence: 99%

Cytokinetics of Bone Marrow Stroma Cells after Stimulation by Partial Depletion of the Medullary Cavity

Meyer‐Hamme¹,

Haas²,

Fliedner³

1971

Acta Haematol

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The continuous administration of tridated thymidine (³H-TdR) into pregnant rats resulted in a complete labelling of cell nuclei in the offspring. Further injections with ³H-TdR for 6 weeks maintained the 100-percent labelling. Six weeks after the last ³H-TdR application, only cytokinetically resting cells were selectively labelled in the bone marrow of these animals. These comprised reticular cells, endothelial cells and 5% of the bone marrow lymphocyte population. In these animals a standardised amount of the marrow of both the femora was removed by mechanical means. In the undepleted part of the femur a stimulation of formerly cytokinetically resting cells could be observed. There was no indication of stimulation in distant bone marrow parts. In an additional experiment in which a single injection of ³H-TdR was given at various intervals after depletion, the above findings were supported by a high uptake of ³H-TdR in the stroma cells of the bone marrow. The findings indicate that repopulation of bone marrow stroma cells is a necessary process for a restoration of haemopoiesis.

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“…It has been shown that these cells have a short life in the bone marrow (Elves 1972). There are few long-lived lymphocytes in the rodent bone marrow (Haas et al 1971). Morphologically there are little differences among the bone marrow lymphocytes in rodents as well as in the human.…”

Section: Commentmentioning

confidence: 93%

The in vitro development of immunoglobulin producing cells from the human bone marrow null lymphocyte.

Kuroyanagi

Kura²

1981

Tohoku J. Exp. Med.

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The distribution of human bone marrow lymphocyte population was studied on twenty normal adults. The distribution of bone marrow lymphocytes were as follows : null lymphocytes 79.4%, E-RFC 8.9%, Thy+ lymphocytes 0.4%, Ty 0.9%, Tµ 1.4%, SmIg+ cells 7.8%, EAC-RFC 11.7%, EA-RFC 6.2%, and K cells 2.7%. Bone marrow null lymphocytes were intermediate in their size between small lymphocytes and lymphoblasts. The presence of a lot of short microvilli on null lymphocytes was demonstrated by scanning electron microscopic examinations. The bone marrow null lymphocytes had no cytoplasmic Ig. When the bone marrow null lymphocytes were mixedlymphocyte-cultured with allogeneic T lymphocytes and then stimulated with PWM, there developed Ig producing cells. Differences between pre-B cells and bone marrow null lymphocytes were discussed.human bone marrow lymphocyte population; bone marrow null lymphocyte; development of Ig producing cells; pre-B cellThe nature of the human bone marrow lymphocyte population remains still to be clarified. In mice the distribution of lymphocytes bearing mouse specific B lymphocyte antigen (MBLA), surface membrane Immunoglobulin (SmIg), complement receptor and Fc receptor is 25 N 45%, 5 N 15%, 5 N 10% and 10 N 15%, respectively (Greaves et al. 1973). A small portion of mice bone marrow lymphocytes is known to be T lymphocytes. According to Douglas (1976), more than 75% of human bone marrow lymphocytes are B lymphocytes and less than 25% are T lymphocytes.However, little is known about the exact distribution of the human bone marrow lymphocytes population. The population of normal human bone marrow lymphocytes was determined and the morphology of bone marrow null lymphocytes was studied by transmission and scanning electron microscopy.The in vitro development of Ig producing cells from the human bone marrow lymphocytes under the regulation of T lymphocytes by the PWM stimulation was also examined.

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CYTOKINETIC ANALYSIS of SLOWLY PROLIFERATING BONE MARROW CELLS DURING RECOVERY FROM RADIATION INJURY

Cited by 16 publications

References 17 publications

Cytokinetic Studies on Slowly-Renewing Bone Marrow and Spleen Lymphocytes in Rats During a Primary and a Secondary Immune Response

Cytokinetic Studies on Slowly-Renewing Bone Marrow and Spleen Lymphocytes in Rats During a Primary and a Secondary Immune Response

Cytokinetics of Bone Marrow Stroma Cells after Stimulation by Partial Depletion of the Medullary Cavity

The in vitro development of immunoglobulin producing cells from the human bone marrow null lymphocyte.

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