Cytokines in the modulation of eosinophilia

Faccioli, Lúcia Helena; Vargaftig, B. Boris; Medeiros, Alexandra I.; Malheiros, Adriana

doi:10.1590/s0074-02761997000800015

Cited by 11 publications

(10 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Both our own findings and literature data seem to indicate that parasitic invasions induce immune and inflammatory response, with the involvement of blood platelets and release of IFN-γ , IL-8, TNF-α and IL-5 [25,38].…”

Section: Studies Of Other Authors Have Shown That In Gsupporting

confidence: 74%

IFN-gamma, IL-5, IL-6 and IgE in patients infected with Giardia intestinalis.

Matowicka-Karna

Dymicka-Piekarska²,

Kemona³

2009

Folia Histochem Cytobiol.

View full text Add to dashboard Cite

Abstract:The immune system, its cellular and humoral response, is engaged by the host organism to fight against parasitic infections. The study group consisted of 90 patients (58 women and 32 men), aged 18-72 years, infected with G. intestinalis. The diagnosis was established based on laboratory investigations (stool examination, choloscopy, GSA-65). Blood for analysis was collected before (G1), and 2 weeks (G2) and 2 months (G3) after antiparasitic treatment. Control group consisted of 40 healthy subjects (22 women and 18 men), aged 20-45 years. The concentrations of IgE were assayed using a set of VIDAS (bioMerieux) and the concentrations of IL-5, IL-6, IFN-γ were determined using a set of Quantikine human (R&D Systems). It was revealed that in giardiosis the concentrations of IgE and IL-5 in blood serum were twice as high, the concentration of IL-6 was two and a half times higher and the concentration of IFN-γ was almost four times higher as compared to healthy controls.

show abstract

Section: Studies Of Other Authors Have Shown That In Gsupporting

confidence: 74%

IFN-gamma, IL-5, IL-6 and IgE in patients infected with Giardia intestinalis.

Matowicka-Karna

Dymicka-Piekarska²,

Kemona³

2009

Folia Histochem Cytobiol.

View full text Add to dashboard Cite

show abstract

“…to infect host cells and modulated immune response for surviving within the host. It was reported that the IL-5 cytokine stimulates eosinophil degranulation, production of reactive oxygen species, and exerting a chemotactic effect on these cells [39]. Nickdel et al [40] discussed that T. gondii infection cause's eosinophilia and increased the level of IL-5, with pathological changes in the small intestine, with a simultaneous reduction in IL-12 and IFN-γ.…”

Section: Discussionmentioning

confidence: 99%

Detection of Humoral and Cellular Immune Responses in Buffaloes Naturally Infected with Sarcocystosis with Risk Factor Assessment

2019

View full text Add to dashboard Cite

Sarcocystosis is a silent, parasitic disease which affects various animal species and causes significant economic losses. It is caused by a number of different intracellular Sarcocystis spp. This study was aimed to detect the host humoral and cellular immune response due to natural infection. Adding to the determination of the infection rate in Monufia Governorate, Egypt. A total number of 127 Egyptian buffaloes (Bubalus bubalis); 30 males and 97 females between 2-11 years of age were examined during 2018. An infection rate of 74% (94/127) was detected by macroscopic examination. The old age females were found to be at a high risk of 90.7% (88/97) in comparison with the young males 20% (6/30). Immunologically, the cellular and humoral immune response was determined using ELISA. A marked down-regulation of the proinflammatory Th-1 cytokine (IFN-γ) and up-regulation of the anti-inflammatory Th-2 cytokine (IL-5) adding to a high level of IgG and IgE were detected in the infected animals compared to the non infected ones. The local cellular immune response in the infected tissues was characterized by an accumulation of mixed inflammatory cells, granuloma formation, eosinophilic infiltration, muscular edema, and necrotic degeneration. In conclusion, the Sarcocystis infection rate in the naturally infected buffaloes in Monufia Governorate was high. This is the first study to provide a fundamental insight into the immune profile in buffaloes infected with Sarcocystis spp. So, it will provide valuable insights to develop novel effective vaccines in future studies. Moreover, sensitive and specific tools should be established for the accurate diagnosis of this disease in the different Egyptian governorates through well-structured serological surveys.

show abstract

“…Another mechanism for initiating the proteolytic cascade is induced by engagement of TNFR, Fas/APO-1/CD95, triggering caspase-3 activation by activated-caspase-8 without involvement of mitochondria [41,42]. Regarding the ligand of TNFR, TNF-α, it has been reported to be released from epithelial cells [43] and activated eosinophils [44]. Moreover, it is known that poly (ADP-ribose) polymerase (PARP) is cleaved by caspase-3, a downstream caspase of caspase-8, -9, and -12, and causes cell apoptosis [45].…”

Section: Introductionmentioning

confidence: 99%

TNF-α Mediates Eosinophil Cationic Protein-induced Apoptosis in BEAS-2B Cells

Chang

Fan

et al. 2010

BMC Cell Biol

View full text Add to dashboard Cite

BackgroundEosinophilic granulocytes are important for the human immune system. Many cationic proteins with cytotoxic activities, such as eosinophil cationic protein (ECP) and eosinophil-derived neurotoxin (EDN), are released from activated eosinophils. ECP, with low RNase activity, is widely used as a biomarker for asthma. ECP inhibits cell viability and induces apoptosis to cells. However, the specific pathway underlying the mechanisms of ECP-induced cytotoxicity remains unclear. This study investigated ECP-induced apoptosis in bronchial epithelial BEAS-2B cells and elucidated the specific pathway during apoptosis.ResultsTo address the mechanisms involved in ECP-induced apoptosis in human BEAS-2B cells, investigation was carried out using chromatin condensation, cleavage of poly (ADP-ribose) polymerase (PARP), sub-G1 distribution in cell cycle, annexin V labeling, and general or specific caspase inhibitors. Caspase-8-dependent apoptosis was demonstrated by cleavage of caspase-8 after recombinant ECP treatment, accompanied with elevated level of tumor necrosis factor alpha (TNF-α). Moreover, ECP-induced apoptosis was effectively inhibited in the presence of neutralizing anti-TNF-α antibody.ConclusionIn conclusion, our results have demonstrated that ECP increased TNF-α production in BEAS-2B cells and triggered apoptosis by caspase-8 activation through mitochondria-independent pathway.

show abstract

Cytokines in the modulation of eosinophilia

Abstract: In this review we discuss our recently results showing interleukin 5

Cited by 11 publications

References 23 publications

IFN-gamma, IL-5, IL-6 and IgE in patients infected with Giardia intestinalis.

IFN-gamma, IL-5, IL-6 and IgE in patients infected with Giardia intestinalis.

Detection of Humoral and Cellular Immune Responses in Buffaloes Naturally Infected with Sarcocystosis with Risk Factor Assessment

TNF-α Mediates Eosinophil Cationic Protein-induced Apoptosis in BEAS-2B Cells

Contact Info

Product

Resources

About