CYTOKINESIS BLOCK MICRONUCLEUS ASSAY IN HUMAN LYMPHOCYTES AFTER EXPOSURE TO Ru(III) THIOSEMICARBAZONE COMPLEXES IN VITRO

Ljubijankić, Nevzeta; Stanković, Miroslava; Tešević, Vele; Grgurić-Šipka, Sanja; Jadranin, Milka; Bеgić, Sаbinа; Šabanović, E.

doi:10.31788/rjc.2018.1123004

Cited by 8 publications

(12 citation statements)

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“…Schiff bases as a compound with azomethine linkage (-C=N-) are versatile and very popular ligands for metal coordination 1,2 . Since Schiff bases show diverse biological activity, a significant number of metal complexes with various ligands have been synthesized and their activities against different targets have been investigated [3][4][5][6][7][8] . Antibacterial, antifungal, antiviral, antioxidant and cytotoxic activities have been reported 1,[9][10][11][12] .…”

Section: Introductionmentioning

confidence: 99%

“…Ruthenium(III) complexes are of immense interest regarding their kinetic inertness 13 . Some ruthenium(III) complexes containing ON or ONS donor atoms were synthesized in our laboratory regarding interaction with DNA or genotoxic activity have been previously reported 4,5,14 . Ru(III) complexes containing indazole, imidazolium trans-[tetrachlorobis(1H-indazole)ruthenate(III)] (KP1019) and sodium trans-[tetrachlorobis(1H-indazole)ruthenate(III)] (NKP-1339) have proofed an extraordinary antiproliferative properties and entered clinical trials 15 .…”

Section: Introductionmentioning

confidence: 99%

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Novel Complexes of Ruthenium(iii) With Schiff Bases and Indazole – Synthesis and Characterization

Begić¹,

Ljubijankić²,

Gojak-Salimović³

et al. 2019

RJC

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Two novel cationic Ru(III) complex compounds with Schiff bases, N-phenyl-5-X-salicylideneimine (where X = H or Br) and indazole have been synthesized and characterized by various spectral and electrochemical analysis. The existence of molecular ions M + at m/z 730.1652 and 887.9818 corresponding to [C40H32N6O2Ru] + and [C40H30Br2N6O2Ru] + , respectively, was obtained by mass spectra analysis. The coordination of the ligands in the Ru(III) octahedral environment occurs through imine nitrogen, deprotonated phenolic oxygen atom from the Schiff bases as well as via the free electronic pair on the nitrogen atom in indazole. Furthermore, the effect of the cationic Ru(III) complexes on the Briggs-Rauscher oscillating reaction was preliminary studied and monitored potentiometrically.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Novel Complexes of Ruthenium(iii) With Schiff Bases and Indazole – Synthesis and Characterization

Begić¹,

Ljubijankić²,

Gojak-Salimović³

et al. 2019

RJC

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“…15,11 The biological activity of the potential drug in the pre-clinical investigations should be comprehensively examined with the aim to obtain as much information as possible about its therapeutic and side effects in the living organism. 17,18 In the last decade, there is a remarkable increase of bacterial pathogens that present multidrug resistance than currently available antimicrobial agents. Therefore, the development of new antimicrobial strategies and antibacterial agents is an active area of research.…”

Section: Introductionmentioning

confidence: 99%

“…23,24 In our previous paper, we studied in vitro effects of the Ru(III) thiosemicarbazone complexes on human lymphocytes using the CBMN assay. 18 The CBMN assay in peripheral human lymphocytes is well established cytogenetic technique and is developed to score micronuclei (MN) in different nucleated cell types in vitro or in vivo. 25,26 The analysis of micronuclei (MN) in cultured lymphocytes is frequently used as a preferred method to biomonitor human exposure to genotoxic agents.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial and Genotoxic Activity of Novel Ruthenium(iii) Complex With N-Phenyl-5-Nitrosalicylideneimine

Ljubijankić¹,

Begić²,

Ljubović-Dedeić³

et al. 2018

RJC

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In this study, novel hexa coordinated ruthenium(III) complex of the type Na[RuCl2L2)] (where L = monobasic bidentate Schiff base derived from the condensation of 5-nitrosalicyladehyde with aniline) has been synthesized and characterized by electrospray ionization time-of-flight mass spectrometry, infrared spectroscopy and ultraviolet/visible spectrophotometry. Schiff base N-phenyl-5-nitrosalicylideneimine is coordinated to the ruthenium via imine nitrogen and phenolic oxygen. Mass spectra showed molecular ion (M -) at m/z 653.9641 which corresponds to [C26H18Cl2N4O6Ru] -. The in vitro antimicrobial properties of the Schiff base and the complex were tested by micro-dilution technique and agar plate assay for determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The compounds showed a higher antibacterial activity against tested Gram-positive bacteria (Staphylococcus aureus ATCC 33591 and ATCC 29213), whereas against the Gram-negative bacteria (Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 700603) were ineffective. The genotoxic effects of Ru(III) complex were investigated using the Cytokinesis Block Micronucleus (CBMN) assay in human lymphocytes cultures. The cell culture treated with the complex at a concentration of 3.7 µg/mL exhibit the most prominent effect of decreasing the frequency of micronucleus for 44%, while at the concentrations of 1.5 and 7.4 µg/mL effect is slightly lower (40%), compared to the control cell culture.

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“…Various compounds are introduced into the body by food or drugs, which may potentially interact with metals and thereby affecting their biological effect (Cipurković et al, 2017). Metal complexes stay a significant resource for creating chemical diversity in the fields of biological, pharmaceutical and medicinal chemistry as antitumor and antimicrobial agents (Ljubijankić et al, 2018). Analysis of the interaction of biological M(II) ions with O, N and Sdonor atoms of ligands, often used in the treatment of a wide spectrum of diseases, is important for pharmacokinetics, monitoring distribution, excretion, drug efficacy and monitoring adverse effects (Cipurković et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Synthesis, spectral characterization and antimicrobial activity of some M(II) complexes with Ciprofloxacin

Horozić

Cipurković

Ademović

et al. 2018

J. eng. process. manag.

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Ciprofloxacin, CFL is a drug that belongs to the second generation of fluoroquinolone antibiotics with a wide range of effects on Gram-positive and Gram-negative bacteria. The aim of this work was to investigate the interaction of CFL as ligand with divalent biological cations (Mn2+, Ni2+ and Co2+) in approximate physiological conditions. Synthesized complexes were characterized using FTIR and stereo-microscopy. Antimicrobial screening was performed on bacterial strains of Escherichia coli, Salmonella Enteritidis, Enterococcus faecalis and Staphylococcus aureus. The results of FTIR spectroscopy showed that the M(II) complexes with CFL were formed through the oxygen donors of the carboxyl and carbonyl group of the ligand. Stereo-microscopic characterization revealed the difference in color and size of crystals of the ligand and metal complexes. Antimicrobial screening has shown that CFL and complexes have almost similar antimicrobial activity against investigated bacterial strains.

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CYTOKINESIS BLOCK MICRONUCLEUS ASSAY IN HUMAN LYMPHOCYTES AFTER EXPOSURE TO Ru(III) THIOSEMICARBAZONE COMPLEXES IN VITRO

Cited by 8 publications

References 0 publications

Novel Complexes of Ruthenium(iii) With Schiff Bases and Indazole – Synthesis and Characterization

Novel Complexes of Ruthenium(iii) With Schiff Bases and Indazole – Synthesis and Characterization

Antimicrobial and Genotoxic Activity of Novel Ruthenium(iii) Complex With N-Phenyl-5-Nitrosalicylideneimine

Synthesis, spectral characterization and antimicrobial activity of some M(II) complexes with Ciprofloxacin

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